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EC number: 212-832-3 | CAS number: 872-85-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2 - 6 June 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP - guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Isonicotinaldehyde
- EC Number:
- 212-832-3
- EC Name:
- Isonicotinaldehyde
- Cas Number:
- 872-85-5
- Molecular formula:
- C6H5NO
- IUPAC Name:
- pyridine-4-carbaldehyde
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report) : Pyridine-4-aldehyde
- Physical state : clear yellowish liquid
- Analytical purity : 98,7% (GC)
- Lot/batch No. : 1120126/001
- Storage condition of test material : Refrigerator at approx. 4°C , in the dark , under nitrogen
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations : Samples of the test media were taken immediately before setting-in of the fish and after approximately 24 , 48 , 72 and 96 hours . At replacement of the test media at 72 hours samples of the "old" and the "freshly prepared" media were taken .
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method :
The test media were prepared by dissolving 28.5 , 60.0 , 104.4 , 181.6 and 341.8 (at the begin of the test) and 31.0 , 61.5 , 120.6 and 209.8 mg test substance (at 72 hours after begin of the test) in 6 litres dilution water each . No new medium was prepared for the highest concentration after 72 hours as no fish survived in this group .
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name : Zebrafish
- Source : Fa. Amazonas Aquarium , A-2840 Grimmenstein
- Age at study initiation (mean and range, SD) : no data
- Length at study initiation : approx 2,8 cm (mean of control group at the end of the study)
- Weight at study initiation (mean and range, SD): no data
- Feeding during test : none
ACCLIMATION
- Acclimation period : approx. 3 weeks
- Acclimation conditions : same as test
- Type and amount of food : TetraMin (Tetra Werke , Dr.rer.nat. Ulrich Baensch GmbH , D-49304 Melle)
- Feeding frequency : daily
- Health during acclimation (any mortality observed) : no data
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 250 mg CaCO3/l
- Test temperature:
- 20°C troughout the test period
- pH:
- 8,0 - 8,3 in the test substance groups
- Dissolved oxygen:
- at least 85% of the air saturation value at the appropriate temperature throughout the test period .
- Nominal and measured concentrations:
- see table 1 and 2 at "Any other information on materials and methods incl. tables"
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type : Test vessels were covered with transparent plastic lids
- Material : full glass vessels (29 x 20 x 24 cm) , containing 6 litre test medium
- Aeration : no aeration
- Renewal rate of test solution : As after 72 h the test substance concentration of the lowest concentration was slightly below 80% of the initial concentration the test media were replaced once after approx. 72 hours .
- No. of organisms per vessel : 7
- No. of vessels per concentration (replicates) : 1
- No. of vessels per control (replicates) : 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water : Local tap water was mixed with deionized water for achieving the appropriate water hardness (250 mg CaCO3/l)
- Intervals of water quality measurement :
* Total hardness of the control water
* pH in the control and test media at the start and after approx. 24,48,72 and 96 hours
* Dissolved oxygen concentration in the control and test media at the start and after approx. 24,48,72 and 96 hours
* The test substance concentrations in the test media were determined at the start and after 24,48,72 and 96 hours
* At replacement of the test media at 72 hours after start of the study the pH-value , temperature , dissolved oxygen concentration and the test substance concentrations of the "old" and the "freshly prepared" media were determined
OTHER TEST CONDITIONS
- Artificial light from 4 a.m. to 8 p.m.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Test concentrations : 5.6 , 10 , 18 , 32 and 56 mg/l
- Results used to determine the conditions for the definitive study : The concentrations in this test were chosen according to a preliminary test with 100 mg/l in which 3/3 fish died after 24 hours
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 42 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL : 42 - 42 mg/l
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL : 23 - 39 mg/l
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL : 14 - 24 mg/l
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 5.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities : In most fish that died decreased swimming activity and loss of equilibrium before death was noted
- Other biological observations : In the surviving animals there were no adverse effects noted
- Mortality of control : no - Results with reference substance (positive control):
- No reference substance testing reported
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mortality : Number of fish at the start of exposure : 7 in each group
Groups Concentration (mg/l) Total number of dead fish at hours (approx.) after start of exposure 0h 24h 48h 72h 96h Control 0 0 0 0 0 0 A 5,6 0 0 0 0 0 B 10,0 0 0 0 1 1 C 19,0 0 0 0 0 2 D 32,0 0 0 0 4 7 E 56,0 0 1 7 7 7 Except for the lowest concentration the test substance concentrations have been satisfactorily maintained over the test period .
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- pH and temperature were stable throughout the test period . Dissolved oxygen concentration was always higher than 80% of the air saturation at the appropriate temperature
- Conclusions:
- The study was conducted under GLP compliance and a well documented study report is available. The acute toxicity of pyridine-4-aldehyde to Zebrafish (Brachydanio rerio) was determined in a 96 hours semi-static test . The 96 h LC50 value was 18 mg/l (95% CL : 14 - 24 mg/l)
- Executive summary:
The acute toxicity to fish of pyridine-2-aldehyde was tested according to EU Method C.1 (Acute Toxicity for Fish) in a semi-static freshwater test with Zebrafish (Brachydanio rerio) as test organisms. The study was conducted under GLP compliance. Following a preliminary range-finding study, fish were exposed, in groups of seven, to an aqueous solution of the test material over a range of concentrations of 5.6, 10, 18, 32 and 56 mg/l for a period of 96 hours under semi-static test conditions. All animals were observed for alterations and behavioural changes at setting-in of the fish and about 2, 4, 24, 48, 72 and 96 hours afterwards.
The 96-Hour LC50 based on nominal test concentrations was 18 mg/l with 95% confidence limits of 14 - 24 mg/l.
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