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EC number: 274-386-6 | CAS number: 70209-87-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- yes
- Remarks:
- (observation period was up to 7 d instead of 21 d; eyes were not examined at 1 h after test substance application; clinical observations for systemic effects was not done)
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Chromate(2-), [2,4-dihydro-4-[(2-hydroxy-4-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)]-, disodium
- EC Number:
- 274-386-6
- EC Name:
- Chromate(2-), [2,4-dihydro-4-[(2-hydroxy-4-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)]-, disodium
- Cas Number:
- 70209-87-9
- Molecular formula:
- C32H21CrN10Na2O11S
- IUPAC Name:
- Disodium-[2,4-dihydro-4-[(2-hydroxy-4-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)]-chromate(2-)
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): FAT 20060/B
- Substance type: Dye
- Analytical purity: Approx. 82%
- Lot/batch No.: EN 78708
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Himalayan
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 1.5 to 2 kg
- Housing: The rabbits were caged singly in metal cages
- Diet: Standard rabbit food - NAFAG, Gossau SG (ad libitum)
- Water: ad libitum
- Acclimation period: 4 d
ENVIRONMENTAL CONDITIONS
- Temperature: 20±1 °C.
- Humidity: 55±5 %
- Photoperiod: 10 h (light cycle day)
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: the second eye on the treated animals served as control
- Amount / concentration applied:
- - Amount applied: 100 mg
- Concentration: Undiluted - Duration of treatment / exposure:
- Unwashed eye: Duration of treatment same as observation period
Washed eye: 30 seconds - Observation period (in vivo):
- The eyes were examined for any ocular reaction approximately 1, 2, 3, 4 and 7 d (i.e., 24, 48, 72, 96 and 168 h) after the treatment
- Number of animals or in vitro replicates:
- 6 (i.e., 3 males and 3 females)
- Details on study design:
- PROCEDURE:
-The test was carried out in accordance with the procedure set out in the Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics (1959) of the US Association of Food and Drug Officials (AFDO) which is similar to OECD Guideline 405.
-Only rabbits with normal ophthalmic findings were used for the test.
-The test substance in an amount of 100 mg was inserted into the conjunctival sac of the left eye of the rabbits and the eyelids were gently closed for one second. The right eye was not treated and served as an untreated control.
-In 3 of the 6 rabbits approximately 30 seconds after treatment the treated eye was flushed with 10 mL of lukewarm water.
-The eye irritation was evaluated with a slit-lamp and was scored for each rabbit on 1, 2, 3, 4 and 7 d.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Reversibility:
- not specified
- Remarks on result:
- not measured/tested
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 13.9
- Max. score:
- 80
- Reversibility:
- fully reversible
- Remarks on result:
- other: Individual scores were not cited.
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 10
- Reversibility:
- fully reversible
- Remarks on result:
- other: Individual scores were not cited.
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 14.4
- Max. score:
- 20
- Reversibility:
- fully reversible
- Remarks on result:
- other: Individual scores were not cited.
- Irritant / corrosive response data:
- The irritation index was calculated to be 13.9 for the cornea, 0 for the iris and 14.4 for the conjunctivae. The test substance caused moderate overall eye irritation to rabbits. Rinsing the eyes following instillation increased the effect of the test substance.
- Other effects:
- none
Any other information on results incl. tables
Results of the eye irritation:
Unwashed eyes after application of test substance:
Days after Appl. | Rabbit 1 (M) | Rabbit 2 (M) | Rabbit 3 (M) | ||||||
Cornea | Iris | Conjunctiva | Cornea | Iris | Conjunctiva | Cornea | Iris | Conjunctiva | |
1 | 15 | 0 | 18 | 10 | 0 | 18 | 10 | 0 | 16 |
2 | 15 | 0 | 16 | 10 | 0 | 18 | 10 | 0 | 14 |
3 | 15 | 0 | 16 | 10 | 0 | 18 | 10 | 0 | 14 |
4 | 15 | 0 | 16 | 10 | 0 | 18 | 10 | 0 | 10 |
7 | 10 | 0 | 10 | 10 | 0 | 14 | 0 | 0 | 6 |
Cornea: 10.6; Iris: 0; Conjunctivae: 14.8
Eyes washed after application of test substance:
Days after Appl. | Rabbit 4 (F) | Rabbit 5 (F) | Rabbit 6 (F) | ||||||
Cornea | Iris | Conjunctiva | Cornea | Iris | Conjunctiva | Cornea | Iris | Conjunctiva | |
1 | 10 | 0 | 18 | 20 | 0 | 18 | 15 | 0 | 20 |
2 | 10 | 0 | 16 | 40 | 0 | 18 | 15 | 0 | 20 |
3 | 10 | 0 | 14 | 40 | 0 | 16 | 15 | 0 | 18 |
4 | 5 | 0 | 10 | 40 | 0 | 14 | 15 | 0 | 18 |
7 | 0 | 0 | 0 | 20 | 0 | 14 | 5 | 0 | 6 |
Cornea: 17.3; Iris: 0; Conjunctivae: 14
R (effect of rinsing) = unrinsed eyes/rinsed eyes (i.e., 8.4/10.4) = 0.8
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritating to eyes) based on GHS criteria
- Conclusions:
- Under the conditions of the study, the test substance was considered to be moderately irritating to the eye.
- Executive summary:
An in vivo study was conducted to evaluate the acute eye irritation potential of the test substance (at ca. 82 % purity) in rabbits according to the procedure described in the Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics (1959) of the US Association of Food and Drug Officials (AFDO), which is similar to OECD Guideline 405 but with few deviations. 100 mg of the test substance was placed into the conjunctival sac of the left eye of six rabbits (i.e., 3 male and 3 females). The right eye remained untreated and was used as control. In three of the six rabbits, washing was done with 10 mL of lukewarm water after 30 seconds of test substance instillation. Assessment of ocular damage/irritation was made with a slit-lamp and scored for each individual rabbit approximately on 1, 2, 3, 4 and 7 d following treatment. The total maximum score was 80 for the cornea, 10 for the iris, 20 for the conjunctivae and 110 for the entire eye. The irritation index was calculated to be 13.9, 0 and 14.4 for the cornea, iris and conjunctivae, respectively. Rinsing the eyes following instillation increased the effect of the test substance. Under the conditions of the study, the test substance was considered to be moderately irritating to the eye.
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