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EC number: 232-576-6 | CAS number: 9001-03-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 September 2022 to January 11 2023
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Remarks:
- Experiment 2 only one replicate for the treatment groups.
- Qualifier:
- according to guideline
- Guideline:
- ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
- Deviations:
- no
- Remarks:
- Experiment 2 only one replicate for the treatment groups.
- Principles of method if other than guideline:
- Only nominal concentrations.
- GLP compliance:
- yes
- Remarks:
- non-GLP study
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations:
Experiment 1: 42.3, 84.6, 169.2, 338.3, 676.6 mg enzyme concentrate dry matter/L.
Experiment 2: 8.4, 25.1, 75.2, 225.5, 676.6 mg enzyme concentrate dry matter/L. - Vehicle:
- no
- Details on test solutions:
- According to the MicroBiotests Inc SOP.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Unicellular green algae
- Strain: Pseudokirchneriella subcapitata
- Source (laboratory, culture collection): MicroBiotests Inc. The algal growth inhibition bioassay has been developed by the research teams at the Laboratory for Biological Research in Aquatic Pollution (LABRAP) at the University of Ghent in Belgium.
- Method of cultivation: According to the MicroBiotests Inc. SOP.
Algae are supplied as de-immobilized algal beads. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- As described in OECD Guideline 201and the ISO/DIS Guideline 8692.
- Test temperature:
- As described in OECD Guideline 201and the ISO/DIS Guideline 8692.
- pH:
- 7.2-7.6
- Nominal and measured concentrations:
- Nominal concentrations
- Details on test conditions:
- TEST SYSTEM
Experiment 1:
- Test vessel: 25 mL calibrated flask
- Material, size, headspace, fill volume: 25 mL
- Initial cells density: 1*10^4 algae/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
Experiment 2:
Due to precipitations seen in experiment 1, the batch was sterile filtered in experiment 2. 3x 100 mL vials for the controls, 1X 100 mL vials for each test concentration. Vials were loosely covered with parafilm and were under constant shaking.
GROWTH MEDIUM
- Standard medium used: yes. An amount of 250 mg sodium bicarbonate will be added to the algae medium, before preparing the test concentrations, to account for the CO2 quenching mechanism of Carbonic anhydrase.
- Detailed composition if non-standard medium was used: As described in OECD Guideline 201and the ISO/DIS Guideline 8692.
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 72 h
- Light intensity and quality: 10000 lux for sideway illumination or min. 3000-4000 lux for bottom illumination
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 59.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: enzyme concentrate dry matter
- Basis for effect:
- growth rate
- Details on results:
- In experiment 1, there were a lot of precipitates from the batch present, and it was difficult to distinguish under the microscope between algae cells and precipitates. The batch was sterile filtered in experiment 2, and the beakers were under constant shaking and containing 250 mg sodium bicarbonate. This helped with algae cells counting. Triplicates for the control group and single replicates for the treatment groups.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The exposure of R. subcapitata to Carbonic anhydrase, batch PPE93985 was toxic to algae based on its algaestatic, CO2 quenching mode of action. 250 mg sodium bicarbonate and constant shaking were introduced to the test system. 72-hr EC50 was 59.4 mg enzyme concentrate dry matter, equivalent to 8.8 mg active enzyme protein/L. The study is non-GLP and analytical measurements have not been undertaken.
- Executive summary:
The purpose of this study was to screen for toxicity of Carbonic anhydrase, batch PPE93985 towards algae. A 72h algal growth inhibition test was performed in long cell test vials with R. subcapitata, followed by a second experiment in 100 mL vials by constant shaking. Algae are supplied as de-immobilized algal beads. The Algal toxkit test follows the prescriptions of the OECD "Algal growth inhibition test" (Guideline 201) and the ISO "Water Quality - Freshwater Algal Growth Inhibition Tests with Unicellular Green Algae" (ISO Standard 8692). Endpoint of the study is percent inhibition of algal growth rate compared to the control.
Due to precipitations seen in experiment 1, the batch was sterile filtered in experiment 2. 3x 100 mL vials for the controls, 1X 100 mL vials for each test concentration. Vials were loosely covered with parafilm and were under constant shaking. Concentrations used were:
Experiment 1: 42.3, 84.6, 169.2, 338.3, 676.6 mg enzyme concentrate dry matter/L.
Experiment 2: 8.4, 25.1, 75.2, 225.5, 676.6 mg enzyme concentrate dry matter/L.The exposure of R. subcapitata to Carbonic anhydrase, batch PPE93985 was toxic to algae based on its algaestatic, CO2 quenching mode of action. 250 mg sodium bicarbonate and constant shaking were introduced to the test system in experiment 2. 72-hr EC50 was 59.4 mg enzyme concentrate dry matter, equivalent to 8.8 mg active enzyme protein/L. The study is non-GLP and analytical measurements have not been undertaken.
Reference
Description of key information
The exposure of R. subcapitata to Carbonic anhydrase, batch PPE93985 was toxic to algae based on its algaestatic, CO2 quenching mode of action. 250 mg sodium bicarbonate and constant shaking were introduced to the test system. 72-hr EC50 was 59.4 mg enzyme concentrate dry matter, equivalent to 8.8 mg active enzyme protein/L. The study is non-GLP and analytical measurements have not been undertaken.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 59.4 mg/L
Additional information
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