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EC number: 479-940-2 | CAS number: 613246-75-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-10-18 to 2004-11-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- April 4th 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- May 30th 1988
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- not applicable
- Vehicle:
- yes
- Details on test solutions:
- For the pre-test, a stock solution containing 200 mg test item/L in deionized water was prepared. After 10 minutes in the ultrasonic bath the emulsion didn't show any undissolved particles. The stock solution then was used to prepare solutions at the concentrations of 100,10, and 1 mg/L. The treatment of 1000 mg/L was prepared by adding the appropriate volume of the test item directly. In the main study, the test item was used directly.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used. The chosen plant treats mostly household sewage. The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, 67435 NW-Lachen-Speyerdorf.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- no
- Hardness:
- 0.943 mmol/L
- Test temperature:
- 20 ± 2 °C
- pH:
- 8.17
- Dissolved oxygen:
- not specified
- Salinity:
- not applicable
- Conductivity:
- not specified
- Nominal and measured concentrations:
- pre-test: 1, 10, 100, 1000 mg/L
main study: 1000 mg/L - Details on test conditions:
- 1. Test Vessels
Glass beakers were used as test vessels. They were cleaned before use. Narrow-neck glass bottles with flat bottoms were used as measuring flasks.
2. Preparation
The nutrient solution was freshly prepared; the inoculum was taken from its source, washed, aerated and the dry matter was determined. The stock solutions of test item and reference item were prepared.
3. Experimental conditions
Duration
Pre-Test: three hours
Replicates: one replicate/treatment
Control: one replicate before and one after measuring O2 concentrations in the test item replicates
Main-Study
Replicates: two replicates/treatment
Control: two replicates before, two between and two after measuring O2-concentrations in the reference and test item replicates
Water: tap water
Aeration: purified air, using Pasteur pipettes, flow approx. 0.75 L/min
Feeding: nutrient solution, 16 ml/vessel
Temperature: 20 ± 2 °C
Conduct of the study
16 mL nutrient solution were mixed with 284 mL water in the first control vessel, then 200 mL inoculum were added and the mixture was aerated. Five minutes later, the first treatment was prepared by mixing 16 mL nutrient solution with the appropriate amount of test item and adding water to give 300 mL. Then 200 mL inoculum were added and the mixture was aerated. The following reference, test item and control treatments were prepared likewise in five minute intervals. After three hours, the content of the first vessel was poured in a 250 mL narrow-neck bottle and the respiration rate was determined by measurement of the O2-concentration over a period of five minutes. The following vessels were measured likewise in five minute intervals. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenole
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL n.d.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL n.d.
- Details on results:
- Validity
The reference item showed an EC50 of 8.2 mg/L in the main study, which is within the recommended range of 5 - 30 mg/L. The O2 consumption rates of the controls showed a variation of 3 % in the pre-test and 7 % in the main study, which is well below the recommended limit of 15%. In the main study, the values at the begin and at the end of the evaluated O2 measurements were within the recommended range of 6.5 - 2.0 mg/L. - Results with reference substance (positive control):
- The EC50 of the reference item was determined as 8.2 mg/L (95% confidence interval: 6.4 - 10.2 mg/L), which lies within the recommended range of 5 - 30 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Sika Härter LJ was tested for toxicity to microorganisms by determination of inhibition of the respiration of activated sludge according to OECD guideline 209 revealing a 3h EC50 of > 1000 mg/L and a NOEC 1000 mg/L.
- Executive summary:
Sika Härter LJ was tested for toxicity to microorganisms by determination of inhibition of the respiration of activated sludge according to OECD guideline 209 and EU method C.11. Two valid experiments were performed. In the pre-test, the test item was tested using four concentrations (ranging from 1000 to 1 mg/L). Duration of the test was three hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 4.0 g suspended solids/L, giving a concentration of 1.6 g suspended solids/L in the test. As the pre-test was performed for range-finding only, no reference item was tested. No inhibition of the respiration of the activated sludge was detected in the treatments.
The main study was performed as a limit-test with a nominal concentration of 1000 mg/L. Conduct of the study was the same as in the pre-test. The dry matter of the activated sludge was determined as 4.1 g suspended solids/L, giving a concentration of 1.64 g suspended solids/L in the test. 3,5-Dichlorophenole was used as reference item. The EC50 of the reference item was determined as 8.2 mg/L (95% confidence interval: 6.4 - 10.2 mg/L), which lies within the recommended range of 5 - 30 mg/L. The following results for the test item Sika Härter LJ were determined:
NOEC 3h = 1000 mg/L
3h EC20 > 1000 mg/L
3h EC50 > 1000 mg/L
Reference
Description of key information
Sika Härter LJ was tested for toxicity to microorganisms by determination of inhibition of the respiration of activated sludge according to OECD guideline 209 revealing a 3h EC50 of > 1000 mg/L and a NOEC 1000 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Sika Härter LJ was tested for toxicity to microorganisms by determination of inhibition of the respiration of activated sludge according to OECD guideline 209 and EU method C.11. Two valid experiments were performed. In the pre-test, the test item was tested using four concentrations (ranging from 1000 to 1 mg/L). Duration of the test was three hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 4.0 g suspended solids/L, giving a concentration of 1.6 g suspended solids/L in the test. As the pre-test was performed for range-finding only, no reference item was tested. No inhibition of the respiration of the activated sludge was detected in the treatments.
The main study was performed as a limit-test with a nominal concentration of 1000 mg/L. Conduct of the study was the same as in the pre-test. The dry matter of the activated sludge was determined as 4.1 g suspended solids/L, giving a concentration of 1.64 g suspended solids/L in the test. 3,5-Dichlorophenole was used as reference item. The EC50 of the reference item was determined as 8.2 mg/L (95% confidence interval: 6.4 - 10.2 mg/L), which lies within the recommended range of 5 - 30 mg/L. The following results for the test item Sika Härter LJ were determined:
NOEC 3h = 1000 mg/L
3h EC20 > 1000 mg/L
3h EC50 > 1000 mg/L
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