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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2017-09-07 to 2017-10-13
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: aerobic sludge of the mixed treatmant plant of urban (66%) and industrial (34%) liquid sewage
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sample of aerobic sludge of the mixed treatmant plant of urban (66%) and industrial (34%) liquid sewage situated at San Rocco - Monza (MB), Italia
- Storage conditions: aerobic
- Pretreatment: The mud samples have been analysed to check ity ability to form colony units, before their use. Than the mud sample has been centrifuged, washed and analyzed to quantify the suspended solids concentration for inoculum preparation
- Concentration of suspended solids: 4 mg/L
Duration of test (contact time):
28 d
Initial conc.:
19.66 mg/L
Based on:
TOC
Parameter followed for biodegradation estimation:
other: percentage ratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning
Details on study design:
TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 20°C
- Suspended solids concentration: 4mg/L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: glass bottles with hermetic seal
- Number of culture flasks/concentration: 17
- Method used to create aerobic conditions: stirring

SAMPLING
- Sampling frequency: TOC (total organic carbon) at test start, TIC (total iorganic carbon) weekly
- Sampling method: 3 replica per week per treatment

CONTROL AND BLANK SYSTEM
used treatments: Blank, test sample, Reference substance, Test sample + reference substance (not considered because the substance is resulted biodegradable)
Reference substance:
other: 1-Octanol
Parameter:
other: % degradation (ratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning)
Value:
1
Sampling time:
1 d
Key result
Parameter:
other: % degradation (ratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning)
Value:
62
Sampling time:
7 d
Parameter:
other: % degradation (ratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning)
Value:
82
Sampling time:
14 d
Parameter:
other: % degradation (ratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning)
Value:
97
Sampling time:
21 d
Parameter:
other: % degradation (ratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning)
Value:
88
Sampling time:
28 d
Results with reference substance:
The reference substance was degraded by 85% (degradation based onratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning) after 7 days.
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
7-Octen-1-al is considered readily biodegradable, because its level of biodegradability was 62% after 7 days (10 days window fulfilled) and reached 88% biodegradation within 28 days.
Executive summary:

The biodegradation of 7-Octen-1-al in water was determined in a GLP study according to OECD 310. % degradation was measured as ratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning. An initial concentration of 19.66 mg/L of OEL was inserted in the test vessels containing aerobic sludge of the mixed treatmant plant of urban (66%) and industrial (34%) liquid sewage. A degradation of 62% was determined within the 10 days window and reached a biodegradation of 88% after 28 days. The substance is therefore considered readily biodegradable.

Description of key information

Readily biodegradable: 88% after 28 days (62% within the 10 days window)

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The biodegradation of 7-Octen-1-al in water was determined in a GLP study according to OECD 310. % degradation was measured as ratio between the value of inorganic carbon measured and the value of organic carbon added at the beginning. An initial concentration of 19.66 mg/L of OEL was inserted in the test vessels containing aerobic sludge of the mixed treatmant plant of urban (66%) and industrial (34%) liquid sewage. A degradation of 62% was determined within the 10 days window and reached a biodegradation of 88% after 28 days. The substance is therefore considered readily biodegradable.