Registration Dossier
Registration Dossier
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EC number: 268-667-2 | CAS number: 68133-24-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- inherent biodegradability
- Type of information:
- other: read across
- Adequacy of study:
- key study
- Study period:
- 20 September 1995 to 18 October 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: study according to the guideline under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- yes
- Remarks:
- single samples per test vessel were analysed for DOC
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hexasodium 4,4'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate]
- EC Number:
- 269-284-3
- EC Name:
- Hexasodium 4,4'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate]
- Cas Number:
- 68214-04-0
- Molecular formula:
- C44H24Cl2N14Na6O20S6
- IUPAC Name:
- hexasodium 4,4'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate]
- Test material form:
- solid
Constituent 1
Study design
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum: Activated sludge of a communal sewage treatment plant Ara Reinach collected on 19.09.95
- Storage conditions: no data
- Storage length: one day
- Preparation of inoculum for exposure: no data
- Pretreatment: none reported
- Concentration of sludge: 530 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 593 mg/L
- Based on:
- test mat.
- Initial conc.:
- 150 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: accoding to the guideline
- Additional substrate: none
- Solubilising agent : none
- Test temperature: 21.1-22.8 °C
- pH: 6.8-7.5
- pH adjusted: no data
- Aeration of dilution water: yes (O2 concentration 6.2-8.3 mg/L)
- Continuous darkness: no, indirect daylight
TEST SYSTEM
- Culturing apparatus: 2 L vessel containing test substance, activated sludge and mineral medium (agitated)
- Number of culture flasks/concentration: 2 for test substance, one for abiotic control, one for reference substance
- Method used to create aerobic conditions: no data
- Measuring equipment: TOC/DOC Analyzer 500
SAMPLING
- Sampling frequency: at start, after 3 hours and on day 1, 2, 5, 7, 9, 12, 14, 16, 19, 21, 23, 27 and 28
- Sampling method: no data
- Sample storage before analysis: no data
CONTROL AND BLANK SYSTEM
- Inoculum blank: included
- Toxicity control: included
STATISTICAL METHODS: N/A
Reference substance
- Reference substance:
- diethylene glycol
Results and discussion
- Preliminary study:
- Absorption (corrected for blank): 0%
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- results are average of 2 tests running in parallel
BOD5 / COD results
- Results with reference substance:
- The biodegradation, corrected by the blank control and measured as DOC (mg/l) is: 98 % on 8th day (average of 2 tests running in parallel).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The substance is not inherently biodegradable
- Executive summary:
In a Zahn-Wellens test the substance did not show any biodegradation over a 28 day period. Therefore it is concluded that the substance is not inherently biodegradable.
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