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EC number: 618-690-2 | CAS number: 90982-32-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- ethyl 2-({[(4-chloro-6-methoxypyrimidin-2-yl)carbamoyl]amino}sulfonyl)benzoate
- EC Number:
- 618-690-2
- Cas Number:
- 90982-32-4
- Molecular formula:
- C15H15ClN4O6S
- IUPAC Name:
- ethyl 2-({[(4-chloro-6-methoxypyrimidin-2-yl)carbamoyl]amino}sulfonyl)benzoate
- Test material form:
- solid: crystalline
- Details on test material:
- Purity: 97.5%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples were collected from each test chamber at test initiation and at 48 and 96 hours (± 1 hour) to measure concentrations of the test substance. The samples were collected at mid-depth and placed in glass vials. Two sets of samples were collected at each interval. One set was processed immediately for analysis.
- Sample storage conditions before analysis: The second set was stored refrigerated for possible future analysis.
Test solutions
- Vehicle:
- yes
- Remarks:
- Filtered saltwater
- Details on test solutions:
- Preparation of Test Concentrations:
The 120 mg a.s./L test solution, which served as a primary stock for the lower test concentration solutions, was prepared by weighing 3.6810 grams of the test substance and adding it to 30 L of filtered saltwater. The 120 mg a.s./L test solution was stirred overnight (~20 hours) with a top-down electric mixer. Aliquots of the 120 mg a.s./L test solution were proportionally diluted with filtered saltwater to prepare four additional test solutions at nominal concentrations of 7.5, 15, 30 and 60 mg a.s./L. The 7.5, 15, 30 and 60 mg a.s./L test solutions were mixed approximately 1-2 minutes with a top-down electric mixer. The solutions were adjusted to 100% active substance based up an active substance content of 97.8% as provided on the test substance label. At test initiation and test termination all test solutions appeared clear and colorless, with no visible precipitate.
Test organisms
- Test organisms (species):
- Cyprinodon variegatus
- Details on test organisms:
- TEST ORGANISM
- Common name: sheepshead minnow
- Strain: Cyprinodon variegatus
- Source: Aquatic Bio Systems, Fort Collins, Colorado
- Age at study initiation: Juveniles
- Weight at termination: 0.12 - 0.26 g
- Loading: 0.090 g fish/L
ACCLIMATION
- Acclimation period: At least 14 days
- Acclimation conditions: Temperature: 21.9 to 23.7°C; pH: 7.6 to 8.3; Dissolved oxygen: 6.6 to 8.1 mg/L (84% of saturation); Salinity: 20 to 21 parts per thousand (‰)
- Type and amount of food during acclimation: Fed a diet of Artemia. The fish were not fed for at least two days prior to the test or during the test.
- Feeding frequency during acclimation: Daily during the holding period
- Health during acclimation: No signs of disease or stress
FEEDING DURING TEST
- Test period: unfed
Study design
- Test type:
- static
- Water media type:
- saltwater
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 21.7 to 23.9°C in test chambers; 22 to 23°C measured continuously in the negative control.
- pH:
- 7.9 to 8.2
- Dissolved oxygen:
- 5.9 to 7.5 mg/L
- Salinity:
- 20‰
- Nominal and measured concentrations:
- Nominal: 7.5, 15, 30, 60 and 120 mg/L
Measured: 7.4, 15, 30, 57 and 120 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 25 L stainless steel aquaria containing 15 L of test solution; depth of the test water in a representative chamber was 18.0 cm.
- Aeration: Unaerated
- No. of organisms per vessel: 7 fish per test chamber
TEST MEDIUM
- Source/preparation of dilution water: Natural seawater collected at Indian River Inlet, Delaware. The freshly-collected seawater was passed through a sand filter to remove particles greater than approximately 25 μm, and pumped into a 37,800 L storage tank. The filtered saltwater then was diluted to a salinity of approximately 20‰ with freshwater from a well and was aerated with spray nozzles. Prior to use, the 20‰ water was filtered to 0.45 μm to remove fine particles.
OTHER TEST CONDITIONS
- Photoperiod: 16 hr light and 8 hr dark including 30 min transitional period preceding and following the 16 hr light interval.
- Light intensity: 426 lux at initiation - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 120 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 120 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Details on results:
- All fish in the negative control group and in all treatment groups appeared normal throughout the test, with no observed mortality or sublethal effects.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 96-Hour LC50: >120 mg/L (Mortality)
96-Hour NOEC: 120 mg/L - Executive summary:
The acute toxicity of the test substance to unfed juvenile sheepshead minnow (Cyprinodon variegatus), a saltwater fish, was determined in an unaerated, static, 96-hour dose response test. The test was conducted in accordance with the Test Guideline OPPTS Number 850.1075, Fish Acute Toxicity Test, Freshwater and Marine. Treatments consisted of a dilution water control and nominal test substance concentrations of 7.5, 15, 30, 60 and 120 mg active substance/L.
One test chamber was maintained in each treatment and control group, with seven sheepshead minnows in each test chamber. Observations of mortality and other signs of toxicity were made approximately 5, 24, 48, 72 and 96 hours after test initiation.
The 96-hour LC50 for Cyprinodon variegatus based on mean, measured concentrations of the test substance and mortality was greater than 120 mg/L.
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