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EC number: 219-006-1 | CAS number: 2312-35-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22nd April 2001 to 8 September 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Duplicate aliquots (approximately 530 mL) were removed from each test vessel at 24 hours prior to the addition of the Daphnia, at 0 hours and at 48 hours.
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 50.16 mg test material was dissolved in 50 mL acetone and serially diluted in acetone to prepare stock solutions
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): 6-24 hours old
- Method of breeding: acyclical parthenogenesis
- Feeding during test: none - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20.9-21.3 ºC
- pH:
- 7.56-7.94
- Dissolved oxygen:
- ≥80 % of air saturation value
- Nominal and measured concentrations:
- 0, 0.00625, 0.0125, 0.05 and 0.1 ppm (nominal)
0, 0.004, 0.013, 0.015, 0.051 and 0.08 ppm (measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass moulded beakers of approximately 3 litre capacity containing Daphnia test chambers
- Material, size, headspace, fill volume: test chambers were constructed of 250 mesh stainless steel (38 mm depth, 75 mm diameter); fill volume of test vessels - approximately 2 litres of solution
- Type of flow-through: test medium from a header tank was passed into a glass mixing vessel at each test concentration; medium was mixed with a continuous flow of stock solution of test material; stock solutions were delivered using Medfusion Syringe Infusion Pumps; mixing vessels were placed on magnetic stirrers to ensure adequate mixing of test solutions and each mixing vessel contained one outlet leading directly to the appropriate test and control vessels.
- Renewal rate of test solution: flow rate - 14 mL/min
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Conductivity: 477-562 µS
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light; 8 hours dark
- Light: illumination was provided by artificial daylight fluorescent tubes
RANGE-FINDING STUDY
- Test concentrations: 0, 0.001, 0.01, 0.1 and 1 ppm
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.014 other: ppm
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % C.L. 0.03-0.046
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.014 other: ppm
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % C.L. 0.011-0.019
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.015 other: ppm
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.004 other: ppm
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - After 24 h exposure, 100 % immobile Daphnia were observed at 0.08 ppm, 65 % at 0.051 ppm and 5 % at 0.013 ppm. By 48 hours, 95 % immobile Daphnia were recorded at 0.051 ppm, 45 % at 0.015 ppm , 35 % at 0.013 ppm and 15 % at 0.004 ppm.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the test, the 48 hour EC50 to Daphnia magna in a flow-through test system, based on mean measured concentrations, was 0.014 ppm. The 48 hour NOEC was 0.004 ppm.
- Executive summary:
The EC50 for immobilsation of Daphnia exposed to propargite was determined over a 48 hour period in a study conducted in line with standardised guidelines OECD 202 and EU Method C.2.
The nominal concentrations of propargite tested were 0, 0.00625, 0.0125, 0.025, 0.05 and 0.1 ppm with the corresponding overall mean measured concentrations being 0.000, 0.004, 0.013, 0.015, 0.051 and 0.080 ppm, respectively. Chemical analysis of samples taken at 0 and 48 hours indicate that the concentrations were reasonably well maintained over the test period. Propargite was poorly soluble and was added to the test system with acetone as a co-solvent. No undissolved test material was observed in the test vessels.
Four vessels were prepared at each test concentration with a solvent and non-solvent control included. Five Daphnia were added to each test vessel.
The EC50 and NOEC were estimated based in an overall mean measured concentrations of propargite:
95 % confidence limits Timepoint (h) EC50 value (ppm) Lower limit Upper limit NOEC (ppm) 24 0.038 0.030 0.046 0.015 48 0.014 0.011 0.019 0.004 Test solution quality parameters were measured at the beginning and at 24 hour intervals at all concentrations. All parameters remained within acceptable limits.
Reference
Range-finding test
After 24 hours exposure, all Daphnia at 1 ppm were immobile with 50 % immobile at 0.1 ppm. After 48 hours exposure, all Daphnia at 0.1 ppm were immobile with 10 % immobile at 0.01 ppm. No other immobile Daphnia were recorded at any other test concentration.
Definitive test
Table 1: Cumulative number of immobile Daphnia
Concentration (ppm) | ||||||||
Time (h) | Replicate | Non-solvent control | Solvent control | 0.004 | 0.013 | 0.015 | 0.051 | 0.08 |
24 | A | 0 | 0 | 0 | 0 | 0 | 3 | 5 |
B | 0 | 0 | 0 | 0 | 0 | 5 | 5 | |
C | 0 | 0 | 0 | 1 | 0 | 2 | 5 | |
D | 0 | 0 | 0 | 0 | 0 | 3 | 5 | |
% immobile | 0 | 0 | 0 | 5 | 0 | 65 | 100 | |
48 | A | 0 | 0 | 0 | 1 | 3 | 5 | 5 |
B | 0 | 0 | 1 | 2 | 4 | 5 | 5 | |
C | 0 | 0 | 0 | 3 | 1 | 5 | 5 | |
D | 0 | 0 | 2 | 1 | 1 | 4 | 5 | |
% immobile | 0 | 0 | 15 | 35 | 45 | 95 | 100 |
Description of key information
48 hour LC50 0.014 mg/L; study conducted in accordance with OECD Guideline 202 and EU Method C.2; Knight and Allan, 2002
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.014 mg/L
Additional information
The nominal concentrations of propargite tested were 0, 0.00625, 0.0125, 0.025, 0.05 and 0.1 ppm with the corresponding overall mean measured concentrations being 0.000, 0.004, 0.013, 0.015, 0.051 and 0.080 ppm, respectively. Four vessels were prepared at each test concentration with a solvent and non-solvent control included; five Daphnia were added to each test vessel.
The EC50 and NOEC were estimated based in an overall mean measured concentrations of propargite:
95 % confidence limits | ||||
Timepoint (h) | EC50 value (ppm) | Lower limit | Upper limit | NOEC (ppm) |
24 | 0.038 | 0.030 | 0.046 | 0.015 |
48 | 0.014 | 0.011 | 0.019 | 0.004 |
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