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EC number: 950-480-9
CAS number: -
Lethal effect of
Algea Fert Solid K+ to zebra fish
test item concentration
n. of exposed
n. dead fish
The acute toxicity of the test item Algea Fert Solid K+ to
zebra fish (Danio rerio)was determined in a limit, 96‑hour static
test according to the following guideline:
- OECD Guideline for Testing of Chemicals, N. 203. “Fish, acute
toxicity test”, 1992,
- OPPTS 850.1075 guideline “Fish acute toxicity test, freshwater
and marine”, EPA Ecological effects Test guidelines, 1996.
For this purpose, juvenile fish of the speciesDanio reriowere
exposed, after an acclimation period, to an aqueous test medium
containing the test item at the only nominal concentration of 100.0
mg/L. Besides this test concentration, one tank without the test item
was prepared as the negative control.
The test organisms were checked for mortality after 2, 24, 48, 72
and 96 hours from the test initiation.
The pH values of the test medium after preparation was 7.29
for test item and 7.11 for control. Therefore, the pH of the test medium
was not adjusted before of the test starting.
Throughout the test, the dissolved oxygen was all the time above
60 per cent of the air saturation value both in the control and in the
test solution, as provided by OECD guideline.
The test temperature was between 22.3 andfor control tank and
between 22.3 andfor the test item solution tank, according to the OECD’s
recommended range (23.0±2.0°C).
Light intensity during the test period was daily measured and it
was in the range 118 – 126 for control tank and between 126 and 132 Lux
for test item solution tank, within the range 50-500 Lux.
In the control no mortality was observed during the test
period. This value complies with the validity criterion reported in the
guidelines (maximum mortality of one fish, if seven fish were used, in
the control medium at the end of the test).
No mortality was observed in the test item medium after 96
hours, therefore the NOEC (highest concentration tested without toxic
effects after the exposure period of 96 hours) of Algea Fert Solid K+ to
zebra fish was determined to be equal to the tested nominal
concentration of 100 mg/L (Fisher’s exact test).
Based on the observed effects, the LOEC and the 96-h LC50were
determined to be greater than the nominal tested concentration, 100.0
During the test period, any behaviour and/or pathological
signs on fishes exposed to the test item and in the control were
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