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EC number: 478-310-4 | CAS number: 53803-13-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 2017 - December 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Lot number: 13AP016
Expiration date: 2018.5.1
Purity: >99%
Appearance: White Solid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Activated sludge microorganisms were obtained from a Chengdong waste water treatment plant receiving predominantly domestic sewage.
On arrival at the laboratory, a small amount of the fresh sludge was weighed, oven-dried, and reweighed.
A calculation was made from these results to determine the amount of wet sludge that must be suspended in deionized water in order to obtain an activated sludge with a mixed-liquor suspended solids (MLSS) level of approximately 3 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- NA
- Test temperature:
- 20±2 degrees celcius
- pH:
- 7.02 - 7.85
- Dissolved oxygen:
- The mixture was sealed and shaken to keep aerobic condition.
After 3h, a standard BOD measuring bottle was filled from the contents of the first beaker and the respiration rate was measured over a period of 10 min, or until the dissolved oxygen level reached 2.00 mg/L. - Salinity:
- NA
- Conductivity:
- NA
- Details on test conditions:
- Sludge microorganisms were exposed to three concentrations of the test substance at nominal concentration of 10, 100 and 1000 mg/L in range-finding test. The respiration rate, expressed as oxygen consumption by the microbes in mg 0 2 per liter per hour, was measured under defined conditions following the 30min exposure period. Inhibition values were calculated by comparing test respiration rates to negative control rates.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- The result indicated that under valid test conditions, there was no obvious oxygen uptake of abiotic blank of test substance for dissolved oxygen was 8.47 and 8.47 mg 0 2/L at the beginning and end of test.
- Results with reference substance (positive control):
- The 3h-EC50 value of 3,5-dichlorophenol was 14.0 mg/L, and 95% confidence limit ranging from 11.7mg/L to 16.7mg/L, which was in the range 2 mg/L to 25 mg/L.
The oxygen uptake rates of negative controls were 94.1 and 87.3 mg 0 2/(L·h), which were 62.7 and 58.2 mg 0 2/(g·h) after considering the concentration of sludge of 1.5 g/L, fulfilled the validity criterion of greater than 20 mg 0 2/(g·h). Additionally, the coefficient of variation of the two negative control respiration rates was 4.50%, which was within 30% of each other. The validity criteria were fulfilled - Validity criteria fulfilled:
- yes
- Conclusions:
- In an OECD 209 "Activated Sludge, Respiration Inhibition Test" , the 3h-EC50 value of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1: 1)) was greater than 1000 mg/L.
The result of range-finding test showed that the 3h-EC50 of the test substance was great than 1000 mg/L, there was no need to launch the definitive test. - Executive summary:
In an OECD209"Activated Sludge, Respiration Inhibition Test" , sludge microorganisms were exposed to three concentrations of the test substance at nominal concentration of 10, 100 and 1000 mg/L in range-finding test.
The respiration rate, expressed as oxygen consumption by the microbes in mg 0 2 per liter per hour, was measured under defined conditions following the 30min exposure period. Inhibition values were calculated by comparing test respiration rates to negative control rates.
The result indicated that under valid test conditions, there was no obvious oxygen uptake of abiotic blank of test substance for dissolved oxygen was 8.47 and 8.47 mg 0 2/L at the beginning and end of test. The 3h-EC50 value of 3,5-dichlorophenol was 14.0 mg/L, and 95% confidence limit ranging from 11.7mg/L to 16.7mg/L, which was in the range 2 mg/L to 25 mg/L. The oxygen uptake rates of negative controls were 94.1 and 87.3 mg 0 2/(L·h), which were 62.7 and 58.2 mg 0 2/(g·h) after considering the concentration of sludge of 1.5 g/L, fulfilled the validity criterion of greater than 20 mg 0 2/(g·h). Additionally, the coefficient of variation of the two negative control respiration rates was 4.50%, which was within 30% of each other. The validity criteria were fulfilled.
Under the above valid test conditions, the 3h-EC50 value of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1: 1)) was greater than 1000 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because the substance is found to be readily biodegradable and the applied test concentrations are in the range of concentrations that can be expected in the influent of a sewage treatment plant
Referenceopen allclose all
Description of key information
In an OECD 209 "Activated Sludge, Respiration Inhibition Test" , the 3h-EC50 value of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1: 1)) was greater than 1000 mg/L.
The result of range-finding test showed that the 3h-EC50 of the test substance was great than 1000 mg/L, there was no need to launch the definitive test.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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