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EC number: 227-290-3 | CAS number: 5766-67-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February-March 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data on batch no. is given; no data on the composition of the test compound. Limited reported study comparable to guideline/standard
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 798.1100 (Acute Dermal Toxicity)
- Principles of method if other than guideline:
- Method used is comparable to OECD 402.
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Ethylenediaminetetraacetonitrile
- EC Number:
- 227-290-3
- EC Name:
- Ethylenediaminetetraacetonitrile
- Cas Number:
- 5766-67-6
- Molecular formula:
- C10H12N6
- IUPAC Name:
- 2-({2-[bis(cyanomethyl)amino]ethyl}(cyanomethyl)amino)acetonitrile
- Details on test material:
- Name of test compound: ethylenediaminetetraacetonitrile
Batch no.: 1036-37-A
Appearance: white solid
Storage: room temperature```````````````````````````````````````
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Eastern Rabbit Breeding Lab, Taunton, MA, USA
- Age at study initiation: 10-12 weeks
- Weight at study initiation: 2-3 kg
- Fasting period before study: no
- Housing: individually using suspended stainless steel cages
- Diet (e.g. ad libitum): ad lib
- Water (e.g. ad libitum): ad lib
- Acclimation period: 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20±1
- Humidity (%): 30-70
- Air changes (per hr): 10-13
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 18 February To: 4 March 1993
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: ca. 10% of body surface. The test compound was directly applied onto the skin; next 2 single layer gauze
patches were applied that had been moistened with water.
- % coverage: no info
- Type of wrap if used: impervious bandaging
REMOVAL OF TEST SUBSTANCE
- Washing (if done): skin was rinsed with USP water for injection
- Time after start of exposure: immediately after patch removal
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2g/kg
- Concentration (if solution): undiluted
- Constant volume or concentration used: undiluted
- For solids, paste formed: no, patches were moistened (see above)
VEHICLE: not used - Duration of exposure:
- 24 h
- Doses:
- 2 g/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily; BW weekly
- Necropsy of survivors performed: yes - Statistics:
- Not required.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Remarks on result:
- other: no mortality
- Mortality:
- No.
- Clinical signs:
- other: Not present.
- Gross pathology:
- No data.
- Other findings:
- None.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- As no levels were tested higher than 2000 mg/kg bw (limit test), but the outcome showed that at 2000 mg/kg bw there was no mortality, no clinical signs and normal body weight gain, there is need it is expeat 5000 mg/kg bw is not known. Therefore, the test
compound was classified in Category V, according to OECD-GHS. - Executive summary:
The test substance, Ethylenediaminetetraacetonitrile, was evaluated for its potential to produce systemic toxicity or death following a 24-h topical application under occlusive conditions at a dose of 2 g/kg in a group of 5 male and 5 female New Zealand White albino rabbits. Based on the absence of mortality, the test substance is defined as non-toxic. As no levels were tested higher than 2000 mg/kg bw (limit test), the outcome at 5000 mg/kg bw is not known. Therefore, the test compound was classified in Category V, according to OECD-GHS.
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