(3E)-1,7,7-trimethyl-3-(4-methylbenzylidene)bicyclo[2.2.1]heptan-2-one

Hydrolysis

Link to relevant study record(s)

Reference

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test: The hydrolysis of the test item was determined in lake water over 28 days using sterilised test vessels.
- Short description of test conditions: Test vessels were incubated in the dark at 25 ± 1 °C. Test solutions were shaken on a rotary shaker at 30 rpm. Flasks were covered with breathable membranes.
- Parameters analysed / observed: Test item concentration was determined by HPLC-DAD.

GLP compliance:

no

Remarks:

Published study from a peer-reviewed journal.

Radiolabelling:

no

Analytical monitoring:

yes

Details on sampling:

- Sampling intervals for the parent/transformation products: 0, 1, 2 6, 10, 15, 21, and 28 days
- Sampling method: 1 mL of water sample was withdrawn from each flask for the analysis.
- Sample storage conditions before analysis: Prepared samples were transferred to 2-mL amber glass vials and stored at -20 C until analysis.

Details on test conditions:

TEST SYSTEM
- Type, material and volume of test flasks, other equipment used: 250-mL Erlenmeyer flask
- Sterilisation method: Autoclave at 120 °C for 30 min
- Lighting: Constant darkness
- Measures taken to avoid photolytic effects: Flasks were wrapped with aluminium foil.
- Measures to exclude oxygen: None, flasks were covered with breathable membranes.
- Details of traps for volatile, if any: None
- If no traps were used, is the test system closed/open: Open

TEST MEDIUM
- Volume used/treatment: 100 mL
- Kind and purity of water: Lake water sample, filtered through a paper filter (pore size 3 to 5 μm).

OTHER TEST CONDITIONS
- Dissolved organic carbon: 5.66 mg/L

Duration:

28 d

pH:

7.6

Temp.:

25 °C

Initial conc. measured:

2 mg/L

Number of replicates:

3

Positive controls:

no

Negative controls:

no

Transformation products:

not measured

Key result

Remarks on result:

not determinable

Remarks:

No degradation observed

Details on results:

Degradation of the test item in sterilised lake water in the dark showed no significant (ANOVA, p <0.3) decrease in concentrations over 28 days, indicating that hydrolysis is of minor important for the removal of the test item in lake water.

Validity criteria fulfilled:

not specified

Conclusions:

There was no significant decrease in test item concentration in sterilised lake water in the dark over 28 days, indicating that hydrolysis is of minor importance for the removal of the test item in lake water.

Executive summary:

The hydrolysis of the test item was determined in a 28 day test. A nominal concentration of 2 mg/L test item was added to lake water and incubated in the dark at 25 ± 1 °C. Flasks were covered with breathable membranes and samples were taken at 0, 1, 2, 6, 10, 15, 21, and 28 days for analysis of the test item by HPLC-DAD. There was no significant decrease in test item concentration in sterilised lake water in the dark over 28 days, indicating that hydrolysis is of minor importance for the removal of the test item in lake water. This study is considered to be reliable with restrictions (Klimisch 2) as the test design was adequate, however there are limitations in the reporting of results.

Description of key information

No significant decrease in test item concentration by hydrolysis in water was detected over 28 days.

Additional information

A hydrolysis study was performed in which a nominal concentration of 2 mg/L test item was added to lake water and incubated in the dark at 25 ± 1°C for 28 days (2015). Flasks were covered with breathable membranes and samples were taken at 0, 1, 2, 6, 10, 15, 21, and 28 days for analysis of the test item by HPLC-DAD. There was no significant decrease in test item concentration in sterilised lake water in the dark over 28 days, indicating that hydrolysis is of minor importance for the removal of the test item in lake water. This study is considered to be reliable with restrictions (Klimisch 2) as the test design was adequate, however there are limitations in the reporting of results.