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EC number: 876-151-9 | CAS number: 2292123-68-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Version / remarks:
- July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Testing Methods for New Chemical Substances (301C) - Japan CSCL
- Version / remarks:
- March 29, 2018
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): 10 locations in Japan (samples from: surface water, surface soil of rivers, lake, and inland sea, return sludge from sewage plants)
The activated sludge was cultivated for 18.5 hours after feed with synthetic sewage. Synthetic sewage was prepared as follows: glucose, peptone and potassium dihydrogenphosphate were dissolved in purified water, pH adjusted to 7.0 +/- 1.0 - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Remarks:
- The test substance did not dissolve.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The basal culture medium (5L) was prepared at the same proportion as the following method: each 3µ aliquots of A, B, C, and D described in JIS K 0102:2016 Section 21, was added to purified water. Solution was made up to 1L. pH adjusted to 7.0.
- Solubilising agent (type and concentration if used): Not applicable
- Test temperature: 24.4 - 23.5°C
- pH: 7.0
- pH adjusted: no
- Continuous darkness: yes
- suspended solids concentration: 30 mg/L
TEST SYSTEM
- Number of culture flasks/concentration: 3 flasks
- Method used to create aerobic conditions: Each test solution was stirred with a stirrer
- Measuring equipment: Closed system oxygen consumption measuring apparatus (No. CM-055)
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: not applicable
SAMPLING
- Sampling frequency: continuous
- Sterility check if applicable: yes
- Sample storage before analysis: not applicable
- Other: At end of study duration, pH and HPLC measurement were taken in the control, (test item + water) and toxicity control (test item + sludge)
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
STATISTICAL METHODS:
Values were rounded off in accordance with JIS Z 8401:1999 rule B. - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Parameter:
- % degradation (test mat. analysis)
- Remarks:
- Measured using HPLC
- Value:
- 1
- Sampling time:
- 28 d
- Results with reference substance:
- Aniline - Day 14 - 92% Degradation.
Validation criteria were met. - Validity criteria fulfilled:
- yes
- Remarks:
- see Table 4 in results section
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance is not readily biodegradable under the conditions of the study.
Reference
Table 1: Appearances and pH of the test solutions were as follows:
Test solution | Appearance | pH | |
At Start of incubation | Water + test item | The test item was not dissolved. The test solution was colorless | |
Sludge + test item | The test item was not dissolved. The test solution was colorless | -
| |
Control blank | Sludge observed. Test solution was colorless | - | |
At end of incubation | Water + test item | Insoluble compound was observed. The test solution was colorless | Vessel No. 1: 7.2 |
Sludge + test item | Insoluble compound in addition to sludge was observed. Growth of the sludge was not observed. The test solution was colorless | Vessel No. 2: 7.4 Vessel No. 3: 7.4 Vessel No. 4: 7.4 | |
Control blank | Sludge observed. Test solution was colorless | Vessel No. 5: 7.5 |
Table 2: Analytical results of test solutions
Water + test item | Sludge + test item | Theoretical amount | ||||
Vessel No.1 | Vessel No. 2 | Vessel No. 3 | Vessel No. 4 | |||
BOD1 | mg | 0.5 | 1.1 | 0.2 | -0.8 | 58.2 |
Residual amount and percentage of test item (by HPLC) | mg | 29.9 | 30.2 | 29.7 | 29.3 | 30.0 |
% | 100 | 101 | 99 | 98 | - |
1The value of the test solution (control blank) was subtracted from the values of the test solutions (sludge + test item)
Table 3: % biodegradation on Day 28
Sludge + test item | |||||
Vessel No. 2 | Vessel No. 3 | Vessel No.4 | Average | ||
BOD | % | 2 | 0 | -1 | 0 |
Test item (by HPLC) | % | -1 | 1 | 2 | 1 |
Table 4: Validity of the test conditions
Values in study | Criterion value | ||
Difference between extremes of replicate values of % biodegradation in test olutions (sludge + test item) on Day 28
| BOD | 3% | <20% |
Test item (by HPLC) | 3% | ||
% biodegradation by BOD in test solution (sludge + aniline) | Day 7 | 75% | >40% |
Day 14 | 92% | >65% | |
BOD in test solution (control blank) | Day 28 | 35 mg/L* | <60 mg/L |
*Measurement value (10.6mg/L)/[test volume (300mL) /1000] = 35 mg/L
Description of key information
In a study conducted in accordance with OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I), 0% biodegradation was recorded after 28days (O2 consumption).The test substance is not readily biodegradable under the conditions of the study.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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