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EC number: 219-376-4 | CAS number: 2426-08-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Repeated dose toxicity: inhalation
Administrative data
- Endpoint:
- chronic toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- reproductive toxicity, other
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Reason / purpose for cross-reference:
- reference to same study
- Principles of method if other than guideline:
- To evaluate the inhalation repeated dose toxicity of the test substance male rats were exposed to 50 7-hour exposures to 4 different vapour concentrations.
- GLP compliance:
- no
- Limit test:
- no
- Species:
- rat
- Strain:
- not specified
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Diablo Laboratories
- Weight at study initiation: 57 - 97 gram before acclimatization, at the start of experiment 90 -120 gram.
- Housing: Two to a cage
- Diet: Standard green feed from Simonsen Laboratories,
- Acclimation period: 2 weeks - Route of administration:
- inhalation: vapour
- Type of inhalation exposure (if applicable):
- whole body
- Vehicle:
- air
- Details on exposure:
- The exposures were made in metal chambers, 200 litres in capacity, and the air flow ranged from 10 to 20 litres per minute, depending on the vapour concentration used. The constant metering device delivered the compound to the evaporator where it was vaporized in the air entering the chamber.
- Details on mating procedure:
- Not applicable
- Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- The vapour concentrations were monitored at intervals ranging from twice daily to once weekly, by analysis of air drawn from the sampling port of each chamber. The vapour was absorbed from the air in pyridine, and reacted with pyridinium chloride; the amount of acid consumed was then measured.
- Duration of treatment / exposure:
- 7 hours
- Frequency of treatment:
- daily except weekends
- Dose / conc.:
- 38 ppm (nominal)
- Dose / conc.:
- 75 ppm (nominal)
- Dose / conc.:
- 150 ppm (nominal)
- Dose / conc.:
- 300 ppm (nominal)
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent vehicle
- Parental animals: Observations and examinations:
- The animals were observed at intervals during exposure as well as before and after placement in the chamber, for signs of toxicity or aberrations from normal behaviour. They were weighed once weekly.
- Postmortem examinations (parental animals):
- At the end of the experimental period the survivors were decapitated under light ether anaesthesia for necropsy. After thorough gross inspection, the lungs, livers, and kidneys of all animals were freed of connective tissue blotted lightly to remove excess moisture and weighted for calculation of organ/bodyweight ratios. Sections of these tissues were preserved for microscopic examination. Sections were also preserved from alternate animals of brain, thyroid, thymus, heart, stomach, intestine, pancreas, adrenal, testis, seminal vesicles, and urinary bladder.
Microscopic examination was made of the tissues of 10 animals in each group, with the exceptions of those exposed to 300 ppm, only five of which survived 50 exposures. Tissues of one animal in this group that died after 40 exposures were included. - Statistics:
- Organ/body weight ratios and percentage weight gains were statistically compared by the Student t-test.
- Clinical signs:
- effects observed, treatment-related
- Description (incidence and severity):
- Animals in the 300 ppm group developed an emaciated appearance, and were ill-kempt in appearance, but showed no overt signs of toxicity. At 150 ppm, surviving animals were without further signs of toxicity except that weight gain was retarded.
- Dermal irritation (if dermal study):
- not examined
- Mortality:
- mortality observed, treatment-related
- Description (incidence):
- At 300 ppm, there were 5 deaths, all occurring between the twentieth and thirty-fifth exposures. At the level of 150 ppm, there was one death after 20 exposures. At 38 and 27 ppm, there were no deaths.
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- A decrease in bodyweight gain was significant (p=0.05) in the 300 ppm group and in the 150 ppm groups. At lower doses there was no significance.
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- Atelectasis was the most common finding, appearing in the lungs of 4 controls and 18 of 35 experimental animals. Lung congestion was noted in 2 controls and 4 experimental animals. These lesions would not appear to be related to the treatment. There were no other findings among controls or animals exposed to 39 ppm.
One animal exposed to 75 ppm had severe pneumonia and slight patchy atrophy of the testis. Five animals exposed to 150 ppm had bronchopneumonia. Three of the five survivors in the 300 ppm group had pneumonia; two of these had atrophic testes, and one had also focal liver necrosis. Both the other animals had atrophic testes and atelectasis of the lungs, and one had focal liver necrosis.
The rat that died after 40 exposures had testicular atrophy, and pneumonia with a foreign-body reaction. - Histopathological findings: neoplastic:
- no effects observed
- Other effects:
- not specified
- Dose descriptor:
- NOAEC
- Effect level:
- 38 ppm (nominal)
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- histopathology: non-neoplastic
- Critical effects observed:
- not specified
- Results for F1 and F2 generation: Not examined
- Examination on pups: Not applicable
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 957
- Report date:
- 1957
Materials and methods
- Principles of method if other than guideline:
- To evaluate the inhalation repeated dose toxicity of the test substance male rats were exposed to 50 7-hour exposures to 4 different vapour concentrations.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Butyl 2,3-epoxypropyl ether
- EC Number:
- 219-376-4
- EC Name:
- Butyl 2,3-epoxypropyl ether
- Cas Number:
- 2426-08-6
- Molecular formula:
- C7H14O2
- IUPAC Name:
- 2-(butoxymethyl)oxirane
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Diablo Laboratories
- Weight at study initiation: 57 - 97 gram before acclimatization, at the start of experiment 90 -120 gram.
- Housing: Two to a cage
- Diet: Standard green feed from Simonsen Laboratories,
- Acclimation period: 2 weeks
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- The exposures were made in metal chambers, 200 litres in capacity, and the air flow ranged from 10 to 20 litres per minute, depending on the vapour concentration used. The constant metering device delivered the compound to the evaporator where it was vaporized in the air entering the chamber.
- Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- The vapour concentrations were monitored at intervals ranging from twice daily to once weekly, by analysis of air drawn from the sampling port of each chamber. The vapour was absorbed from the air in pyridine, and reacted with pyridinium chloride; the amount of acid consumed was then measured.
- Duration of treatment / exposure:
- 7 hours
- Frequency of treatment:
- daily except weekends
Doses / concentrationsopen allclose all
- Dose / conc.:
- 38 ppm (nominal)
- Dose / conc.:
- 75 ppm (nominal)
- Dose / conc.:
- 150 ppm (nominal)
- Dose / conc.:
- 300 ppm (nominal)
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent vehicle
Examinations
- Observations and examinations performed and frequency:
- The animals were observed at intervals during exposure as well as before and after placement in the chamber, for signs of toxicity or aberrations from normal behaviour. They were weighed once weekly.
- Sacrifice and pathology:
- At the end of the experimental period the survivors were decapitated under light ether anaesthesia for necropsy. After thorough gross inspection, the lungs, livers, and kidneys of all animals were freed of connective tissue blotted lightly to remove excess moisture and weighted for calculation of organ/bodyweight ratios. Sections of these tissues were preserved for microscopic examination. Sections were also preserved from alternate animals of brain, thyroid, thymus, heart, stomach, intestine, pancreas, adrenal, testis, seminal vesicles, and urinary bladder.
Microscopic examination was made of the tissues of 10 animals in each group, with the exceptions of those exposed to 300 ppm, only five of which survived 50 exposures. Tissues of one animal in this group that died after 40 exposures were included. - Statistics:
- Organ/body weight rations and percentage weight gains were statistically compared by the Student t-test.
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Description (incidence and severity):
- Animals in the 300 ppm group developed an emaciated appearance, and were ill-kempt in appearance, but showed no overt signs of toxicity. At 150 ppm, surviving animals were without further signs of toxicity except that weight gain was retarded.
- Mortality:
- mortality observed, treatment-related
- Description (incidence):
- At 300 ppm, there were 5 deaths, all occurring between the twentieth and thirty-fifth exposures. At the level of 150 ppm, there was one death after 20 exposures. At 38 and 27 ppm, there were no deaths.
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- A decrease in bodyweight gain was significant (p=0.05) in the 300 ppm group and in the 150 ppm groups. At lower doses there was no significance.
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Description (incidence and severity):
- The only significant difference in organ/body weight ratios occurred in the kidneys and lungs of rats in the 300 ppm group, which were heavier than those of the control group (p=0.05). The record of the organ weights for the 75 ppm group was lost in a laboratory accident, but it may be assumed that there were no differences, since there were none at 150 ppm.
- Gross pathological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- There were few findings at necropsy, other than general decrease in body fat. One rat exposed to 150 ppm had tan discoloration of the thymus, and 2 others had small areas of atelectasis in the lung. One rat exposed to 300 ppm had a consolidated lobe in the lung; one had atelectasis of a lobe; another had very small testes; and another a brown discoloration of the thymus. No abnormalities were seen in the other exposure groups.
- Neuropathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- Atelectasis was the most common finding, appearing in the lungs of 4 controls and 18 of 35 experimental animals. Lung congestion was noted in 2 controls and 4 experimental animals. These lesions would not appear to be related to the treatment. There were no other findings among controls or animals exposed to 39 ppm.
One animal exposed to 75 ppm had severe pneumonia and slight patchy atrophy of the testis. Five animals exposed to 150 ppm had bronchopneumonia. Three of the five survivors in the 300 ppm group had pneumonia; two of these had atrophic testes, and one had also focal liver necrosis. Both the other animals had atrophic testes and atelectasis of the lungs, and one had focal liver necrosis.
The rat that died after 40 exposures had testicular atrophy, and pneumonia with a foreign-body reaction. - Histopathological findings: neoplastic:
- no effects observed
- Other effects:
- not specified
Effect levels
- Key result
- Dose descriptor:
- NOAEC
- Effect level:
- 38 ppm (nominal)
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- histopathology: non-neoplastic
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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