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Diss Factsheets
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EC number: 944-490-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: COLIPA program on optimization of existing in vitro eye irritation assays for entry into formal validation: technology transfer and intra/inter laboratory evaluation of epiocular assay for chemicals
- Version / remarks:
- test realized before OECD 492 adopted in July 2015
- Principles of method if other than guideline:
- The test is based on cytotoxicity measurement. Cellular viability is quantified by MTT reduction in a tetrazolium salt (fomazan) by viable cells. Fomazan forms violet cristals which are soluble in propan-2-ol.
- GLP compliance:
- no
Test material
- Reference substance name:
- 1-hydroxyoctan-2-yl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12-dienoate; octane-1,2-diol
- EC Number:
- 944-490-2
- Molecular formula:
- unknown
- IUPAC Name:
- 1-hydroxyoctan-2-yl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12,15-trienoate; 2-hydroxyoctyl octadeca-9,12-dienoate; octane-1,2-diol
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Yellow oil
Test animals / tissue source
- Species:
- human
- Strain:
- not specified
- Details on test animals or tissues and environmental conditions:
- Reconstructed cornea epitheliums of 0.5 cm² (EpiOcular)
Test system
- Vehicle:
- other: paraffin oil
- Controls:
- yes, concurrent vehicle
- yes, concurrent positive control
- yes, concurrent negative control
- Amount / concentration applied:
- 1% and 10%
- Duration of treatment / exposure:
- 30 +/- 2 minutes
- Duration of post- treatment incubation (in vitro):
- 30 +/- 2 minutes then post-incubation for 120 +/- 10 minutes
- Number of animals or in vitro replicates:
- 2 replicates/treatment
- Details on study design:
- Reconstructed cornea epitheliums were wetted for 30 minutes with 20 μL of PBS and treated with 50 μL of the test item for 180 minutes.
They were rinsed, immersed for 12 minutes in culture medium, dried and cultured back during 2 hours.
A viability test was then realised with the MTT. The viability percentage of treated tissues was calculated compared to the negative control in order to classify the test substance.
Results and discussion
In vitro
Resultsopen allclose all
- Irritation parameter:
- other: cellular viability
- Run / experiment:
- 10%
- Value:
- ca. 100
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
- Remarks:
- Cell viability expressed as a %
- Irritation parameter:
- other: cellular viability
- Run / experiment:
- 1%
- Value:
- ca. 100
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
- Remarks:
- Cell viability expressed as a %
- Other effects / acceptance of results:
- Positive and negative controls were validated, experiment was accepted.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Non irritant at 1% and 10%.
- Executive summary:
In a Reconstructed Human Cornea-like Epithelium (RhCE) Test Method similar to OECD 492, test substance was non irritant at 1% and 10%.
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