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Diss Factsheets
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EC number: 259-452-4 | CAS number: 55039-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Remarks:
- Evaluation of inherent biodegration and COD/BOD5
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Justification for read across is detailed in the report attached to the IUCLID section 13.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- yes
- Remarks:
- The test substance was running in duplicate instead in triplicate.
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes
- Specific details on test material used for the study:
- None
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Mixture of polyvalent bacteria collected on February 13, 1996 in the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal
- Details on inoculum:
- Test organismStrain / Inoculation Mixture of polyvalent bacteria collected on February 13, 1996 in the aeration tank of a domestic sewage treatment plant, PRO RHENO Basel communal.Bacteria concentration : 100 mg/l suspended solids
- Duration of test (contact time):
- 28 d
- Details on study design:
- This test method was based on the following conditions:- test chemicals as sole organic carbon sources- no adaptation of micro-organisms to the test chemicalsTested chemicals were inoculated in the testing vessels with micro-organisms. During the test period, the Biochemical Oxygen Demand was measured continuousily by means of a BOD-meter. Biodegradability was calculated on the basis of the theoretical oxygen demand (ThOD) or less satisfactorily COD, if the ThOD could not be calculated.Optionally, primary biodégradation could be determinated by analysis of dissolved organic carbon (DOC) at the beginning and the end of the test.
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- [Merck No. 6290]
- Preliminary study:
- None
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: Standard deviation is not available
- Details on results:
- None
- Results with reference substance:
- Biodegradation after 28 days of the reference substance was 88 %
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Biodegradation of the test substance after 28 days was 0 %.
- Executive summary:
A key study was performed to determine the percent biodegradation. An inherent biodegradability te Similar subsyance was determined in a 28 days MITI (II) - Test according to the OECD Guideline for Testing of Chemicals, No. 302C. The test substance was tested in a concentration of 30 mg/l. The reference substance (Sodium benzoate) was tested in a concentration of 100 mg/l. Based on the results of the study, biodegradation of the test substance after 28 days was 0 %.
Reference
None
Description of key information
Not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
BIOWIN estimates the probability of rapid aerobic and anaerobic biodegradation of an organic compound in the presence of mixed populations of environmental microorganisms. BIOWIN contains seven separate models. According to Ready Biodegradability Prediction outcome, the substance is considered no readily biodegradable.
Similar substance 01 and was evaluated for biodegradability.
A key study was performed to determine the percent biodegradation of Similar substance 01. An inherent biodegradability was determined in a 28 days MITI (II) - Test according to the OECD Guideline for Testing of Chemicals, No. 302C. The test substance was tested in a concentration of 30 mg/L. The reference substance (Sodium benzoate) was tested in a concentration of 100 mg/L. Based on the results of the study, biodegradation of the test substance after 28 days was 0 %.
In another key study, chemical oxygen demand COD was calculated according to DIN 38409 - H 41-1 (1980) corresponding to EEC Directive 92/69/C.6 (1992). The test substance 01 was tested with a sample weight of 3.53 and 3.76 mg. The reference substance was Potassium hydrogen phthalate. It was tested with 20 mL of a 0.17 g/L solution. The COD of the Similar substance 01 was found to be 824 mg 02/g.
A supporting study was performed to determine the biochemical oxygen demand of the Similar substance 01, by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C. Based on the findings from the study, the BOD5 was found to be 19.0 mg O2/g test mat.
Several data on BOD5 and COD are also available on Similar substance 02.
The data Biochemical Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) are the most commonly used parameters for the characterization of the biodegradability capacity. COD results are typically higher than BOD5 values, and the ratio vary depending on the characteristics of the substrate. This ratio has been commonly used as an indicator for biodegradation capacity, the "Biodegradability index" (B.I.).
As indicated in ECHA Guidance Requirement R.7B, the BOD5 test is a traditional biodegradation test that is now replaced by the ready biodegradability tests. Therefore, this test should not be performed today for assessment of the ready biodegradability of substances. Older test data may, however, be used when no other degradability data are available. If the ratio BOD5/COD is => 0.5, the substance is regarded as being readily biodegradable.
The ratio BOD5/COD of the found BOD5 and COD values is less than 0.5 (if => 0.5, the substance is regarded as being readily biodegradable), therefore the substances showed no ready biodegradability.
Additionally, by the nature of their design and use, acid dyes are not intended to be readily biodegradable as this would assist in the rapid destruction of the dyestuff, rendering it unfit for purpose. As such, it is accepted that such substances are not readily biodegradable under relevant environmental conditions. A published study (Pagga & Brown, 1986) describes the results of the testing of 87 dyestuffs in short-term aerobic biodegradation tests. The authors of this publication concluded that dyestuffs are very unlikely to show any significant biodegradation in such tests and that 'there seems little point in carrying out such test procedures’ on dyestuffs. There are ISO, European, American (AATCC) and national standards for the color fastness of dyes. Dyes are required to have specific fastness properties. If the dyes were biodegradable, it would not be possible for them to have these fastness properties.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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