Octanoic acid, compound with dicyclohexylamine (1:1)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-11-23 - 1992-11-27

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

not specified

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

49.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

62 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

97.6 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of DCHA against Danio rerio Hamilton Buchanan was determined following EU-Method C.1 resp. OECD Guideline 203.
The following results for the test item DCHA were determined:
96h LC0: 49.2 mg/L
96h LC50: 62 mg/L
96h LC100: 97.6 mg/L

Executive summary:

The acute toxicity of DCHA against Danio rerio Hamilton Buchanan was determined following EU-Method C.1 resp. OECD Guideline 203.

The following results for the test item DCHA were determined:

96h LC0: 49.2 mg/L

96h LC50: 62 mg/L

96h LC100: 97.6 mg/L

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04 Jan- 08 Jan 1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other:

Version / remarks:

Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians (U.S. EPA, 1975)

Deviations:

no

Principles of method if other than guideline:

Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians (U.S. EPA, 1975)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solution was prepared by adding 1.56 g test substance to distilled water and diluting to 1000 mL in a volumetric flask. Subsequently the solution was mixed on a magnetic stirrer for 30 min and then added to each test jar in the required amount. The test medium was also mixed for approximately 2 min.
- Controls: 1
- Evidence of undissolved material: At nominal concentrations of 14, 24 and 40 mg a.s./L the test material was not entirely dissolved.

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

TEST ORGANISM
- Common name: bluegill sunfish
- Source: Commercial fish supplier in Connecticut (USA)
- Length at study initiation: mean: 34 mm (range 25 - 44 mm, n = 30)
- Weight at study initiation: mean: 0.39 g (range: 0.16 - 0.99 g, n = 30)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 14 d
- Acclimation conditions: same as during test
- Type and amount of food: dry pelleted food, ad libitum
- Feeding frequency: daily except during 48 h prior to test
- Health during acclimation: mortality during the 48 h prior to test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

49 mg/L

Test temperature:

22 ± 1 °C

pH:

6.4 - 7.3

Dissolved oxygen:

0.3 - 9.4 mg/L (= 3.4 - 100%)

Nominal and measured concentrations:

nominal: 3.1, 5.2, 8.6, 14, 24 and 40 mg a.s./L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume of test vessel: 19.6 L glass jar containing 15 L test medium
- Aeration: no aeration provided
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark

EFFECT PARAMETERS MEASURED: mortality and sublethal effects every 24 h

Reference substance (positive control):

yes

Remarks:

sodium lauryl sulfate

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% conficdence interval: 17 .- 31 mg a.s./L

Details on results:

- Behavioural abnormalities: fish were lethargic, respiring repidly and/or at water surface
- Mortality of control: no fish died in the control
- Effect concentrations exceeding solubility of substance in test medium: At nominal concentrations of 14, 24 and 40 mg a.s./L the test material was not entirely dissolved.

Results with reference substance (positive control):

LC50: 12 mg sodium lauryl sulfate/L, confidence interval: 10 - 15 mg/L

Validity criteria fulfilled:

yes

Conclusions:

In the key study (1982) the toxicity to Lepomis macrochirus was tested under static conditions according to an EPA-method (1975, Methods for acute toxicity tests with fish macroinvertebrates, and amphibians). Nominal test concentrations were 3.1, 5.2, 8.6, 14, 24 and 40 mg/ L. No analytical monitoring was performed. At all concentrations all fish respired rapidly and the test solution was cloudy. In addition at the three highest concentrations, all fish were lethargic. After incubation time of 96 h, an LC50 of 22 mg/L (nominal) was determined.

Executive summary:

In the key study (1982) the toxicity to Lepomis macrochirus was tested under static conditions according to an EPA-method (1975, Methods for acute toxicity tests with fish macroinvertebrates, and amphibians). Nominal test concentrations were 3.1, 5.2, 8.6, 14, 24 and 40 mg/ L. No analytical monitoring was performed. At all concentrations all fish respired rapidly and the test solution was cloudy. In addition at the three highest concentrations, all fish were lethargic. After incubation time of 96 h, an LC50 of 22 mg/L (nominal) was determined.

Description of key information

DCHA:

The acute toxicity of DCHA against Danio rerio Hamilton Buchanan was determined following EU-Method C.1 resp. OECD Guideline 203.

The following results for the test item DCHA were determined:

96h LC0: 49.2 mg/L

96h LC50: 62 mg/L

96h LC100: 97.6 mg/L

Octanoic acid:

The toxicity of octanoic acid to Lepomis macrochirus was tested under static conditions according to an EPA-method (1975, Methods for acute toxicity tests with fish macroinvertebrates, and amphibians). Nominal test concentrations were 3.1, 5.2, 8.6, 14, 24 and 40 mg/ L. No analytical monitoring was performed. At all concentrations all fish respired rapidly and the test solution was cloudy. In addition at the three highest concentrations, all fish were lethargic. After incubation time of 96 h, an LC50 of 22 mg/L (nominal) was determined.

Since the substance to be registered is Octanoic acid, compound with dicyclohexylamine (1:1) which will dissociate to 1 mol octanoic acid and 1 mol dicyclohexylamine, the lowest LC50 of 22 mg/l for octanoic acid is used for calculating the LC50 for the entire substance considering the molar mass of 325.5 g/mol (44.3% octanoic acid).

Therefore the LC50 for fish for Octanoic acid, compound with dicyclohexylamine (1:1) is 49.66 mg/ml.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

49.66 mg/L

Additional information

Read Across justification:

In aqueous systems Octanoic acid, compound with dicyclohexylamine (1:1) is hydrolytically very unstable so that in aqueous solution a rapid decomposition to the educts can be observed. The substance will rapidly dissociate to octanoic acid and dicyclohexylamine. Therefore Octanoic acid, compound with dicyclohexylamine (1:1) is not stable at “standard” testing conditions representative for human and environmental exposure. Hence, it is fully justified to apply the read-across methodology by use of the respective data from the breakdown/decomposition products to describe the (eco)toxicological behaviour of the substance to be registered.