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EC number: 950-480-9
CAS number: -
by activated sludge in presence of different concentrations of Algea
Fert Solid K+
Test item concentration
Oxygen concentration (mg/L)
n.d.: not determined as the values
dropped below the 2.5 mg O2/L value.
by activated sludge in presence of different concentrations of
Reference item concentration
for the test concentrations and for the controls and active sludge
respiration inhibition caused by test item after 3-h exposure
* negative values indicate that a
respiration greater than the mean respiration of two controls occurred,
as provided by biological variability
for the reference item concentrations and active sludge respiration
inhibition in comparison to controls after 3-h exposure
The toxicity of the test item Algea Fert Solid K+ to activated
sludge was assessed in a respiration inhibition study according to:
- EU Commission Directive EC 440/2008, C.11.
- OECD Guideline No. 209, “Activated Sludge, Respiration
Inhibition Test”, 1984.
For this purpose, aerobic activated sludge collected from
the recirculation tank of a municipal wastewater treatment plant was
incubated for 3-hours with different concentrations of the test item
under defined conditions.
The test item concentrations were 10.0, 17.8, 31.6, 56.2 and
100.0 mg/L. In addition to the five test item concentrations, two
untreated controls were incubated under the same conditions.
In order to check the normal sensitivity of the batch of
activate sludge, 3,5-dichlorophenol was used as a reference item. For
this purpose three nominal concentrations of 3,5-dichlorophenol were
tested, 5.0, 15.0 and 50.0 mg/L respectively.
The EC50of 3,5-dichlorophenol was found to be
11.79 mg/L, thus complying with the validity range indicated by the
referenced guidelines (EC50: 5-30 mg/L).
The respiratory rate expressed as oxygen consumption was measured
after three hours of exposure using a portable oximeter.
The inhibitory effect of the test item is expressed as a
percent of the mean respiratory rate of two controls. The results are
related to the nominal concentrations of the test item.
The respiration rate of the two controls was found to differ
by 10.8%, thus complying with the validity criteria indicated by the
mentioned guidelines (maximum difference between two replicates of
control = 15%).
Based on the results, the EC20, EC50and the
EC80for the test item were assessed to be greater than the
highest tested concentration, i.e. greater than 100 mg/L.
At the start of the test the pH of test water was 7.43. The pH
values and temperature of test solutions were measured in each test
flask during the oxygen determination: the pH was in the range 7.80 –
8.00, while the temperature was found to be in the range 19.7 – 20.3°C,
i.e. within the specified range (20.0± 2 °C).
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