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EC number: 244-168-5
CAS number: 21041-95-2
The results of the optical density (OD) measured at 570 nm of each
sample and the calculated relative viability % values are presented below:
Optical Density mean (OD) and the calculated relative viability % of the
Optical Density (OD)
Phosphate buffered saline
5% (w/v) SDS solution
1. Mean blank value was 0.047.
2. Optical density means the mean value of the duplicate wells for each
An in vitro skin corrosivity
and irritation test
of cadmium hydroxide was
performed in a reconstructed human epidermis model.EPISKINTM(SM)
is designed to
predict and classify thecorrosivity and irritation potential
of chemicals by measuring its cytotoxic effect as reflected in the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
The corrosivity and irritation potential of the test item was evaluated
according to theOECD No. 431 and No. 439 guidelines.
Disks of EPISKINTM(SM)
with the test
incubated for 15 minutes (irritation testing)
and 4 hours (corrosivity testing) at
room temperature. Exposure of the test item was terminated by rinsing
with Phosphate Buffered Saline (PBS). The epidermis
units were then incubated at 37°C for 42 hours in an incubator with 5% CO2,in
a > 95% humidified atmosphere (irritation
viability of each disk was assessed by incubating the tissues for
3 hours with MTT solution at 37°C in an incubator with 5% CO2
protected from light, in a > 95% humidified atmosphere.
The precipitated formazan crystals were then extracted using acidified
isopropanol and quantified spectrophotometrically.
Physiological saline (0.9% (w/v) NaCl solution) treated epidermis
were used as negative control and glacial acetic acid treated epidermis
were used as positive control (two units/control) in case of the
corrosivity testing. PBS treated epidermis were used as negative control
and 5 % (w/v) Sodium Dodecyl Sulphate (SDS) solution treated epidermis
were used as positive control (three units/control) in case of the
irritation testing. Two additional disks were used to provide in each
case an estimate of colour contribution (NSCliving) from the
test item. For each treated tissue, the viability was expressed as a %
relative to the negative control. For corrosivity, if the mean relative
viability is <35% the test item is considered to be corrosive to skin.
For irritation, if the mean relative viability after 15 minutes of
exposure and 42 hours post incubation is less or equal (≤) to 50% of the
negative control, the test item is considered to be irritant to skin.
Following exposure with cadmium hydroxide, the mean cell viability
was 88.2% compared to the negative control. This is above the threshold
of 35%, therefore the test item was considered as being non-corrosive
Following exposure with cadmium hydroxide, the mean
cell viability was 93.1% compared to the negative control. This is above
the threshold of 50%, thereforethe test item was considered
as being non-irritant to skin.
The experiment met thevalidity criteria, therefore the study was
considered to be valid.
In conclusion, in this in vitro EPISKINTM(SM)
model test with Cadmium
hydroxide, the results indicate that
the test item is not corrosive and not irritant to the skin, UN GHS
Classification: No Category.
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