Registration Dossier
Registration Dossier
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EC number: 250-333-2 | CAS number: 30772-79-3
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The ready biodegradability was investigated in a study conducted in accordance with a BOD Test for Insoluble Substances (BODIS), similar to ISO 10708, but not according to GLP. This is a ready biodegradability test method using unadapted domestic activated sludge at a concentration of 30 mg dw/L and a substrate concentration of 100 mg TOD/L. The test system is a two phase system with one third water and two-thirds air. The test can be regarded as well conducted and fulfils the validity criteria: The BOD/TOD ratio for the reference substance sodium benzoate reached the level of 60% within 14 days and was 76.7% after 28 days. The total oxygen uptake in the blanks was lower than 3 mg O2/L after the first week and was lower than 1 mg/L in the following weeks.
The test substance was added as a drop on a microscopic glass cover to enhance the bioavailability. The initial test concentration was 35.5 mg/L Vertral (TOD 2.825 mg O/mg test substance). The BOD/TOD for test substance steadily increased to 14% after 3 weeks and levelled off to 14.9% after 28 days. Under the conditions of this test system, Vertral is not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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