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Reaction mass of Cobaltate(3-), bis[2-[[[3-[2-[1-[[(2-chlorophenyl)amino]carbonyl]-2-(oxo-κO)propyl]diazenyl-κN1]-4-(hydroxy-κO)phenyl]sulfonyl]amino]benzoato(3-)]-, sodium (1:3) and tetrasodium bis[2-[[[3-[[1-[(2-chloroanilino)carbonyl]-2-oxopropyl]azo]-4-hydroxyphenyl]sulphonyl]amino]benzoato(3-)]cobaltate(4-)
EC number: 941-792-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Test substance: FAT 20049/G
Batch no: EN 007379.71
Purity: ca. 65 %
Appearance: yellow powder
Solubility: 100 g/l
Storage: Room temperature
Expiration date: 03/2003 - Analytical monitoring:
- not specified
- Details on sampling:
- no data
- Vehicle:
- yes
- Details on test solutions:
- Stock solution and test concentrations:
- Stock solution: 1000 mg test substance was dissolved in 200 mL dechlorinated tap water, was homogenized for 10 minutes by ultrasonic treatment and then added up to 1 L. For the preparation of the test concentrations the stock solution was mixed.
- Test concentrations: The nominal test concentrations were selected as 0.04, 0.08, 0.18, 0.40, 0.88, 1.9 and 4.3 mg/L. Additionally a blank control without test substance was tested. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- The test organism was the species Zebra fish (Brachydanio rerio) supplied by Charles River Aquatics, Someren, Netherlands. The quality of the fish was checked with an acclimatization time of 61 d in dechlorinated tap water. For each concentration, 7 fish were used. The fish were not fed during the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- no data
- Hardness:
- 142 mg CaCO3/L
- Test temperature:
- 23 °C
- pH:
- 7.9 - 8.4
- Dissolved oxygen:
- 95 - 98 %
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 0, 0.04, 0.08, 0.18, 0.40, 0.88, 1.9 and 4.3 mg/L
- Details on test conditions:
- Light: Daylight
Apparatus: Static test in 5 L glass aquaria with a test volume of 3 L, slowly aerated. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.08 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.052 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0.31-0.94
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 0.33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- ca. 1.24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- In the control and in the test concentration up to 0.08 mg/L, no mortality and no abnormal behaviour of the test fish were observed over the test period. Therefore, the NOEC and the LC0 value were determined to be 0.08 mg/L. The LC100 value was determined to be 1.9 mg/L nominal.
- Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- The LC50 values were calculated with the Probit method.
- Sublethal observations / clinical signs:
Validity of the test
The test was considered valid because
- no fish was dead in the control at the end of the test
- the oxygen concentration throughout the test was >60 % of the air saturation value.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h LC50 was 0.52 mg/L (i.e. ca. 0.33 mg a.i./L) and the 96 h LC100 was 1.9 mg/L (i.e. ca. 1.24 mg a.i./L).
- Executive summary:
A study was conducted to determine the short term toxicity (LC50) of the test substance (at ca. 65 % purity) to Zebra fish (Brachydanio rerio) according to OECD Guideline 203 and EU Method C.1 in compliance with GLP. Groups of 7 fish were exposed at nominal concentrations of 0, 0.04, 0.08, 0.18, 0.40, 0.88, 1.9 and 4.3 mg/L for 96 h. Mortality and behaviour were recorded at 24, 48, 72 and 96 h. No alteration of the test substance in the media was observed throughout the study. There was no mortality and no abnormal behaviour at concentrations up to 0.08 mg/L. Mortality and moderate symptoms were observed at all other concentration. All fishes were found dead at 1.9 mg/L concentration. Under the test conditions, the 96 h NOEC (LC0) to Zebra fish (Brachydanio rerio) was 0.08 mg/L (i.e. ca. 0.052 mg a.i./L). The 96 h LC50 was 0.52 mg/L (i.e. ca. 0.33 mg a.i./L) and the 96 h LC100 was 1.9 mg/L (i.e. ca. 1.24 mg a.i./L).
Reference
Description of key information
Several studies evaluating toxicity potential of Acid Yellow 220 are available. The study performed in1998 has been selected as key study and was conducted to determine the short-term toxicity (LC50) of the test substance (at ca. 65 % purity) to Zebra fish (Brachydanio rerio) according to OECD Guideline 203 and EU Method C.1 in compliance with GLP. Under the test conditions, the 96 h NOEC (LC0) to Zebra fish (Brachydanio rerio) was 0.08 mg/L (i.e. ca. 0.052 mg a.i./L). The 96 h LC50 was 0.52 mg/L (i.e. ca. 0.33 mg a.i./L) and the 96 h LC100 was 1.9 mg/L (i.e. ca. 1.24 mg a.i./L).
The outcomes of other studies available are presented below:
FAT 20049/E - 96h LC50: 15.7 mg/L
FAT 20049/D - 96h LC50: 13 mg/L
FAT 20049/C - 48h TL50: 0.63 mg/L and 6.3 mg/L
FAT 20049/B - 48h TL50: 0.63 mg/L
FAT 20049/A - 48h TL50: 4.4 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.33 mg/L
Additional information
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