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EC number: 240-185-7 | CAS number: 16044-24-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 04-04-1984
- Principles of method if other than guideline:
- SOP 4.006
The guideline was named "Daphnia sp., Acute Immobilisation Test and Reproduction Test" Part II - the reproduction test at the time. - GLP compliance:
- not specified
Test material
- Reference substance name:
- Methanaminium, N-[4-[[4-(dimethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, ethanedioate
- EC Number:
- 241-922-5
- EC Name:
- Methanaminium, N-[4-[[4-(dimethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, ethanedioate
- Cas Number:
- 18015-76-4
- Molecular formula:
- C25H26N2O4
- IUPAC Name:
- Methanaminium, N-[4-[[4-(dimethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]-N-methyl-, ethanedioate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Basic Green 4 Oxalate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution of nominal 100 mg/L was diluted to the final concentration. Dilution water is filtrated surface water of a gravel pit near Leverkusen.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Federal Health Agency, Berlin, Germany
- Feeding during test
- Food type: green algae (D. subspicatus)
- Amount: 0.1 - 0.4 mL solution
- Frequency: daily
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: sieving
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 19 - 21°C
- pH:
- 8.0 - 8.4
- Dissolved oxygen:
- 7.7 - 9.2 mg/L
- Nominal and measured concentrations:
- Nominal: 0 (control), 0.0081, 0.026, 0.081, 0.26, 0.81 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, h = 11.5 cm, diameter: 9.5 cm, 250 mL test medium
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 8
- No. of vessels per control (replicates): 8
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 light / 8 dark
- Light intensity: ca. 6000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 3.1
- Range finding study
- Results used to determine the conditions for the definitive study: 48-h EC50 = 0.81 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.008 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks:
- offspring/maternal animal
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks:
- maternal
- Details on results:
- No mortality occurred in the negative control. However, the reproduction rate of 40 offsprings / maternal animal was not reached, so the test is not considered completely valid.
Any other information on results incl. tables
Reproduction rates (x offspring / maternal animal):
Control = 11.03 (considered as 100%)
0.0081 mg/L = 5.4 (49%)
0.026 mg/L = 6.4 (58%)
No offspring at 0.081, 0.26 and 0.81 mg/L due to 80 -100% maternal mortality.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Remarks:
- The reproduction rate in the negative control was too low.
- Conclusions:
- The 21-d NOEC (reproduction) to Daphnia magna was found to be <0.0081 mg/L. The reproduction toxicity might be overestimated due to the low reproduction rate in the whole test (negative control, too). However, due to the low 48-h EC50 (mortality) in the range finding study and the high maternal mortality rate in the main test, the substance can be considered chronically very toxic to aquatic invertebrates.
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