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EC number: 230-303-5 | CAS number: 7023-61-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Storage condition of test material: at room teperature at about 20 °C, away from direct sunlight
- Analytical monitoring:
- yes
- Details on sampling:
- The analytical measurements were based on the main component of the test item. Samples taken at the following timepoints:
- Just before the start of the test (duplicate samples from each test medium (without algae); duplicate samples from the control (without algae))
- After 72 hours (duplicate samples from each test medium (with algae) (stability samples); duplicate samples from the control (with algae)). For the 72-hour stability samples, additional flasks with algae were incubated for each test concentration separately under identical conditions as in the actual test. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Five WAFs (Water Accommodated Fractions) with loading rates of 3.2, 10, 32, 100 mg/L were prepared according to the OECD Guidance Document 23. The dispersions were prepared by mixing 8.0, 25.0, 80.0 and 250.2 mg of the test item into 2500 ml of test water, respectively, using ultrasonic treatment for 15 minutes. The dispersions were stirred at room temperature in the dark for 3 hours. After the stirring period of 3 hours, the saturated dispersions were filtered through a membrane filter. The filtrates of the dispersions with dissolved test item were tested on algae as WAFs. Due to technical reasons, the WAF with the lowest loading rate of 1.0 mg/L was prepared as a dilution of the WAF with the loading rate of 3.2 mg/L. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: 61.81 SAG
- Source: Supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, Göttingen / Germany). The algae are cultivated in RCC's laboratories under standardized conditions according to the test guidelines.
- Age of inoculum (at test initiation): 10000 algal cells/mL - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 24 mg/L as CaCO3
- Test temperature:
- 23 °C
- pH:
- 8.3 - 8.8
- Nominal and measured concentrations:
- - Nominal test concentrations: control, 1.0, 3.2, 10, 32, 100 mg/L as loading rates of the WAFs
- Mean measured concentrations: -, 0.026, 0.073, 0.053, 0.059, 0.064 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: Erlenmeyer flasks covered with glass dishes, 50 mL, 15 mL
- Initial cells density: 10000 algal cells /mL
- No. of vessels per concentration: 3
- No. of vessels per control: 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic test water
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH at the test start and the test end, temperature daily
OTHER TEST CONDITIONS
- Photoperiod: continuously light
- Light intensity and quality: 6800 - 7900 lux
EFFECT PARAMETERS MEASURED: cell density, shape and size of the algal cells microscopically
- Determination of cell concentrations: electronic particle counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2 (enlarged spacing factor was choosen since according to the results of the range-finding test a large concentration range had to be tested in the definitive test)
- Range finding study: yes - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 102 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: no effects observed up to the limit of water solubility
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Details on results:
- - Appearance of the test media: no remarkable observations, clear test medium
- Unusual cell shape: none
- Unusual cell size: none - Validity criteria fulfilled:
- yes
- Conclusions:
- With high probability acutely not toxic to aquatic algae.
Reference
Description of key information
With high probability acutely not toxic to aquatic algae.
Key value for chemical safety assessment
Additional information
The effect of the substance on the growth of Pseudokirchnerella subcapitata was investigated in a study following GLP and OECD guideline 201 (RCC Ltd, 2007). Due to the low solubility of the test substance a water accommodated fraction has been tested. The algae were exposed for 72 hours to the test substance. At test termination no effects were observed up to the limit of water solubility (at a nominal loading rate of 100 mg/L).
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