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EC number: 251-257-2 | CAS number: 32846-21-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name : FAT 20003/K TE
Batch number : AT-0026132600
EAS Test item code : 2014-000180
Appearance / colour : solid / brown
Content of a.i. nominal : > 90 %w/w
Content of a.i. analysed : not available
Molecular weight : 517.60 g/mol (base) 540.59 g/mol (salt)
Expiry date: 20 Dec 2018
Storage conditions ambient (+5 to +30°C), dark, dry - Analytical monitoring:
- yes
- Details on sampling:
- After sampling, the test medium samples (0.5 mL) were mixed with 0.5 mL methanol and stored deep-frozen (below -18 °C) until analysis.
- Vehicle:
- no
- Details on test solutions:
- The necessary amount of FAT 20003/K TE for preparing the test solution was weighed on weighing scoops and transferred to a volumetric flask. Test medium was added up to the bench mark and the solution was homogenised by shaking. 50 mL of the prepared solution was transferred to each test vessel.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna STRAUS, Clone V, was used as the test organism. The animals are continuously bred in the laboratory and were originally purchased in a healthy condition from the Umweltbundesamt (Federal Environment Agency) in Berlin/Germany.
Daphnia magna was bred as group culture (5 to 20 daphnids per litre) in Elendt M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH. The animals were fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.
The daphnids were reared at a temperature of 20 ± 2 °C in a climatic chamber with 16 hours of illumination and 8 hours of darkness. The medium was changed three times per week. A pipette was used to separate the young daphnids from the adults.
Freshly hatched daphnids less than 24 hours old were used for the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 13 °dH (232 mg/L as CaCO3)
- Test temperature:
- 20.3 - 20.9 °C
- pH:
- 7.96 - 8.01
- Dissolved oxygen:
- >= 8.6 mg/L
- Nominal and measured concentrations:
- see Table below
- Details on test conditions:
- Temperature: 20.3 – 20.9 °C
Oxygen concentration: ≥ 8.6 mg/L
pH-value: 7.96 – 8.01
Exposure to light: 16 hours photoperiod /8 hours darkness daily
Feeding: none
Test vessels: four 100 mL glass beakers per concentration, each filled with 50 mL, one additional replicate for measurements without test organisms
Loading: 10 mL of test solution for each animal
Aeration: none
Number of animals: 20 per concentration in 4 replicates of 5 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 24 and 48 hours of exposure no immobilisation was observed in the control and at 100 mg/L.
- Results with reference substance (positive control):
- EC50 (24 h) between 1.0 and 2.0 mg/L
Reference substance: Potassium dichromate - Reported statistics and error estimates:
- The 24 h and 48 h EC50 are the estimated concentrations where 50 % of the daphnids were immobilised after 24 and 48 hours, respectively.
No statistical evaluation was indicated since no immobilisation occurred at the limit test concentration of 100 mg/L.
The NOEC was established based on the highest concentration at which the immobilisation is not higher than the allowed control immobilisation (>= 10 % immobilisation). - Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48 h) for immobilisation was determined to be >100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
- Executive summary:
The toxicity of FAT 20003/K TE on Daphnia magna was tested in a 48 hour acute immobilisation test. The test was performed according to OECD Guideline 202.
A static limit test with concentrations of 0 and 100 mg/L was performed. Test solutions were prepared by dilution of the test item in test medium and application of defined volumes of the test solutions to the test vessels. Endpoints reported are the EC50 and the NOEC after 24 and 48 hours. Temperature, pH-value and oxygen concentration of the test solutions measured after 0, 24 and 48 hours are reported. Hardness of the test water was measured on the day of application. Analytical samples taken at 0 hours (initial value) from fresh test solutions and after 48 hours from aged test solutions were analysed from control and 100 mg/L.
According to the results of the test, the EC50 (48 h) for immobilisation was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
Reference
The total hardness (as CaCO3) of the untreated control was determined to be 13°dH (232 mg/L CaCO3).
The mean pH-value of the untreated control was determined to be 7.96 +/- 0.01 (Std. Dev.).
The mean temperature of the control and all test item concentrations was measured to be 20.6 +/- 0.2 °C (Std. Dev.).
The mean oxygen concentration was determined to be 8.8 +/- 0.1 mg/L (Std. Dev.).
Description of key information
The toxicity of FAT 20003/K TE on Daphnia magna was tested in a 48 hour acute immobilisation test. The test was performed according to OECD Guideline 202. A static limit test with concentrations of 0 and 100 mg/L was performed. Test solutions were prepared by dilution of the test item in test medium and application of defined volumes of the test solutions to the test vessels. According to the results of the test, the EC50 (48 h) for immobilisation was determined to be > 100 mg/L (nominal). The corresponding NOEC (48 h) was 100 mg/L (nominal).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
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