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EC number: 209-939-2 | CAS number: 598-55-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 15, 1982 to February 5, 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to method comparable to OECD Guideline 471.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Ames et al.: Mutation Research 31(6):347-364, 1975
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl carbamate
- EC Number:
- 209-939-2
- EC Name:
- Methyl carbamate
- Cas Number:
- 598-55-0
- Molecular formula:
- C2H5NO2
- IUPAC Name:
- methyl carbamate
Constituent 1
Method
- Target gene:
- TA 1537- uvrB rfa hisC3076 (Frame-shift)
TA 1538- uvrB rfa hisD3052 (Frame-shift)
TA 98- uvrB rfa hisD3052 (Frame-shift)
TA 1535- uvrB rfa hisG46 (Base-pair substitution)
TA 100- uvrB rfa hisG46 (Base-pair and frame shift)
WP-2 uvrA- uvrA tryp (Base-pair substitution)
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1537
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 1535
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S-9
- Test concentrations with justification for top dose:
- Stock solutions:
0.5, 1.58, 5, 15.8 and 50 mg/mL for plate incorporation tests
0 and 10 mg/mL for disc tests
Tested concentrations
0, 50, 158, 500, 1,581 and 5,000 μg/mL for plate incorporation tests
0 and 1,000 μg/mL for disc tests - Vehicle / solvent:
- - Solvent vehicle: Dimethylsulfoxide (DMSO)
- Source: J. T. Baker Chemical Company, Phillipsburg, NJ
- Lot or control number: 115001106 and 020005286
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
Plate incorporation method and disc method (as impregnation on paper disc)
PLATE INCORPORATION METHOD
Base layer plates containing agar and minimal media were prepared in advance. On the day of the test, 0.1 mL sample at several dose levels was mixed with 0.1 mL bacterial cells, 0.5 mL S-9 mix or buffer, and trace amounts of histidine, biotin and tryptophan. This mixture was added to 2 mL of molten top agar at 45°C and poured on the base layer and allowed to solidify. Revertant colonies were counted after approximately 48 h incubation at 37°C. Three replicate plates were used for each dose level. For solvent controls, 0.1 mL of the solvent DMSO was used in place of test substance. Plates having less than 30 colonies were counted manually; other plates were counted with a New Brunswick Biotran II colony counter or manually.
DISC TEST
The disc test was performed in the same manner as the plate incorporation test except that the test substances were placed on paper discs as opposed to being incorporated in the top agar. For the solvent, 0.1 mL was placed on one-half inch discs. For the test substance, 0.1 mL of the 10 mg/mL concentration was placed on one-half inch discs. For the positive control mutagen, 10 μl of the appropriate concentration was placed on one-fourth inch discs. One disc was used per plate.
SOURCE:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100: Dr. B. N. Ames, University of California, Berkeley, California.
Escherichia coli WP-2 uvrA: Dr. B. Bridges, MRC Cell Mutation Unit, University of Sussex, Falmar, Brighton, England. - Evaluation criteria:
- In the plate incorporation assay, if the mean number of revertants on plates at a given concentration is no more than twice the mean number on the solvent control plates, the test compound is considered non-mutagenic.
In the disc test, the presence of a ring of revertants around the disc indicates the test compound is mutagenic.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
No mutagenic effect of test substance was observed either in the presence or absence of metabolic activation system by plate incorporation and disc method. - Executive summary:
An in vitro bacterial reverse mutation assay was performed to test the potential of the test substance to cause gene mutation by method comparable to OECD Guideline 471. The assay was performed by 2 methods (plate incorporation and disc test) with 6 bacterial strains, Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538, and Escherichia coli WP-2 uvrA, in the presence and absence of Aroclor 1254-induced rat liver S-9. The test substance was tested at concentrations of 0, 50, 158, 500, 1,581 and 5,000 μg/mL for plate incorporation tests with three replicate plates per concentration and at concentrations of 0 and 1,000 μg/mL for disc tests with one replicate per concentration. Concurrent positive control mutagens and solvent controls were also used. Revertant colonies were counted after approximately 48 h incubation at 37°C. Plates having less than 30 colonies were counted manually; other plates were counted with a New Brunswick Biotran II colony counter or manually. The WP-2 uvrA was contaminated and therefore test was repeated for all strains. No dose level produced the mean number of revertants on plates more than twice the mean number on the solvent control plates in the plate incorporation assay. In addition, no plate containing a disc impregnated with test substance showed a ring of revertants around the disc. Positive control gave positive results, hence meeting validity criteria. Under the study conditions, no mutagenic effect of test substance was observed either in the presence or absence of metabolic activation system by plate incorporation and disc method (Allen JS, 1982).
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