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EC number: 691-232-7 | CAS number: 474266-68-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: secondary literature
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
- Principles of method if other than guideline:
- The test material was dispensed in corn oil and administered to one rat per dose per intragastric intubation. Dose rates administered ranged from 200 to 3400 mg/kg bw in increments of approx. 50%. Following administration of the test substance, rats were observed for clinical signs and toxicity. Observations for mortality were made daily throughout the study.
- GLP compliance:
- yes
- Test type:
- standard acute method
Test material
- Reference substance name:
- not available
- EC Number:
- 691-232-7
- Cas Number:
- 474266-68-7
- Molecular formula:
- not available
- IUPAC Name:
- not available
- Details on test material:
- composition:
approx. 20% diisobutylene nitrosate
approx. 80% diisobutylene
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD BR
- Sex:
- male
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Doses:
- 200, 300, 450, 670, 1000, 1500, 2300, 3400 mg/kg bw
- No. of animals per sex per dose:
- one male rat/dose
- Control animals:
- not specified
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- other: ALD = approximate lethal dose
- Effect level:
- 450 mg/kg bw
- Based on:
- test mat.
Any other information on results incl. tables
The lowest dose of diisobutylene nitrosate where one animal died was 450 mg/kg bw. The survival of the rats dosed at 670 mg/kg bw or 1000 mg/kg bw was attributed to animal variability and does not change the ALD (approximate lethal dose).
The rats dosed at 200 and 300 mg/kg bw exhibited no clinical signs of toxicity during the study. The rats dosed at 670 mg/kg bw exhibited a lump (approx. 1 cm diameter) in the abdominal area from test day 8 through the end ofthe study. The rat dosed at 1000 mg/kg bw exhibited lethargic behaviour, convulsions, red nasal discharge, ruffled fur, diarrhea and yellow stained perineum. Slight to severe weight loss (up to 12% of initial body weight) were observed in most rats one day after dosing. The rats dosed at 450, 1500 or 3400 mg/kg bw exhibited convulsions approximaetly 15 minutes after dosing. The rat dosed at 2300 mg/kg bw did not exhibit any clinical signs before death. All deaths occured within 2 hours after dosing.
Applicant's summary and conclusion
- Executive summary:
Diisobutylene nitrosate (approximately 20% pure) was administered as a single oral dose by gastric intubation to male rats. Deaths occured within 2 hours after dosing. Clinical signs of toxicity were observed in lethally and nonlethally dosed animals. Under the condition of this test, the ALD was 450 (approximately lethal dose) mg/kg bw.
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