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Diss Factsheets
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EC number: 457-630-8 | CAS number: 2138836-26-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan. 10, 2005 and Jan. 14, 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- Some test fish had a bit larger size than the one recommended in the test guideline for the zebra fish. This deviatiation was not regarded to be relevant to the results.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: at 0, 24, 48, 72 and 96 h
- Storage: Routinely, the samples were analysed immediately. Only in exceptional cases, they were stored overnight deeply frozen and protected from light. - Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: Aqua K16Ger (Germany)
- Length at study initiation: 3.48 cm (S.D. = 0.14 cm, n = 20)
- Feeding during test: no feeding during the exposure period
ACCLIMATION
- Acclimation period: Dec. 06, 2004 to Jan. 09, 2005 (35 d)
- Acclimation conditions: Temperature 0 - 24°C, dissolved oxygen > 5 mg/L
- Type and amount of food: Commercial fish food. Discontinued 24 h prior to test.
- Feeding frequency: daily
- Health during acclimation (any mortality observed): <5 % - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 291 mg/L CaCO3
- Test temperature:
- 21.1 - 22.0°C
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- 7.9 - 9.0 mg/L (89 - 101%)
- Nominal and measured concentrations:
- 0, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria holdiug 5 L of test media
- Aeration: gentle aeration via narrow glass tubes
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel:
- Biomass loading rate: 0.95 g bw (wet weight)/L
OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : observations on mortality at 2, 24, 48, 72 and 96 h and subjective assessments on type and incidence of sub-lethal effects compared with controI fish.
TEST CONCENTRATIONS
- Test concentrations: 0 and 100 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Recovery rates correspond to 99.7% of nominal values at 0 h, to 100.5% of nominal values at 24 h, to 100.3% of nominal values at 48 h, to 98.9% of nominal values at 72 h and 98.6% of nominal values at 96 h, respectively.
No deaths or abnormalities occurred in control or test groups during the exposure period.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the 96 h LC50 and NOEC of the test substance were found to be >100 mg/L and 100 mg/L (nominal), respectively.
- Executive summary:
A study was conducted to determine the acute toxicity of the test substance to zebra fish (Danio rario) according to EU method C.1 and in compliance with GLP.
The study consisted of two groups of ten fish exposed to at the concentrations of 0 mg/L (control) and 100 mg/L of test substance. Observations were made for the mortality and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 h of exposure. The analytical concentration of the test substance was measured by HPLC and was found to be 99.7 mg/L at 0 h, and 98.6 mg/L at 96 h. No mortality was observed in the control or test substance treated groups.
Under the test conditions, the 96 h LC50 and NOEC of the test substance were found to be >100 mg/L and 100 mg/L (nominal), respectively.
Reference
Description of key information
The 96-h LC50 and NOEC of the test substance were found to be >100 and 100 mg/L (nominal ), respectively.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
A study was conducted to determine the acute toxicity of the test substance to zebra fish (Danio rario) according to EU method C.1, in compliance with GLP. The study consisted of two groups of ten fish exposed to concentrations of 0 (control) and 100 mg/L of test substance. Observations were made for mortality and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 h. The analytical concentration of the test substance was measured by HPLC and was found to be 99.7 mg/L at 0 h and 98.6 mg/L at 96 h. No mortality was observed in the control or test substance treated groups. Under the test conditions, the 96 h LC50 and NOEC were found to be >100 and 100 mg/L (nominal concentrations), respectively (Bruns, 2005b).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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