Dimethyl sulphone

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Data is from peer-reviewed journal

Data source

Reference

Reference Type:

publication

Title:

Evaluation of Genotoxicity on Plant-Derived Dietary Sulfur

Author:

LEE, YOON-IK

Year:

2006

Bibliographic source:

J. Microbial. Biotechnol.

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

The in vitro chromosome aberration test was carried out using Chinese Hamster Lung (CHL) cells to evaluate genetic potential of substance dimethyl sulphone (Methylsulfonylmethane).

GLP compliance:

no

Type of assay:

in vitro mammalian chromosome aberration test

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl sulphone

EC Number:

200-665-9

EC Name:

Dimethyl sulphone

Cas Number:

67-71-0

Molecular formula:

C2H6O2S

IUPAC Name:

dimethyl sulphone

Method

Target gene:

Chinese Hamster Lung (CHL) cells

Species / strain

Species / strain / cell type:

mammalian cell line, other: Chinese Hamster Lung (CHL) cells

Details on mammalian cell type (if applicable):

Details on mammalian cell line
- Type and identity of media: The cell were cultured in Eagles minimum essential medium (EMEM) supplemented with 10% fetal bovine serum. The cell were incubated in a 95% air and 5% CO₂ atmosphere at 37°C. The cell were plated at a density of 2.5ẋ10⁵ cells on 6cm plates and grown for 22h, and then colcemid (0.25g/ml) was added 2hr before harvest.
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: No data available
- Periodically checked for karyotype stability: No data available
- Periodically "cleansed" against high spontaneous background: No data available

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

rat liver S9 fraction

Test concentrations with justification for top dose:

0, 5, 2.5, 1.25 mg/ml

Vehicle / solvent:

Vehicle
- Vehicle(s)/solvent(s) used: No data available
- Justification for choice of solvent/vehicle: No data available

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

benzo(a)pyrene

methylmethanesulfonate

Details on test system and experimental conditions:

METHOD OF APPLICATION: plate incorporation

DURATION
- Preincubation period: No data available
- Exposure duration: 24hr
- Expression time (cells in growth medium): 22 hr
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): 24 hr

SELECTION AGENT (mutation assays): No data available

SPINDLE INHIBITOR (cytogenetic assays): No data available

STAIN (for cytogenetic assays): No data available

NUMBER OF REPLICATIONS: No data available

NUMBER OF CELLS EVALUATED: No data available

DETERMINATION OF CYTOTOXICITY
- Method: No data available

OTHER EXAMINATIONS
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available

Rationale for test conditions:

No data

Evaluation criteria:

No data

Statistics:

No data

Results and discussion

Test results

Key result

Species / strain:

mammalian cell line, other: Chinese Hamster Lung (CHL) cells

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data available
- Effects of osmolality: No data available
- Evaporation from medium: No data available
- Water solubility: No data available
- Precipitation: No data available
- Other confounding effects: No data available

RANGE-FINDING/SCREENING STUDIES: No data available

COMPARISON WITH HISTORICAL CONTROL DATA: No data available

ADDITIONAL INFORMATION ON CYTOTOXICITY: No data available

Remarks on result:

other: no mutagenic potential

Applicant's summary and conclusion

Conclusions:

In the in vitro chromosome aberration test using Chinese Hamster Lung (CHL) cells, no abberation effects were seen.Therefore, the test substance is considered to be non mutagenic in Chinese Hamster Lung (CHL) cells.

Executive summary:

The in vitro chromosome aberration test was carried out using Chinese Hamster Lung (CHL) cells to evaluate the potential of test substance damaging chromosome. The cell were exposed to test substance at concentration 0, 5, 2.5, 1.25 mg/ml for 24 hr. Methylmethane sulfon ate (MMS)(0.02 mg/ml) and Benzo[a]pyrene (B[a]P) (0.02mg/ml) used as positive control substances. After treatment with test substance , no significant increase in the number of aberrant cells was observed. In presence or absence of S9 mix, the chromosome aberration produced by treatment with Methylsulfon ylmethane was less than 2 %. These results indicate that methylsulfonylmethane did not increase chromosome aberration as compared with the negative control. Therefore, the test substance is considered to be non genotoxic.