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EC number: 237-857-7 | CAS number: 14024-56-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- Deviations from the study plan:
Regulatory Requirements:
In deviation to the Study Plan, also the Toxic Substances Control Act (TSCA, 1975) was included in the Regulatory Requirements. This deviation did not have any impact on test results.
Test item
EEC number is 628-792-9, as declared by the Sponsor. This deviation did not have any impact on test results. - Deviations:
- yes
- Remarks:
- See in version/remarks above.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Bis(pentane-2,4-dionato-O,O')magnesium
- EC Number:
- 237-857-7
- EC Name:
- Bis(pentane-2,4-dionato-O,O')magnesium
- Cas Number:
- 14024-56-7
- Molecular formula:
- C10H14MgO4
- IUPAC Name:
- magnesium;4-oxopent-2-en-2-olate
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of test media were taken at time 0 and after 72 hours; final samples were obtained mixing the replicates and they were immediately centrifuged to separate the algae from the medium.
Control samples were analysed at the same time to verify the absence of test item contamination.
Samples were labelled and analysed immediately after sampling.
Duplicate samples of the test water and the negative control were taken from the test solutions; one sample was analysed, while the respective duplicates were stored as retain samples to be analysed only if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test was performed at five test item concentrations in a geometric series (differing by a constant factor equal to 3.2), namely 1.0, 3.1, 9.8, 31.3 and 100.0 mg/L of test item
test solutions preparation:
Test solution 1:
-nominal test item solution concentration : 1mg/L
-final volume : 200mL
-added volume of test item stock solution 100.0 mg/L: 2mL
Test solution 2:
-nominal test item solution concentration : 3.1mg/L
-final volume : 200mL
-added volume of test item stock solution 100.0 mg/L: 6.2mL
Test solution 3:
-nominal test item solution concentration : 9.8mg/L
-final volume : 200mL
-added volume of test item stock solution 100.0 mg/L: 19.6
Test solution 4:
-nominal test item solution concentration : 31.3mg/L
-final volume : 200mL
-added volume of test item stock solution 100.0 mg/L: 62.6mL
Experimental conditions
The treatment vessels were identified by experiment number, group and replicate number.
All the described procedures were conducted under a laminar flow bench to ensure the sterility of the algal cultures.
For the test concentrations and for the negative control, 100 mL capacity conical glass flasks capped with air permeable stoppers were used.
Three replicates for each test concentrations and six for negative control were prepared. At test start, 50 mL of test solutions were poured into the test flasks and inoculated with algae to obtain a starting cell density of about 10^4 cells/mL.
The inoculum was taken from a pre-culture started 3 days before the test beginning (containing approximately 10^6 cells/mL), in order to ensure that the assay was performed with an exponentially growing population.
The test flasks were incubated in an environmental test chamber, under continuous illumination and constant shaking. The flasks were randomly distributed under the light and replaced every day during the test.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24.0 – 24.1 °C
- pH:
- pH of test medium: 7.88 – 8.91 at the test start
7.35 – 8.13 at the test end - Conductivity:
- < 5 μScm^-1)
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- >= 8.7 - <= 9.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- >= 11.3 - <= 12.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 22.3 - <= 27.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 2.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- ca. 8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The toxicity to aquatic algae and cyanobacteria test was performed according to the OECD Guideline 201 (Alga, Growth Inhibition Test).
The EC50 (72h) in algae Pseudokirchneriella subcapitata was between 22.3 and 27.4mg/L.
The NOEC was 2.4 mg/L and the LOEC was 8mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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