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Diss Factsheets
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EC number: 203-306-4 | CAS number: 105-54-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from authoritative database.
Data source
Referenceopen allclose all
- Reference Type:
- other: authoritative database
- Title:
- Acute Inhalation toxicity - LC50
- Author:
- U.S. National Library of Medicine
- Year:
- 2 012
- Bibliographic source:
- HSDB
- Reference Type:
- other: study report
- Title:
- SIDS INITIAL ASSESSMENT PROFILE
- Author:
- US/ICCA
- Year:
- 2 006
- Bibliographic source:
- SIAM 23, 17-20 October 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Acute Inhalation toxicity of the given test chemical in rat.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
Test material
- Reference substance name:
- Ethyl cyanoacetate
- EC Number:
- 203-309-0
- EC Name:
- Ethyl cyanoacetate
- Cas Number:
- 105-56-6
- Molecular formula:
- C5H7NO2
- IUPAC Name:
- Ethyl cyanoacetate
- Details on test material:
- - IUPAC Name: Ethyl cyanoacetate
- Common Name: ECYA
- InChI: 1S/C5H7NO2/c1-2-8-5(7)3-4-6/h2-3H2,1H3
- Smiles: C(CC#N)(OCC)=O
- Molecular formula:C5H7NO2
- Molecular weight :113.1153 g/mol
- Substance type:Organic
- Physical state:Liquid - colorless mild pleasant odour
- Purity: 99.7%
- Impurities (identity and concentrations):0.3%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River UK
- Age at study initiation: 6 to 8 weeks
- Weight at study initiation: ca. 200 g
- Housing: Animals were kept in cages.
- Diet (e.g. ad libitum): Food was provided
- Water (e.g. ad libitum): water was provieded
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- whole body
- Vehicle:
- clean air
- Mass median aerodynamic diameter (MMAD):
- 5.8 µm
- Geometric standard deviation (GSD):
- 2.23
- Remark on MMAD/GSD:
- Sampling with a Marple cascade impactor 35 minutes after the start of exposure.
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: exposure chamber
- Exposure chamber volume: 120 L
- Method of holding animals in test chamber: Animals were held in 10 separate animal compartments.
- Source and rate of air: Respirable aerosol in clean dried air produced by aerosol generator (equilibration period 9 min),
- Temperature, humidity, pressure in air chamber: supply pressure 15 l/min, flow rate in the exposure chamber: 0.8 ml/min per pump, total flow rate 1.6 ml/min.
TEST ATMOSPHERE (if not tabulated)
- Particle size distribution:
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): mass median aerodynamic diameter (MMAD): 5.8 µm, geometric standard deviation: 2.23, 59.5% in the respirable range (< 7 µm).
CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration: Analytical determination, sampling through a gas absorption trap, 3 samples were taken during the exposure period - Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 1 h
- Remarks on duration:
- not specified
- Concentrations:
- 7.38 mg/l +- 0.131 (nominal 58 mg/l or 7380 mg/m3)
- No. of animals per sex per dose:
- 5 males, 5 females per group
- Control animals:
- yes
- Remarks:
- clean air only
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Clinical signs: continuously during exposure, twice daily during the observation period.
Body weights: daily. Food and water consumption per cage.
- Necropsy of survivors performed: yes
- Other examinations performed: Macroscopic organ examination at necropsy, lung weights. - Statistics:
- not specified
Results and discussion
- Preliminary study:
- not specified
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 7 380 mg/m³ air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 1 h
- Remarks on result:
- other: No mortality was observed
- Mortality:
- No mortality was observed at 7380 mg/m3.
- Clinical signs:
- other: During observation period, immediately after removal from the exposure chamber: exaggerated respiratory movements and matted fur (all rats), wet fur around the snout and jaws (2 males) and clear discharge from the eyes (3 females), brown staining around t
- Body weight:
- Slightly reduced body weight and food consumption on day 1, thereafter comparable to control. Water consumption not affected.
- Gross pathology:
- At necropsy there were no macroscopic abnormalities in the rats.
- Other findings:
- Lung to body weight ratio were comparable between test and control animals.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- The acute inhalation toxicity dose (LC50) value was considered to be >7380 mg/m3, when 10 male and female Sprague-Dawley rats were treated with test chemical via inhalation route by aerosol to whole body exposure for 1 hour.
- Executive summary:
Acute inhalation toxicity study of test chemical was conducted in 10 male and female Sprague-Dawley rats at the dose concentration of 7380 mg/m3 (7.38 mg/l). The exposure was given towhole body, respirable aerosol in clean dried air produced by aerosol generator (equilibration period 9 min), and exposure chamber 120 l with 10 separate animal compartments. The control animals received clean air only. Animals were observed for - clinical signs: continuously during exposure, twice daily during the observation period; body weights: daily; Food and water consumption per cage. Necropsy of survivors was performed. Macroscopic organ examination at necropsy and lung weights was done. No mortality was observed at 7380 mg/m3.During observation period, immediately after removal from the exposure chamber: exaggerated respiratory movements and matted fur (all rats), wet fur around the snout and jaws (2 males) and clear discharge from the eyes (3 females), brown staining around the snout and jaws (1 female) were observed. All symptoms were reversible within 24 hours. Slightly reduced body weight and food consumption on day 1, thereafter comparable to control. Water consumption not affected. At necropsy there were no macroscopic abnormalities in the rats. Lung to body weight ratio were comparable between test and control animals. Therefore, LC50 value was considered to be >7380 mg/m3, when 10 male and female Sprague-Dawley rats were treated with test chemical via inhalation route by aerosol to whole body exposure for 1 hour.
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