Rifamycin, 3-formyl-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Rifamycin, 3-formyl-

EC Number:

236-311-5

EC Name:

Rifamycin, 3-formyl-

Cas Number:

13292-22-3

Molecular formula:

C38H47NO13

IUPAC Name:

(7S,9E,11S,12R,13S,14R,15R,16R,17S,18S,19E)-26-formyl-2,15,17,27,29-pentahydroxy-11-methoxy-3,7,12,14,16,18,22-heptamethyl-6,23-dioxo-8,30-dioxa-24-azatetracyclo[23.3.1.1⁴,⁷.0⁵,²⁸]triaconta-1,3,5(28),9,19,21,25(29),26-octaen-13-yl acetate

Sampling and analysis

Analytical monitoring:

no

Remarks:

The analytical method validation was conducted under study 3201267, however, the study was stopped and the analytical procedure was not produced. No supporting chemical analysis was conducted during the range-finding test.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna were aged of < 24 hours old.

Study design

Test type:

semi-static

Water media type:

other: Elendt M4

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

No information

Test conditions

Hardness:

Not specified

Test temperature:

19.1 to 21.4°C

pH:

7.26 to 7.94

Dissolved oxygen:

8.44 to 8.92 mg/L

Nominal and measured concentrations:

Nominal: 0.005, 0.05, 0.5, 5, 50 mg/L
Measured: -

Details on test conditions:

Range-finding test

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

>= 0.5 - <= 50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Due to the difficult/ unstable nature of the test substance, it was decided to stop after the range finding test. Determined from the range-finding test and based on nominal 3-formyl rifamicina SV concentrations, the EC50 value was estimated to be between 0.5 and 50 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal 3-formyl rifamicina SV concentrations, the 48-h NOEC was estimated to be 0.005 mg/L and the EC50 value was estimated to be between 0.5 and 50 mg/L.