Arctium lappa, ext.

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Arctium lappa, ext.

EC Number:

281-658-8

EC Name:

Arctium lappa, ext.

Cas Number:

84012-13-5

IUPAC Name:

Arctium lappa, ext.

Details on test material:

- Name of test material (as cited in study report): Klettenfruchtextrakt conc.
- Physical state / colour / odour: viscous liquid, golden brown, fruity odour

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: SAG 86.81

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

20 + 1°C

pH:

7.6 - 9.6

Details on test conditions:

TEST SYSTEM:
shaking apparature, 2/s
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

OTHER TEST CONDITIONS
- Photoperiod: 24 h
- Light intensity and quality: daylight lamp, 3300 lux

EFFECT PARAMETERS MEASURED:
Growth determined by cell counting under microscope

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

125.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

472.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Reported statistics and error estimates:

NOEC calculated using TUKEY-test

Any other information on results incl. tables

Results: 

Concentration [mg/L]	Inhibition [%] in 72 h
	Biomass	Growth rate
456.1	99	56
240.1	75	17
126.4	71	8
66.5	58	5
35.0	0	-25
18.4	0	40

 

Growth of control: 27 -fold

pH of control: 7.8 (start); 9.4 (after 72 h)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

In a 72- hour acute toxicity study, the cultures of Desmodesmus subspicatus were exposed to Arctium lappa extract at nominal concentrations of 18.4, 35.0, 66.5, 126.4, 240.1, and 456.1 mg/L} under static conditions in accordance with OECD Guideline 201. The EC₅₀ value based on growth rate was 472.5 mg/L.

This toxicity study is classified as acceptable and satisfies the guideline requirements for a growth inhibition study.

 

Results Synopsis

Test Organism: Desmodesmus subspicatus

Test Type: Static 

72-hr EC₅₀: 472.5 mg/L

Endpoint effected: growth rate