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EC number: 207-894-3 | CAS number: 499-83-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pyridine-2,6-dicarboxylic acid
- EC Number:
- 207-894-3
- EC Name:
- Pyridine-2,6-dicarboxylic acid
- Cas Number:
- 499-83-2
- Molecular formula:
- C7H5NO4
- IUPAC Name:
- pyridine-2,6-dicarboxylic acid
- Test material form:
- solid: crystalline
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- A solution containing 100.5 mg/L test item in dilution water was prepared and treated with ultrasonication. In the non-GLP pre-test, pH of the test item solution was 3.5, therefore the pH was adjusted to 7.9 (as in the dilution water) and then the test item solution was used for the test.
This procedure is in agreement with the OECD guidance document no. 23.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species Daphnia magna
Authority STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Selection of the test system was made following the proposal of the guidelines
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.502 mmol/L
- Test temperature:
- 19.8 – 21.6°C
- pH:
- 7.8
- Dissolved oxygen:
- 8.9 mg/L
- Nominal and measured concentrations:
- The measured concentration at the beginning of the test was at 102.39% of the nominal concentration and after 48 hours the measured concentration was at 114.58% of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration.
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
1.1 Biological Results Test Item
The biological results are presented in the following table:
Table 9.3‑a Biological Results Test Item
Parameter |
Value |
24h EC50 |
> 100 mg/L |
48h EC50 |
> 100 mg/L |
48h NOEC |
≥100 mg/L |
48h LOEC |
> 100 mg/L |
2 Validity
¨ Immobilisation
in the controls may not exceed 10 %.
Immobilisation in the controls was 0 %.
¨ The concentration of dissolved oxygen at the end of the test must be at least 3 mg/L. The lowest concentration of dissolved oxygen at the end of the test was 8.9 mg/L.
One valid experiment was performed.
The study was performed as a limit test with 100 mg/L. For the test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.
Toxicity was not observed in the treatment and also none of the animals was immobilised in the blank control.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive controlin a current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined usingHPLC-UV-determination. The measured concentration at the beginning of the test was at 102.39% of the nominal concentration and after 48 hours the measured concentration was at 114.58% of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration.
No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.
The result of the test is considered valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 48h-NOEC≥100 mg/L
48h-LOEC > 100 mg/L
24h-EC50> 100 mg/L
48h-EC50> 100 mg/L - Executive summary:
One valid experiment was performed.
The study was performed as a limit test with 100 mg/L. For the test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised Daphnia were counted.
Toxicity was not observed in the treatment and also none of the animals was immobilised in the blank control.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV-determination. The measured concentration at the beginning of the test was at 102.39% of the nominal concentration and after 48 hours the measured concentration was at 114.58% of the nominal concentration. Therefore, the determination of the biological results was based on the nominal concentration.
The following results were determined for the test itemDipicolinic acid (DPA)(species:Daphnia magna).48h-NOEC≥100 mg/L
48h-LOEC > 100 mg/L
24h-EC50> 100 mg/L
48h-EC50> 100 mg/L
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