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EC number: 241-659-6 | CAS number: 17675-60-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Sept 2017 to 01 Dec 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992. OECD (2010): No.126 Short Guidance On The Threshold Approach for Acute Fish Toxicity, 2010.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 16VL8189
- Expiration date of the lot/batch: 04 Feb 2018
- Purity test date: 05 Aug 2016
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ambient (=< 30 deg C), dark, dry - Analytical monitoring:
- yes
- Details on sampling:
- Analytical samples were taken from limit test concentration and control in 24-hours intervals. Analytical samples from control and the limit test concentration 35.0 mg/L were analysed at t = 0 h and t = 96 h
- Vehicle:
- no
- Details on test solutions:
- The necessary amount of test item for preparing the stock solution was weighed on a weighing scoop and transferred to a volumetric flask. Test medium was added up to the bench mark and the solution was homogenized by shaking and by ultrasonication for 10 minutes. Afterwards, the solution was clear and transparent. The solution was applied to the respective aquarium.
Nominal conc in test vessel: 35 mg/L
Test item required: 70mg
Final volume/application voume per test vessel: 2000 mL
Test solution per test vessel: 2 L - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Fish from the own stock breeding of the test facility were used. The parent animals were originally received from the University of Heidelberg (Heidelberg, Germany) in a healthy condition. According to the supplier they had been bred from wild strain (WA) zebrafish before. Since they have been purchased, the zebrafish were continuously reared in the laboratory of the testing facility. Only fish in good health and free from any apparent malformation were used. The last feeding was performed three days prior test start. The holding water was composed of reconstituted test water consisting of analytical grade salts dissolved in purified water. The ratio of Ca:Mg and Na:K was 4:1 and 10:1, respectively, based on molarity. The water parameters were controlled 3 times a week. The water temperature was between 23 -27 °C. The pH-value of the aerated water was between 6.0 - 8.5 and the total hardness was between 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH. For at least seven days prior to testing the dissolved oxygen was above 80 % saturation. The fish were exposed to light for 12 to 16 hours daily. The fish were fed each day with granular rearing food with approx. 2 % of their body-weight. The food was obtained from a commercial supplier.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Fish were observed at 0, 4, 24, 48, 72 and 96 hours after test start.
- Post exposure observation period:
- None
- Hardness:
- 13°dH corresponding to 232 mg CaCO3/L
- Test temperature:
- 23.4 – 24.1 °C
- pH:
- 7.19 – 7.97
- Dissolved oxygen:
- ≥ 92 % of air saturation pH
- Salinity:
- Not applicable
- Conductivity:
- Not stated
- Nominal and measured concentrations:
- Nominal: 35 mg/L
Measured: 35.0 mg/L - Details on test conditions:
- Test Medium
The test medium was reconstituted test water consisting of analytical grade salts dissolved in purified water with a ratio of Ca:Mg and Na:K of 4:1 and 10:1, respectively, based on molarity, and a conductivity of 710 µS/cm. The pH was between 6.0 and 8.5 and total hardness was 232 mg/L (as CaCO3), corresponding to 13°dH.
Test Units
For the test 2 L glass beakers, filled with 2 L of test solution were used.
Test Conditions
Temperature: 23.4 – 24.1 °C
Oxygen saturation: ≥ 92 % of air saturation
pH-value: 7.19 – 7.97
Hardness: 13°dH corresponding to 232 mg CaCO3/L
Exposure to light: 16 hours daily
Feeding: none
Loading: max. loading of 0.505 g fish/L
Aeration: continuous aeration of the test vessels with a membrane pump using a Pasteur pipette
Assessments
Mortality and Sub-lethal Effects
Fish were considered dead if there was no visible movement (e.g. gill movement), and if touching of the caudal peduncle produced no reaction. Records were made on visible abnormalities as: loss of equilibrium, swimming behaviour, respiratory function, pigmentation and all other observed events. Dead fish were removed if observed and mortality, length and weight was recorded. At termination of the test, all remaining fish were euthanized and all fish were weighed and measured.
Physico-Chemical Assessments
Measurements of temperature, pH-value and oxygen saturation were performed in 24 hour intervals in fresh and aged test solutions. Water hardness of the untreated medium was determined at the beginning of the test. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The LC50 (96 h) of the limit test was determined to be > 35.0 mg/L test item (nominal). The corresponding NOEC (mortality) (96 h) was 35.0 mg/L test item (nominal). No sublethal effects were observed in the control and in the limit test item concentration of 35.0 mg/L after 96 h.
Mortality
No mortality was observed in the control and in the limit test concentration of 35.0 mg/L.
Clinical signs
No sublethal effects were observed in the control and at the limit test concentration of 35.0 mg/L.
Length and weight
All fish were weighed and measured
The average weight of the test organisms was 0.1387 0.0299 g; the average length was 26 +/- 2 mm.
Analytical Results
The content of Guanyl urea phosphate in test solutions was analytically determined to confirm the correct application of the test item. The measured content of Guanyl urea phosphate in the fresh and aged test solution was 103 % of nominal. Since the content of Guanyl urea phosphate in the samples was between 80 and 120 % of nominal, the toxicological endpoints were evaluated using nominal test item concentrations. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not applicable. The measured content of Guanyl Urea Phosphate in the fresh and aged test solution was 103 % of nominal. Since the content of active ingredient in the samples was between 80 and 120 % of nominal, the toxicological endpoints were evaluated using nominal test item concentrations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 (96 h) of the test item was determined to be > 35.0 mg/L test item (nominal). The corresponding NOEC (mortality) (96 h) was 35.0 mg/L test item (nominal). No sublethal effects were observed in the control and the test item concentration of 35.0 mg/L after 96 h. The absence of mortality at the threshold concentration of 35.0 mg/L indicates that fish is not the most sensitive group of test organism and that the LC50 is greater than the limit test concentration.
- Executive summary:
The short term toxicity to fish was determined in accordance with OECD Guideline 203 taking into consideration OECD (2010): No.126 Short Guidance On The Threshold Approach for Acute Fish Toxicity, 2010.
The test item was evaluated in a static limit test, 96 h exposure in treated test medium; 7 organisms ( zebrafish; Danio rerio) for the test concentration and the control were used. The test solution (35 mg/L) was prepared by dilution of the test item in test medium and application of a defined volume of the stock solution to the test vessel. Assessments on effects and mortality after 0, 4, 24, 48, 72 and 96 hours were conducted.
The content of Guanyl Urea Phosphate in test solutions was analytically determined to confirm the correct application of the test item. The measured content of Guanyl Urea Phosphate in the fresh and aged test solution was 103 % of nominal. Since the content of active ingredient in the samples was between 80 and 120 % of nominal, the toxicological endpoints were evaluated using nominal test item concentrations
The LC50 (96 h) of the test item was determined to be > 35.0 mg/L test item (nominal). The corresponding NOEC (mortality) (96 h) was 35.0 mg/L test item (nominal). No sublethal effects were observed in the control and the test item concentration of 35.0 mg/L after 96 h. The absence of mortality at the threshold concentration of 35.0 mg/L indicates that fish is not the most sensitive group of test organism and that the LC50 is greater than the limit test concentration.
Reference
Description of key information
The short term toxicity to fish was determined in accordance with OECD Guideline 203 taking into consideration OECD (2010): No.126 Short Guidance On The Threshold Approach for Acute Fish Toxicity, 2010.
The test item was evaluated in a static limit test, 96 h exposure in treated test medium; 7 organisms ( zebrafish; Danio rerio) for the test concentration and the control were used. The test solution (35 mg/L) was prepared by dilution of the test item in test medium and application of a defined volume of the stock solution to the test vessel. Assessments on effects and mortality after 0, 4, 24, 48, 72 and 96 hours were conducted.
The content of Guanyl Urea Phosphate in test solutions was analytically determined to confirm the correct application of the test item. The measured content of Guanyl Urea Phosphate in the fresh and aged test solution was 103 % of nominal. Since the content of active ingredient in the samples was between 80 and 120 % of nominal, the toxicological endpoints were evaluated using nominal test item concentrations
The LC50 (96 h) of the test item was determined to be > 35.0 mg/L test item (nominal). The corresponding NOEC (mortality) (96 h) was 35.0 mg/L test item (nominal). No sublethal effects were observed in the control and the test item concentration of 35.0 mg/L after 96 h. The absence of mortality at the threshold concentration of 35.0 mg/L indicates that fish is not the most sensitive group of test organism and that the LC50 is greater than the limit test concentration.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 35 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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