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EC number: 619-020-1 | CAS number: 94361-06-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 28 Jul 2006 to 29 Aug 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 221 (Lemna sp. Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.4400 (Aquatic Plant Toxicity Test using Lemna spp. Tiers I & II))
- Version / remarks:
- 1996
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Lemna gibba
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Reference substance (positive control):
- no
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 200 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- dry weight
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 59 µg/L
- 95% CI:
- >= 51 - <= 67
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- frond number
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 67 µg/L
- 95% CI:
- >= 58 - <= 76
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- dry weight
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- 97 µg/L
- 95% CI:
- >= 46 - <= 150
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Validity criteria fulfilled:
- yes
- Remarks:
- See validity criteria in 'Any other information on results incl. tables'
- Conclusions:
- In a aquatic plant growth inhibition test performed following OECD TG 221 and OPPTS 850.4400 guidelines the 7-day NOErC and ErC50 for Lemna gibba were determined to be 25 and > 200 µg/L, respectively.
- Executive summary:
Growth inhibition caused by the test substance to the freshwater plant Lemna gibba was determined in a 7-day semi-static test according to OECD TG 221 and OPPTS 850.4400 guidelines. The study was compliant with GLP principles. Lemna plants were exposed to nominal test substance concentrations of 0 (control), 6.25, 12.5, 25, 50, 100 and 200 µg/L. The concentrations of the test item in freshly prepared test solutions were found to be in the range 88 -120 % of the nominal values and in the old test solutions at renewals were in the range 79 - 103 % of the nominal values. Nominal concentrations were used for the calculation and reporting of results. Test vessels were maintained under continuous illumination of 8530 - 9050 lux. Temperature was 23.1 to 25.4 ºC and pH ranged between 7.60 and 8.30.
The initial number of fronds in all test concentrations and the control was 11. After 7 days of exposure the mean frond number in the control was 109. The initial weight of 11 fronds on Day 0 was 1.1 mg. After 7 days of exposure the mean frond dry weight in the control was 15.9 mg. The results of Dunnett's test showed that for increase in frond number and growth rate the three highest test item treatments are significantly different to the control. For area under the growth curve and increase in dry weight the four highest test item treatments are significantly different to the control.Therefore, the 7-day NOErC was determined to be 25 µg/L based on nominal concentrations. The 7-day ErC50 was determined to be > 200 µg/L based on nominal concentrations.
Reference
The concentrations of the test item in freshly prepared test solutions were found to be in the range 88 -120 % of the nominal values and in the old test solutions at renewals were in the range 79 - 103 % of the nominal values, see Table 1. Nominal concentrations were used for the calculation and reporting of results.
The initial number of fronds in all test concentrations and the control was 11. After 7 days of exposure the mean frond number in the control was 109. The initial weight of 11 fronds on Day 0 was 1.1 mg. After 7 days of exposure the mean frond dry weight in the control was 15.9 mg. The results of Dunnett's test showed that for increase in frond number and growth rate the three highest test item treatments are significantly different to the control. For area under the growth curve and increase in dry weight the four highest test item treatments are significantly different to the control. Results on effect concentration are shown in Table 2.
Visual observations at Days 3, 5 and 7 (which were not analyzed statistically), showed more than 30% of the test population exhibited visual symptoms of toxicity at the three highest concentrations (nominally 50, 100 and 200 μg/L). On Days 5 and 7 fronds appeared stunted, and some new fronds were also browned (on Day 7 only). Additionally, at Day 3, between 11 and 30% of the test population exhibited the same symptoms of toxicity at 100 μg/L, and at Day 7 the same symptoms were seen at 25 μg/L.
Table 1, Analytical Results
Nominal conc. (μg/L) | Measured % of nominal in fresh solutions | Measured % of nominal in old solutions | ||
0-d | 4-d | 4-d | 7-d | |
0 (control) | <LOQ | <LOQ | <LOQ | <LOQ |
6.25 | 88 | 98 | 87 | 97 |
12.5 | 102 | 93 | 82 | 79 |
25 | 120 | 95 | 91 | 81 |
50 | 100 | 93 | 83 | 89 |
100 | 111 | 89 | 87 | 83 |
200 | 113 | 97 | 103 | 90 |
<LOQ – less than the limit of quantification (0.05 μg/L)
Table 2, Effect of the test substance on Lemna gibba
| Cyproconazole μg/L | |||
| EC50 value | NOEC | LOEC | |
| 7-day value | 95% confidence interval |
|
|
Increased frond number | 59 | 51-67 | 25 | 50 |
Growth rate | >200 | - | 25 | 50 |
Area under the growth curve | 97 | 46-150 | 12.5 | 25 |
Increase in dry weight | 67 | 58-76 | 12.5 | 25 |
Validity criteria
The validity criteria were met, with the frond numbers in the controls more than doubling within 2.5 days, resulting in a greater than 7-fold increase in frond number. The increase in frond number in all control replicates at 7 days was between 9- and 10-fold.
Description of key information
One study on a freshwater higher plant was performed and included here as a supporting study.
Freshwater, 7-d NOErC = 25 µg/L (nominal concentrations), Lemna gibba, OECD TG 221, OPPTS 850.4400, Everett et al 2007
Freshwater, 7-d ErC50 = > 200 µg/L (nominal concentrations), Lemna gibba, OECD TG 221, OPPTS.4400, Everett et al 2007
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 200 µg/L
- EC10 or NOEC for freshwater plants:
- 25 µg/L
Additional information
There is one standard guideline followed and GLP compliant study available for this endpoint, which is included here as supporting study for freshwater conditions. The supporting study was performed with the duckweed species Lemna gibba. Plants were exposed to nominal test substance concentrations of 0 (control), 6.25, 12.5, 25, 50, 100 and 200 µg/L. The concentrations of the test item in freshly prepared test solutions were found to be in the range 88 -120 % of the nominal values and in the old test solutions at renewals were in the range 79 - 103 % of the nominal values. Based on nominal concentrations, the 7-day NOErC and the 7-day ErC50 were determined to be 25 µg/L and > 200 µg/L, respectively (Everett et al 2007, OECD TG 221, Reliability 1).
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