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EC number: 203-166-4 | CAS number: 104-01-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Guinea pig maximisation test was used to test the sensitizing capability of 4 methoxyphenyl acetic acid ( 104-01-8) in Hartley male guinea pigs. The shaved skin of twenty Hartley male guinea pigs was treated with the test matarial. (a solid) by intrademal injection (as a 5% solution in propylene glycol as vehicle with and without Freund's adjuvant) and then by topical application (5% in propylene glycol) eight days later. Two weeks after the final induction administration, animals were challenged with the test material as a 75% preparation in petrolatum using a 24-hour dermal exposure. Ten non-induced animak were also challenged and served as control. The site of exposure was examined for erythema and edema 24 and 48 hours following removal of the test material. 'The dose levels for challenge (75% in petrolatum) was based on a topical range-finding study to select the highest non-irritating dose which was also the highest practical dose for this material. At the 24hour examination, 1/20 animals demonstrated a slight response to the challenge dose no animal demonstrated a response at the 48 hour examination. Negative controls showed minimal response with 1/10 animals showing minimal irritation at 24 hours only. Positive control (DNCB) demonstrated a 100% response. 4-Methoxyphenylacetic Acid (MPAA) was found to be a non sensitizing to guinea pig under test condition. Therefore 4-Methoxyphenylacetic Acid (MPAA, CAS no-104 -1 -8) was considered to be a non sensitizing.
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from NTIS study report.
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Guinea pig maximisation test was used to test the sensitizing capability of 4 methoxyphenyl acetic acid in Hartley male guinea pigs.
- GLP compliance:
- not specified
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- Sufficient for classification .
- Specific details on test material used for the study:
- - Name of test material (as cited in study report):4-METHOXYPHENYL.ACETIC ACID
- Molecular formula: C9H10O3
- Molecular weight : 166.175 g/mol
- Substance type: Organic
- Physical state: Solid - Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- male
- Details on test animals and environmental conditions:
- No data available
- Route:
- intradermal and epicutaneous
- Vehicle:
- other: intradermal injection - propylene glycol as a vehicle wilh and without Freund's adjuvant Epicutaneous route- propylene glycol
- Concentration / amount:
- intradcmal injection - 5%
Epicutaneous route- 5% - Day(s)/duration:
- Epicutaneous application was performed eight days later of Intradermal induction .
- Route:
- other: Epicutaneous
- Vehicle:
- petrolatum
- Concentration / amount:
- 75%
- Day(s)/duration:
- 24 hour
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 20
- Details on study design:
- RANGE FINDING TESTS: The dose levels for challenge (75% in petrolatum) was based on a topical range -finding study to select the highest non-irritating dose which was also the highest practical dose for this material.
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 2
- Exposure period: 8 days
- Test groups:1 test group
- Control group:Yes
- Site: Not specified
- Frequency of applications: 2
- Duration:8 days
- Concentrations:
intradcmal injection - 5%
Epicutaneous route- 5%
B. CHALLENGE EXPOSURE
- Day(s) of challenge: 2 week
- Concentrations: 75% preparation in petrolatum
- Exposure period:24 hr
- Evaluation (hr after challenge): 24 and 48 hrs - Challenge controls:
- Yes
- Positive control substance(s):
- yes
- Remarks:
- DNCB (Dinitrochlorobenzene)
- Positive control results:
- 100% response was observed .
- Reading:
- other: challenge
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 5%
- No. with + reactions:
- 1
- Total no. in group:
- 10
- Clinical observations:
- Erythema and edema were observed
- Remarks on result:
- other: No sensitization was observed.
- Reading:
- other: challenge
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- 5%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- Erythema and edema were examined
- Remarks on result:
- other: Sensitization was observed.
- Reading:
- other:
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 75%
- No. with + reactions:
- 1
- Total no. in group:
- 20
- Clinical observations:
- Slight sensitization was observed
- Remarks on result:
- other: Slight sensitization was observed in one animal
- Reading:
- other: challenge
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 75%
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- No sensitization was observed.
- Remarks on result:
- no indication of skin sensitisation
- Interpretation of results:
- other: Not sensitising
- Conclusions:
- At the 24hour examination, 1/20 animals demonstrated a slight response to the challenge dose no animal demonstrated a response at the 48 hour examination. Negative controls showed minimal response with 1/10 animals showing minimal irritation at 24 hours only. Positive control (DNCB) demonstrated a 100% response.4-Methoxyphenylacetic Acid (MPAA) was considered to be a non sensitizing to guinea pig under test condition.
- Executive summary:
Guinea pig maximisation test was used to test the sensitizing capability of 4 methoxyphenyl acetic acid in Hartley male guinea pigs. The shaved skin of twenty Hartley male guinea pigs was treated with the test matarial. (a solid) by intrademal injection (as a 5% solution in propylene glycol as vehicle with and without Freund's adjuvant) and then by topical application (5% in propylene glycol) eight days later. Two weeks after the final induction administration, animals were challanged with the test material as a 75% preparation in petrolatum using a 24-hour dermal exposure. Ten non-induced animak were also challanged and Served as control. The site of exposure was examined for erythema and edema 24 and 48 hours following removal of the test material. 'The dose levels for challenge (75% in petrolatum) was based on a topical range-finding study to select the highest non-irritating dose which was also the highest practical dose for this material. At the 24hour examination, 1/20 animals demonstrated a slight response to the challenge dose no animal demonstrated a response at the 48 hour examination. Negative controls showed minimal response with 1/10 animals showing minimal irritation at 24 hours only. Positive control (DNCB) demonstrated a 100% response. 4-Methoxyphenylacetic Acid (MPAA) was found to be a non sensitizing to guinea pig under test condition. Therefore 4-Methoxyphenylacetic Acid (MPAA, CAS no-104 -1 -8) was considered to be a non sensitizing .
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
Skin sensitisation
Various studies for target (4-Methoxyphenylacetic acid, CAS No-104-01-8) and its read across were summarized as follows:
Guinea pig maximisation test was used to test the sensitizing capability of 4 methoxyphenyl acetic acid ( 104-01-8) in Hartley male guinea pigs (NTIS study report, 1991).The shaved skin of twenty Hartley male guinea pigs was treated with the test matarial. (a solid) by intrademal injection (as a 5% solution in propylene glycol as vehicle with and without Freund's adjuvant) and then by topical application (5% in propylene glycol) eight days later. Two weeks after the final induction administration, animals were challenged with the test material as a 75% preparation in petrolatum using a 24-hour dermal exposure. Ten non-induced animak were also challenged and served as control. The site of exposure was examined for erythema and edema 24 and 48 hours following removal of the test material. 'The dose levels for challenge (75% in petrolatum) was based on a topical range-finding study to select the highest non-irritating dose which was also the highest practical dose for this material. At the 24hour examination, 1/20 animals demonstrated a slight response to the challenge dose no animal demonstrated a response at the 48 hour examination. Negative controls showed minimal response with 1/10 animals showing minimal irritation at 24 hours only. Positive control (DNCB) demonstrated a 100% response. 4-Methoxyphenylacetic Acid (MPAA) was found to be a non sensitizing to guinea pig under test condition. Therefore 4-Methoxyphenylacetic Acid (MPAA, CAS no-104 -1 -8) was considered to be a non sensitizing.
The skin sensitizing potential for 4-Methoxyphenylacetic acid is estimated using OECD QSAR toolbox version 3.4. The test substance 4-Methoxy phenylacetic acid (MPAA, CAS no-104 -1 -8) is estimated to be not sensitizing in mouse.
Based on the QSAR prediction done using the Danish (Q) SAR Database, the skin sensitization was estimated to be not sensitizing on guinea pig and human for 4-Methoxyphenylacetic acid. Thus it can be concluded that the substance 4-Methoxyphenylacetic acid (104-1-8) has non skin sensitization effects and based on the CLP criteria for classification it can be classified as non sensitizer to skin sensitization effects.
In other study by (Sustainability Support Service (Europe) AB with acess rights from Givaudan UK Ltd., 1978) with similar substance (CAS No: 105-13-5) was assayed in guinea pig. A skin sensitization reaction was observed for 4-methoxybenzyl alcohol (105 -13 -5) in guinea pig at the concentration 60, 30, 10 and 3%. On day 0, 0.1ml of the test material, undiluted and, if possible or necessary of its progressively diluted solutions or emulsions or suspensions is applied to an area measuring 8cm2 on the clipped flank skin of 6-8 guinea pigs per concentration group, using 1-6 such groups for each test material .After 21 days of induction period ,challenge dose was applied. These tests are performed by applying with a pipette 0.025ml of each concentration to skin areas measuring 2 cm2, the reactions being read after 24,48 and /or 72 hours. As though weak sensitization was observed at the 60 and 30% concentration in half of the animals .No sensitization was observed at the concentration of 10 and 3%. Therefore 4-methoxybenzyl alcohol (105 -13 -5)was considered to be not sensiting in guinea pig at the concentration of 10 and 3%.
In another study by (Klecak G, Geleick H, Frey JR, 1977) with similar substance (100-51-6) was observed in male and female Himalayan white-spotted guinea pigs. Skin sensitization test was observed guinea pigs by Draize test. A dose of 0.05 ml of a 0.1% solution of the compound tested in isotonic saline was injected intradermally on day 0 and further doses of 0.1 ml each were injected on 9 alternate days (total dose = 0.95 rag). The treated animals and untreated controls were challenged intradermally with 0.05 ml of a 0.1 per cent solution on days 35 and 49. No sensitization was observed after challenge treatment. Therefore Benzyl alcohol (100 -51 -6) was considered to be not sensitizing in guinea pigs by Draize test.
On the basis of available information for the target as well as read across substance and applying weight of evidence approach, the test substance can be considered as not sensitising to the skin.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
According to CLP Regulation EC No. 1272/2008 and based on the above studies, it is concluded that the substance 4-methoxyphenylacetic acid (CAS No. 104-01-8) is not classified as skin sensitizer.
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