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EC number: 276-017-4 | CAS number: 71786-67-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Benzyl(3-hydroxyphenacyl)methylammonium chloride
- EC Number:
- 276-017-4
- EC Name:
- Benzyl(3-hydroxyphenacyl)methylammonium chloride
- Cas Number:
- 71786-67-9
- Molecular formula:
- C16H17NO2.ClH
- IUPAC Name:
- benzyl(3-hydroxyphenacyl)methylammonium chloride
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): Benzyladrianon HCl
- Physical state: solid
- Analytical purity: 99.07 %
- Stability under test conditions: stable
- Storage condition of test material: At room temperature in the dark
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- Without metabolic activation : TA 98 4 µg/plate
TA 100 650 µg/plate
With metabolic activation : TA 98 + TA 100 1 µg
- Vehicle / solvent:
- Without metabolic activation : TA 98 Saline
TA 100 DMSO
With metabolic activation : TA 98 + TA 100 DMSO
Negativ control : dimethylsulphoxide
Positiv control : - Saline
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Saline and DMSO
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar
DURATION
- Preincubation period: Until the cultures reached an O.D. of 0.4 at 700 nm
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 2 - Evaluation criteria:
- A substance is considered positive ( mutagenic ) in the test if it induces at least a 2-fold, dose related increase in the number of revertants with
respect to the number induces by the solvent control in any other of the tester strains, either with or without metabolic actiavtion. However, any
mean plate count of less than 20 is considered to be not significant.
The preceding criteria were not absolute and other modifying factors might enter into the final evaluation decision.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: no - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
Based on the results of this study it is concluded that Benzyladrianon HCl is mutagenic in the Salmonella typhimurium reverse mutation assay. - Executive summary:
Benzyladrianon HCl was tested in the Salmonella typhimurium reverse mutation assay with two histidine-requiring strains of Salmonella typhimurium ( TA 100 and TA 98 ). To obtain more information about the mutagenicity, an additional test was performed with strain TA 98 in the absence of S9-mix and with strain TA 100 in absence and presence of S9-mix.
In the first experiment, Benzyladrianon HCl was tested up to concentrations of 3000 µg/plate in the absence of S9 -mix in strain TA98. Benzyladrianon HCl was tested up to concentrations of 4000 and 5000 µg/plate in the absence and presence of S9 -mix respectively in strain TA 100. Toxicity was observed at these dose levels.
Benzyladrianon HCl did not precipitate on the plate at this dose level.
In tester strain TA98 in the presence of S9 -mix, Benzyladrianon HCl showed negative responses over the entire dose range, i.e. no dose related, two-fold increase in the number of revertants.In the absence of S9 -mix, Benzyladrianol HCl induced up to 1.9 -and 2.0 -fold, not dose related, increases in the number of revertant colonies compared to the solvents control in the first and second experiment respectively.
In tester strain TA100 in the absence of S9 -mix, Benzyladrianon HCl induced up to 2.1 -and 4.5 -fold, dose related, increases in the number of revertant colonies compared to the solvents control in the first and second experiment respectively.
In the presence of S9 -mix, Benzyladrianon HCl induced up to 3.2 -and 3.0 -fold, dose related, increases in the number of revertant colonies compared to the solvents control in the first and second experiment respectively.
Based on the results of this study it is concluded that Benzyladrianon HCl is mutagenic in the Salmonella typhimurium reverse mutation assay.
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