Registration Dossier
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EC number: - | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 (72h) > 100 mg/l
Key value for chemical safety assessment
Additional information
The acute toxicity to Algae of the test item was assessed by its single components.
The influence of C1 on the growth of the green algal species Pseudokirchneriella subcapitata, formerly known as Selenastrum capricornutum, was investigated in a 72-hour limit test according to the OECD Guideline No. 201. On this purpose, exponentially growing test algae were exposed for 72 hours to an aqueous test medium solution at the only nominal concentration of 100.0 mg/L, under defined conditions. Besides this concentration, a negative control without the test item was also prepared to check the acceptability of the algal inoculum.Algal cell density was measured every 24 hours by fluorescent reading with a spectrofluorophotometer in few millilitre samples taken from each test concentration replicate and from controls. In the test item solution, at the nominal concentration of 100.0 mg/L, no significant biological effects were shown in comparison with negative control for both the endpoints, yield and growth rate, after 72 hours of exposure (Bonferroni adjusted test). Therefore, the 72-h EyC10,EyC20, EyC50, and 72-h ErC10,ErC20,ErC50, were determined to be higher than the nominal tested concentration (100 mg/L).
The influence of C2 on the growth of the green algal species Pseudokirchneriella subcapitata, formerly known as Selenastrum capricornutum, was investigated in a 72-hour full test according to the OECD Guideline No. 201, 2006. For this purpose, exponentially growing test algae were exposed for 72 hours to an aqueous test medium containing the test item at five nominal concentrations in a geometric series, namely 10.0, 17.8, 31.6, 56.2 and 100.0 mg/L, under defined conditions. Algal cell density was measured every 24 hours by fluorescent reading with a spectrofluorophotometer in few millilitre samples taken from each test concentration replicate and from controls. In the test item solution the algal growth rate (r) after 72 hours of exposure was inhibited by a minimum value of 1.0% for the nominal concentration of 17.8 mg/L to a maximum value of 39.7% for the highest concentration tested, compared to the negative control.The EC50 based on growth rate was therefore found to be > 100 mg/l nominal concentration.
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