Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 245-485-1 | CAS number: 23202-81-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation / corrosion
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 December 2012 to 09 July 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Fully GLP compliant and in accordance with current test guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.40 (In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test (TER))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.46 (In Vitro Skin Irritation: Reconstructed Human Epidermis Model Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methylfuranoside
- IUPAC Name:
- Methylfuranoside
- Reference substance name:
- Methyl 5-deoxy-2,3-O-isopropylidene-β-D-ribofuranoside
- EC Number:
- 245-485-1
- EC Name:
- Methyl 5-deoxy-2,3-O-isopropylidene-β-D-ribofuranoside
- Cas Number:
- 23202-81-5
- Molecular formula:
- C9H16O4
- IUPAC Name:
- methyl 5-deoxy-2,3-O-isopropylidene-beta-D-ribofuranoside
- Test material form:
- other: plae yellow liquid
- Details on test material:
- Name: Methylfuranoside
CAS number: 23202-81-5
Batch number: 201111082008
Quantitiy received: 1.5 kg
Purity: 99.7%
Expiry date: November 2013
Date of receipt: 19 November 2013
Appearance: pale yellow liquid
Storage details: stored in a sealed container, at room temperature (15 to 25°C) in the dark.
The negative control substance for the corrosivity test was purified water supplied by Baxter Healthcare Ltd, Thetford, UK.
The positive control substance for the corrosivity test was 8N potassium hydroxide supplied by Sigma-Aldrich Co. Ltd., Poole, UK.
The negative control substance for the irritation test was phosphate buffered saline (PBS) (pH 7.4) supplied by MatTek Corporation, Ashland, Massachusetts, USA.
The positive control substance for the irritation test was 5% w/v sodium dodecyl sulphate (SDS) supplied by Severn Biotech Limited, Kidderminster, UK.
Constituent 1
Constituent 2
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- other: Three-dimensional human skin model, comprising a reconstructed epidermis with a functional stratum corneum supplied by MatTek Corporation, Ashland, Massachusetts, USA.
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- Three-dimensional human skin model, comprising a reconstructed epidermis with a functional stratum corneum supplied by MatTek Corporation, Ashland, Massachusetts, USA. Human keratinocytes are used to construct the epithelium. Multiple layers of viable epithelial cells are present under a functional stratum corneum. The stratum corneum is multi-layered with the necessary lipid profile to produce a functional barrier. The containment properties of the model prevent the passage of material around the stratum corneum to the viable model tissue. The skin model was supplied free of contamination with bacteria, mycoplasma and fungi.
SKIN CORROSION
EpiDermTM inserts were treated with the test item, negative control and positive control over two time points: three minutes and one hour. At the end of the treatment period, the tissues were washed with PBS and cell viability was assessed using the MTT assay. The skin corrosion potential was classified according to the remaining cell viability obtained after test item treatment.
The test item was considered not to be corrosive. The irritation test was therefore conducted.
Skin irritation: After dosing the tissues were incubated at 37 ± 1ºC, 5 ± 1% CO2 for 25 ± 1 minute. Although the tissues were removed from the incubator 10 minutes earlier than required by the protocol, they were left for a total of 1 hour from initiation of treatment to start of wash-off as stated in the protocol. This deviation from protocol was considered not to have affected the integrity or outcome of the study as the data confirms that the controls met the acceptance criteria. The plates were removed from the incubator and placed into a sterile hood until the 60 ± 1 minute treatment period was complete for each tissue. The tissues were washed using PBS and dried using cotton wool buds to remove residual material before being transferred to a new well containing prewarmed
maintenance medium. The tissues were then incubated at 37°C for the 42 ± 2 hour recovery time period.
Upon completion of the 42 hour recovery period, the base of each tissue was rinsed with PBS before being placed on top of 0.3 mL of of MTT solution (1 mg/mL) and incubated for three hours (37°C, 5% CO2).
Skin corrosion: The plates were removed from the incubator and placed into a sterile hood until the 60 ± 1 minute treatment period was complete for each tissue. The tissues were washed using PBS and dried using cotton wool buds to remove residual material before being transferred to a new well
containing pre-warmed maintenance medium. The tissues were then incubated at 37°C for the 42 ± 2 hour recovery time period.
Upon completion of the 42 hour recovery period, the base of each tissue was rinsed with PBS before being placed on top of 0.3 mL of of MTT solution (1 mg/mL) and incubated for three hours (37°C, 5% CO2).
Upon completion of the 42 hour recovery period, the base of each tissue was rinsed with PBS before being placed on top of 0.3 mL of of MTT solution (1 mg/mL) and incubated for three hours (37°C, 5% CO2). After incubation, the tissues were washed with PBS Any resultant colour was extracted
by flooding the tissue with 2 mL isopropanol and shaking at 150 rpm for two hours. Upon completion of the extraction, each tissue was pierced using a hypodermic needle so that the extract could run through the tissue. Once drained, the tissue was discarded and the extract mixed by pipette. Two x 200 µL aliquots of each resultant extract were placed into a 96-well plate for spectrophotometric determination of optical density at 570 nm using extraction solution as a blank. Tissue viability was calculated for each tissue as a percentage of the mean of the negative control tissues
ASSESMENT STAINING POTENTIAL FOR NON-COLOURED SUBSTANCES
30 µL of the test item was added to 0.3 mL deionised water in a suitable glass container and incubated for 60 minutes at 37 ± 1ºC, 5 ± 1% CO2. At the end of the incubation, the mixture was shaken. There was no staining, therefore the test item was deemed not to have the potential to stain the tissue.
ASSESSMENT OF MTT INTERACTING SUBSTANCES
In order to assess the potential non-specific reduction of the test item, 30 µL of test item was added to 1.0 mL of 1.0 mg/mL MTT and the colour change was assessed after incubation for 60 minutes at 37 ± 1ºC, 5 ± 1% CO2. There was no change in colour therefore the test item did not interact with MTT. - Amount/concentration applied:
- 30 µL of the undiluted test item
- Duration of treatment / exposure:
- Skin corrosion: two exposure times of 3 minutes and 1 hour.
Skin irritation: 25 ± 1 minute. - Duration of post-treatment incubation (if applicable):
- 42 hour
- Number of replicates:
- Triplicates
Test system
- Type of coverage:
- other: Not applicable
- Preparation of test site:
- other: Not applicalbe
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 1- 3 min exposure
- Value:
- ca. 89
- Positive controls validity:
- valid
- Remarks:
- 19% viability
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 1- 60 min exposure
- Value:
- ca. 60
- Positive controls validity:
- valid
- Remarks:
- 9% viability
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- irritation (OECD 439) 2 - 3 min exposure
- Value:
- 90.5
- Negative controls validity:
- valid
- Remarks:
- 100 % viability
- Positive controls validity:
- valid
- Remarks:
- 6.7 % viability
- Other effects / acceptance of results:
- Skin corrosion
Skin viability after a three minute or one hour exposure to the test item was 89% and 60%, respectively.
Skin viability after a three minute or one hour exposure to the positive control item was 19% and 9%, respectively, demonstrating appropriate performance of the assay.
Skin irritation
The group mean viability for the test item was 90.5%, for the negative control was 100% and for the positive control was 6.7%.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test item, Methylfuranoside, was considered not to be corrosive or irritating to the in vitro skin models.
- Executive summary:
The purpose of the study was to determine whether the test item Methylfuranoside caused dermal corrosion or irritation. Dermal corrosion refers to the production of irreversible tissue damage in the skin following the application of a test material. Dermal irritation refers to the production of reversible inflammatory changes in the skin following the application of a test material.
The potential for chemical induced skin corrosion or irritation is an important consideration in establishing procedures for safe handling, packing and transport of chemicals.
The corrosivity potential of the test item was evaluated using the EpiDerm test system. The irritation test was conducted using the EpiDerm SIT (EPI-200) test system.
In the corrosivity test, skin viability after a three minute or one hour exposure to the test item was 89% and 60%, respectively.
In the irritation test, the group mean viability for the test item was 90.5%, for the negative control was 100% and for the positive control was 6.7%.
The test item, Methylfuranoside, was considered not to be corrosive or irritating to skin.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.