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Diss Factsheets
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EC number: 309-849-4 | CAS number: 101316-44-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: study documented in sufficient detail
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- A test of the equipotency of internal burdens of nine narcotic chemicals using Daphnia magna.
- Author:
- Pawlisz AV, Peters RH
- Year:
- 1 993
- Bibliographic source:
- Environ Sci Technol 27: 2801-2806
- Reference Type:
- publication
- Title:
- Effects of sublethal exposure on lethal body burdens of narcotic organic chemicals in Daphnia magna.
- Author:
- Pawlisz AV, Peters RH
- Year:
- 1 995
- Bibliographic source:
- Environ Sci Technol 29: 613-621
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- no guideline available
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Excessive number of animals per concentration and per test volume. Mortality rather than immobility taken as endpoint.
- Principles of method if other than guideline:
- Short-term LC50 test with Daphnia magna exposed to acetophenone for 48 h.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Acetophenone
- EC Number:
- 202-708-7
- EC Name:
- Acetophenone
- Cas Number:
- 98-86-2
- Molecular formula:
- C8H8O
- IUPAC Name:
- 1-phenylethanone
- Details on test material:
- - Name of test material (as cited in study report): acetophenone
- Substance type: aromatic ketone
- Physical state: liquid (mp: ca. 20°C)
- Analytical purity: >98%
- Impurities (identity and concentrations): not reported
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: not reported
- Sampling method: Acetophenone (radiolabeled) concentration was checked by placing an aliquot of the test solution in a scintillation vial
with Ready Protein scintillation fluid, the measured radioactivity was divided by specfic activity to yield the chemical concentration in mmol/L.
- Sample storage conditions before analysis: not reported
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: An aliquot of the chemical was introduced in a volumetric flask containing standard reconstituted water according to APHA 1989.
- Controls: 2 controls (test substance omitted) performed
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not used
- Evidence of undissolved material (e.g. precipitate, surface film, etc): acetophenone is soluble in water (ws: ca. 6 g/L)
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (parthenogenic female)
- Strain: not reported
- Source: Biology Department, McGill University 1205 Dr. Penfield Avenue, Montreal, Quebec, H3A 1B1 Canada
- Age at study initiation (mean and range, SD): not reported
- Length at study initiation (length definition, mean, range and SD): 0.4-3.3 mm
- Method of breeding: ca. 75 parthenogenic female were kept in glass beakers (1-L) containing standard reconsituted water acc. to APHA 1989
and under constant environmental conditions (photoperiod: 16 h light/8 h dark, temperature: 20°C). The animals were fed daily,
and each beaker received enough algal suspension to keep the culture water slightly green.
- Feeding during test: no
ACCLIMATION: not reported in respect to the determination of the LC50
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 23°C
- Nominal and measured concentrations:
- nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: eight 20-mL screw-top vials
- Type (delete if not applicable): closed (vials were closed with aluminium-lined screw caps
- Material, size, headspace, fill volume: not reported, 20 mL, 2 mL, 18 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): static test
- No. of organisms per vessel: 15
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): vehicle not used
- Biomass loading rate: 15 / 18 mL = 0.8 daphnia per mL test medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Standard reconstituted water acc. to APHA 1989
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: 16 h light/8h dark (conditions of the stock culture; for the test performance no deviations from these conditions reported)
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not reported
- Range finding study: not reported
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 528 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- In a static test, a 48 h LC50=528 mg/L (4.4 mmol/L; nominal) was determined.
Any other information on results incl. tables
In a static test, a 48 h LC50=528 mg/L (4.4 mmol/L; nominal) was determined.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Acetophenone shows a low acute toxicity towards Daphnia magna (48 h LC50=528 mg/L, nominal).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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