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EC number: 419-560-6 | CAS number: 4369-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 March to 24 March 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- Previous version (1992)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 797.1400 (Fish Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and all test concentrations
- Sampling method: Water samples were taken and extracted immediately upon preparation. No further details on sampling provided
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test material was prepared by direct dispersion in water. 20 g was dispersed in dechlorinated tap water with the aid of ultrasonic disruption and shaking and the volume adjusted to 5 litres to give a 4.0 g/l aqueous stock solution. Aliquots of this stock solution were added to dechlorinated tap water to give the test series.
- Eluate: not reported
- Differential loading: no
- Controls: dechlorinated tap water
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None reported
- Other relevant information: None - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Strain: not reported
- Source: Donnington Fish Farm, Upper Swell, Gloucester, U.K.
- Age at study initiation (mean and range, SD): not reported
- Length at study initiation (length definition, mean, range and SD): mean = 4.4 cm (SD 0.1 cm)
- Weight at study initiation (mean and range, SD): 0.46 g (SD 0.05 g)
- Method of breeding: not reported
- Maintenance of the brood fish: not reported
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation: fish were fed commercial trout pellets
- Feeding frequency during acclimation: not reported, however, feeding was stopped 48 hours prior to the start of the test
- Health during acclimation (any mortality observed): <1% mortalities
FEEDING DURING TEST - fish were not fed during test - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- approximately 112 mg/l as CaCO3
- Test temperature:
- 14 +/- 1 degrees C
- pH:
- 7.3-7.4
- Dissolved oxygen:
- 9.2 - 10.1
- Salinity:
- Not reported
- Conductivity:
- Not reported
- Nominal and measured concentrations:
- Nominal: control, 10, 18, 32, 56 and 100 mg/l
Measured: see results section (high variation between samples measured at 0 h, 24 h and 96 h). - Details on test conditions:
- TEST SYSTEM
- Test vessel: 20 L glass vessels
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, 20 L
- Aeration: not reported
- Renewal rate of test solution (frequency/flow rate): test solution was renewed every 24 hours, no further details reported
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.23 g bodyweight /L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Total organic carbon: Typical TOC from tests conducted on tap water from 1/1/1994 - 31/12/1994: 1.8 mg/l
- Particulate matter: not reported
- Metals: Many metals analysed from tests conducted on tap water from 1/1/1994 - 31/12/1994, none were found in excessive concentrations.
- Pesticides: Typical total pesticides from tests conducted on tap water from 1/1/1994 - 31/12/1994: <0.03 µg/l
- Chlorine: Typical water total chlorine from tests conducted on tap water from 1/1/1994 - 31/12/1994: 0.23 mg/l
- Alkalinity: Typical water alkalinity from tests conducted on tap water from 1/1/1994 - 31/12/1994: 104 mg/l
- Ca/mg ratio: not reported
- Conductivity: Typical water conductivity from tests conducted on tap water from 1/1/1994 - 31/12/1994: 374 M/CSM
- Hardness: Typical water hardness from tests conducted on tap water from 1/1/1994 - 31/12/1994: 175 mg CaCO3 /l
- Salinity: not reported
- Culture medium different from test medium: no
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH: adjusted to 7.5
- Photoperiod: 16 hours light : 8 hours dark
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and behaviour
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: One range finding study was conducted with the test concentrations of 1.0, 10 and 100 mg/l as a static test. Another range-finding study was conducted at 10 and 100 mg/l using flow through conditions. A final range-finding study used the test concentrations of 32 and 100mg/l and used an auxiliary solvent.
- Results used to determine the conditions for the definitive study: The first study showed mortalities at 100 mg/l. The second showed mortalities at 100 mg/l and none at 10 mg/l. The flow-through method was inappropriate due to a globule of test material forming, therefore a semi-static method was selected for the definitive study. The third resulted in precipitate, therefore a solvent was not used in the definitive test. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.I. 18-32 mg/l (based on the concentrations resulting in 0 and 100% mortality respectively)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.I. 1.5-2.4 mg/l
- Remarks:
- based on measured test solutions at 24h
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: based on measured test solutions at 24h
- Details on results:
- - Behavioural abnormalities: behaviour was observed and responses occurred at concentrations of > 18 mg/l. These included loss of equilibrium, swimming at the surface and swimming at the bottom of the test vessels.
- Observations on body length and weight: not measured
- Other biological observations: see Results
- Mortality of control: 0% after 96 hours
- Other adverse effects control: none reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none reported
- Effect concentrations exceeding solubility of substance in test medium: none reported - Reported statistics and error estimates:
- LC50 values and confidence limits were calculated by the moving average method of Thompson 1947
- Sublethal observations / clinical signs:
Table 1. Cumulative mortality in the first range-finding study (static conditions)
Nominal concentration (mg/l)
Cumulative mortality
24h
48h
72h
96h
Control
0
0
0
0
1.0
0
0
0
0
10
0
0
0
0
100
1*
3
3
3
*loss of equilibrium and swimming at bottom
Table 2. Cumulative mortality in the second range-finding study (flow-through conditions)
Nominal concentration (mg/l)
Cumulative mortality
24h
48h
72h
96h
Control
0
0
0
0
10
0
0
0
0
100
0*
3
3
3
* Hyperactive swimming
Table 3. Cumulative mortality in the definitive exposure
Nominal concentration (mg/l)
Replicate
Cumulative mortality
3h
6h
24h
48h
72h
96h
Control
1
0
0
0
0
0
0
2
0
0
0
0
0
0
10
1
0
0
0
0
0
0
2
0
0
0
0
0
0
18
1
0
0
0
0
0
0
2
0
0
0
0
0
0
32
1
0
0
0
0
1
10
2
0
0
0
0
1
10
56
1
0
0
0
10
10
10
2
0
0
0
10
10
10
100
1
0
0
10
10
10
10
2
0
0
10
10
10
10
Table 4. Behavioural observations in the definitive study
Nominal concentration (mg/l)
Replicate
Behavioural responses
Time
3h
6h
24h
48h
72h
96h
Control
1
None detected
2
None detected
10
1
None detected
2
None detected
18
1
None detected
2
None detected
32
1
Swimming at surface
Loss of equilibrium
Swimming at bottom
10/10
2/9
7/9
All dead
2
Swimming at surface
Loss of equilibrium
Swimming at bottom
6/10
3/9
6/9
All dead
56
1
Swimming at surface
6/10
6/10
All dead
All dead
All dead
2
Swimming at surface
6/10
6/10
All dead
All dead
All dead
100
1
Swimming at surface
Loss of equilibrium
8/10
2/10
All dead
All dead
All dead
All dead
2
Swimming at surface
Loss of equilibrium
8/10
2/10
All dead
All dead
All dead
All dead
Table 4. Verification of test concentrations in the definitive study
Samples
Nominal concentration (mg/l)
Measured concentration (mg/l)
% of nominal concentration
0 hours (fresh media)
Control
<LOD
-
10
10.2
102
18
17.5
97
32
30.8
96
56
52.0
93
100
88.1
88
24 hours (expired media)
Control
<LOD
-
10
1.13
11
18
2.07
11
32
2.81
9
56
5.67
10
100
10.3
10
96 hours (expired media)
Control
<LOD
-
10
0.545
5
18
0.973
5
32
1.89
6
- Validity criteria fulfilled:
- yes
- Remarks:
- Analytical measurement of the test concentrations was conducted; mortality was 0% in the control group and dissolved oxygen was approximately 100% of air saturation value (as calculated by reviewer)
- Conclusions:
- A 96-hour LC50 value of 24 mg/l was reported for the effects of the 3-(trimethoxysilyl)propyl acrylate on the mortality of Oncorhynchus mykiss in a semi-static test, based on measured concentrations.
Reference
Description of key information
96-hour: LC50 24 mg/l based on the mortality of Oncorhynchus mykiss, based on nominal concentrations. The LC50 is equivalent to 19.7 mg/l when expressed in terms of concentration of the silanol hydrolysis product, 3-acryloxypropylsilanetriol.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 19.7 mg/L
Additional information
A 96-hour LC50 value of 24 mg/l based on the mortality of Oncorhynchus mykiss was determined in a reliable guideline study, conducted under semi-static conditions with the registration substance, 3-(trimethoxysilyl)propyl acrylate (CAS 4369-14-6, EC 419-560-6), in accordance with OECD Test Guideline 203. A NOEC value of 18 mg/l was also determined in the same study. Gas chromatography analysis determined concentrations of the parent test substance were within 80-120% of nominal values at 0 hours, however only 5% of the nominal concentrations was detected after 96 hours. This is considered to reflect the loss of the parent substance due to hydrolysis. The results are therefore expressed in terms of the nominal concentrations of the test substance.
The test substance is subject to rapid hydrolysis and under the test conditions it is therefore likely that exposure will have been to its hydrolysis products (3-acryloxypropylsilanetriol and methanol).
The results may be expressed in terms of concentration of the silanol hydrolysis product, 3-acryloxypropylsilanetriol, by applying a molecular weight correction: (MW of silanol = 192.25 / MW of parent = 234.33) * [Concentration of parent = 24 mg/l] = 19.7 mg/l
There is no basis to expect that methanol significantly influences the results of the test. The toxicity of methanol is discussed further in Section 6.1 Aquatic Toxicity endpoint summary of IUCLID.
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