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EC number: 219-755-4 | CAS number: 2524-04-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1973
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No guideline available at the time the study was conducted, but performed comparable to current guidelines.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 973
- Report date:
- 1973
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- Animals were treated at different volumes of the test substance; no individual data presented in report
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory by the time the study was conducted.
- Test type:
- other: non-guideline, but comparable to standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- O,O-diethyl phosphorochloridothioate
- EC Number:
- 219-755-4
- EC Name:
- O,O-diethyl phosphorochloridothioate
- Cas Number:
- 2524-04-1
- Molecular formula:
- C4H10ClO2PS
- IUPAC Name:
- O,O-diethyl chlorophosphonothioate
- Details on test material:
- - Name of test material (as cited in study report): EP2
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: CFLP
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 16-23 g
- Fasting period before study: overnight
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: aqueous solution containing 5% Tween 80
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 5%
- Amount of vehicle (if gavage): 0.4, 0.64, 1, 1.6 or 2.5 mL per kg bw
MAXIMUM DOSE VOLUME APPLIED: 2.5 mL
DOSAGE PREPARATION (if unusual):
The test substance was prepared as a 5% emulsion in 5% Tween 80 (aqueous solution).
- Rationale for the selection of the starting dose:
Doses for the full scale test were selected based on the results of a preliminary range finding study. - Doses:
- full scale test: 0, 0.4, 0.64, 1, 1.6 or 2.5 mL/kg bw of a 5% solution of the test substance, corresponding to 0, 476, 762, 1190, 1380, or 2975 mg/kg bw.
(dose-range finder: 0, 0.4, 1 or 2 mL/kg bw of a 5% solution of the test substance, corresponding to 0, 476, 1190, or 2380 mg/kg bw) - No. of animals per sex per dose:
- full scale test: 5/sex/dose
(dose-range finder: 2/sex/dose) - Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observation for mortality every hour during the first 24 hours, and daily afterwards; weight was taken at the day of dosing and then once per week
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- Not applicable
Results and discussion
- Preliminary study:
- The results of the dose range finding study indicated that the median lethal oral dose (LD50) was in the range of 0.4 - 2mL/kg bw (corresponding to 476 - 2380 g/kg bw).
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 714 mg/kg bw
- Based on:
- not specified
- 95% CL:
- > 512 - < 1 059
- Mortality:
- Deaths occured between one and 24 hours after dosing.
- Clinical signs:
- other: Signs of reaction to treatment, observed within a few hours after dosing included piloerection and lethargy in all animals. This was accompanied by body tremors in mice treated at dosages greater than 0.4 mL/kg bw, togehter with excess salivation in anima
- Gross pathology:
- Autopsy of the animals found dead after treatment revealed darkening of the liver and kidneys and injection of the intestinal blood vessels. Autopsy findings of animals surviving treatment were normal.
Any other information on results incl. tables
Mortality ratio and group mean bodyweight of mice dosed orally at EP2 - Results of the full scale test:
Sex |
Dosage [mL/kg bw] |
Bodyweight [g] at |
Mortality ratio (No. dead / No. dosed) |
Time of death after dosing [h] |
||
Dosing |
Week 1 |
Week 2 |
||||
Male |
0 |
20 |
26 |
29 |
0 / 5 |
-- |
0.4 |
21 |
28 |
32 |
0 / 5 |
-- |
|
0.64 |
22 |
24 |
28 |
2 / 5 |
<23 |
|
1 |
21 |
26 |
31 |
4 / 5 |
<7 |
|
1.6 |
20 |
-- |
-- |
5 / 5 |
<5 |
|
2.5 |
20 |
-- |
-- |
5 / 5 |
<2 |
|
|
||||||
Female |
0 |
20 |
27 |
32 |
0 / 5 |
-- |
0.4 |
21 |
28 |
31 |
1 / 5 |
<5 |
|
0.64 |
20 |
-- |
-- |
5 / 5 |
<5 |
|
1 |
21 |
24 |
29 |
4 / 5 |
<7 |
|
1.6 |
20 |
-- |
-- |
5 / 5 |
<5 |
|
2.5 |
20 |
-- |
-- |
5 / 5 |
<2 |
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- The acute median lethal dose (LD50) and its 95% confidence limits to mice of EP2 were calculated to be 0.6 (0.43 - 0.89) mL/kg bw, corresponding to 0.714(512 - 1059)mg/kg bw.
- Executive summary:
The study was conducted in order to assess the acute oral toxicity of EP2 (O,O-diethyl chlorothiophosphate).
Therefore, mice were treated at a single dose of 0 - 2.5 mL of a 5% solution of the test substance in an aqueous solution containing 5% Tween 80 (dosage sorresponds to 0 - 2975 mg/kg bw).
During a 14 day post-exposure period, mortalities and signs of toxicity were recorded. All mice that died and those that survived the observation period were subjected to autopsy, in an attempt to identify the target organs.
Signs of reaction to treatment included piloerection and lethargy (all animals), body tremors (>0.4 mL/kg bw) and excess salivation (0.64 and 1 mL/kg bw). Death occured between one and 24 hours after test substance administration. Autopsy revealed darkening of liver and kidneys and injection of the intestinal blood vessels. Recovery of survivors was complete within one day of treatment and no adverse findings were made during autopsy. Bodyweights were not affected by treatment, except in one female which survived treatment at 1 mL/kg bw, where reduced bodyweight gain was noted during the first week after exposure, but not during week 2 after dosage.
Based on the results, the LD50 was calculated to be 0.6 mL/kg bw (corresponding to 714 mg/kg bw).
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