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EC number: 600-026-8 | CAS number: 1000817-22-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solutions were prepared separately for each test concentration. The test solutions were prepared by adding test substance to 1L or 2L of test medium and stirring for approximately 1 hour. The test solutions appeared colorless and clear - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- Strain: SAG 61.81
- Source: Collection of algal cultures in University of Göttingen /Germany
- Age of inoculum (at test initiation): A stock algal culture is maintained continuously at the test facility and propagated on a weekly basis. Before the exposure an inoculum culture is prepared from the stock culture and incubated for 4 days at 24 °C (max. temperature difference 2 °C). After this time, the inoculum culture is in exponential growth phase and can be used to initiate the test (study day 0).
CULTURE CONDITIONS
- Inoculum:
- An inoculum culture in exponential growth phase was prepared with an aliquot of the stock algal culture added to sterile test media to provide an initial cell density of 10000 cells/ml.
- Incubation under test conditions for 4 days prior to test initiation
- Inoculum culture cell density (4 days growth) : 217E+4 cells/ml
- Inoculum culture morphology: normal and healthy
- Culturing media and conditions: The culture and test medium (USEPA medium or AAP, also according to ASTM) was prepared according to OECD Guideline for Testing of Chemicals, No. 201 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 25 °C
- pH:
- Study initiation: 7.6
Study end: 7.8 - 9.8 - Nominal and measured concentrations:
- Nominal test concentrations: 0, 0.56, 1.0, 1.8, 3.2, 5.6, 10 mg/L
Mean measured concentrations: -, 0.25b, 0.3b, 0.9b, 1.5, 3.0, 6.5 mg/L
b: measured concentration below limit of quantification - Details on test conditions:
- TEST SYSTEM
Inoculated replicates:
- Test vessels: Erlenmeyer flasks (nominal volume 250 mL) plugged with gas permeable silicone sponge caps
- Test volume: 100 mL
- Initial cell density: 1 x 104 cells/mL
- Replicates per test concentration: 3
- Replicates per test control: 3
*An additional replicate is prepared for 0h concentration control analysis only
Uninoculated replicate:
- Purpose: To correct for background fluorescence and for pH measurements. To determine the effect of the test system on test substance
stability.
- Test vessels: Erlenmeyer flasks (nominal volume 250 mL) plugged with gas permeable silicone sponge caps
- Test volume: 100 mL
- Replicates per test concentration/ control: 1
- Replicates per test control: 1
- Temperature measurement: Continuous measurement during the whole test period in the climate chamber in a flask with deionized water
- pH measurement: after 0 and 96 h
EFFECT PARAMETERS MEASURED:
- Algal growth measured as in vivo chlorophyll-a fluorescence (pulsed excitation with light flashes having a wavelength of 432 nm) after 96 h
- Determination of correlation between fluorescence and cell density:The fluorescence in the dilutions and the undiluted mixture were measured by single measurements and in parallel by direct microscopic cellcounting (two counts in a Neubauer haemocytometer).
- Algal morphology microscopically at the start of the test and at the end of the test (a pooled sample from each test concentration)
TEST CONCENTRATIONS
- Range finding study: yes
- Results used to determine the conditions for the definitive study (72 h): EC50 = 4.28 mg/L (fluorescence); ErC50 = 3.48 mg/L (nominal)
RECOVERY PHASE EVALUATION
- 0.5 mL aliquots of test solution from each replicate of the lowest concentration of test chemical which completely inhibited algal growth was used and combined these aliquots into a new test vessel
- Dilution 1:100 with fresh algal media to dilute the test substance to a concentration which does not affect growth (e.g. 1 mL inhibited algae + 99 mL fresh media)
- Incubation for a period of up to 9 days, and measurement of fluorescence every 2 days to check for algal growth. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.14 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.46 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.47 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 96 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 96 h
- Dose descriptor:
- EC90
- Effect conc.:
- 2.62 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 96 h
- Dose descriptor:
- EC90
- Effect conc.:
- 3.45 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Algal morphology: no remarkable observation at a concentration of 0, 0.56, 1.0, 1.8 and 3.2 mg/L (nominal); at a concentration of 5.6 and 10 mg/L (nominal) no algal cells were observed
- Based on results of the recovery phase investigation, the altered growth response between controls and test algae is due to algistatic effects. - Results with reference substance (positive control):
- ErC50 (72 h) = 1.36 mg/L
ErC50 (96 h) = 1.47 mg/L
Date of the last control experiment: 16 Feb 2009 - Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is toxic to freshwater algae.
Reference
Description of key information
EC50 (96h) = 2.14 mg/L and EC10 (96h) = 1.46 mg/L (measured) for Pseudokirchnerella subcapitata (OECD 201)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 2.14 mg/L
- EC10 or NOEC for freshwater algae:
- 1.46 mg/L
Additional information
The toxicity on aquatic algae was investigated for Fatty acids, C8-18 and C18-unsatd., reaction products with diethanolamine and propylene oxide (CAS 1000817-22-0) in a GLP guideline study according to the OECD 201 (BASF 2009). The analytical monitored study was conducted with the green algae species Pseudokirchneriella subcapitata. After 96 hours of exposure the ErC50 is determined to be 2.14 mg/L and the ErC10 is determined to be 1.46 mg/L based on measured concentrations.
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