Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 238-101-9 | CAS number: 14233-37-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 MAY 2019 to 14 JUNE 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Commission Regulation (EC) No. 440/2008, Method C.2. “Daphnia sp. Acute Immobilisation Test”, adopted 30. May 2008
- Deviations:
- yes
- Remarks:
- The temperature in the experiment was in the range 19.7 – 23.0 °C and therefore fluctuates by more than ± 1°C. As no immobility was observed in the blank control this was stated as uncritical
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD Guideline for Testing of Chemicals No. 202, adopted 13. Apr. 2004:
”Daphnia sp., Acute Immobilisation Test“ - Deviations:
- yes
- Remarks:
- The temperature in the experiment was in the range 19.7 – 23.0 °C and therefore fluctuates by more than ± 1°C. As no immobility was observed in the blank control this was stated as uncritical
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,4-bis(isopropylamino)anthraquinone
- EC Number:
- 238-101-9
- EC Name:
- 1,4-bis(isopropylamino)anthraquinone
- Cas Number:
- 14233-37-5
- Molecular formula:
- C20H22N2O2
- IUPAC Name:
- 1,4-bis[(propan-2-yl)amino]-9,10-dihydroanthracene-9,10-dione
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1 µg/L
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dissolution of test item.
- Eluate:
- Differential loading:
- Controls:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no
- Other relevant information:
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Berlin
- Justification for species other than prescribed by test guideline:
- Age at study initiation (mean and range, SD): between 0 and 24 hours
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD):
- Stage and instar at study initiation:
- Valve height at study initiation, for shell deposition study (mean and range, SD):
- Peripheral shell growth removed prior to test initiation:
- Method of breeding: In-house breeding since 27. September 2007
- Source: Umweltbundesamt Berlin
- Age of parental stock (mean and range, SD):
- Feeding during test: green algae (Desmodesmus subspicatus)
- Food type: green algae (Desmodesmus subspicatus)
- Amount:
- Frequency:
ACCLIMATION
- Acclimation period: 0.5 hours
- Acclimation conditions (same as test or not): yes
- Type and amount of food: green algae (Desmodesmus subspicatus)
- Feeding frequency:
- Health during acclimation (any mortality observed): no
QUARANTINE (wild caught)
- Duration:
- Health/mortality:
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS:
18 hours and 30 minutes before the start of the test, the adult animals were separated
from the young. 0.5 hours before test start, the adults were caught with the help of a glass
tube, and the newborn daphnia (age < 24h) were sieved from the medium and immediately placed into a beaker containing dilution water. After the settling-in period, animals which
showed no apparent damage were used for the test.
Switching from M4-medium (husbandry) to Dilution water (test) has been shown not to
cause any detrimental effects for test daphnia, therefore no acclimation period of 48 h was
performed. This is a deviation from OECD Guideline No. 202 and EU-Method C.2, which
can be considered uncritical.
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- After 24 and 48 hours, the immobilised daphnia were counted.
Test conditions
- Hardness:
- 250 mg CaCO3/L
- Test temperature:
- 19.7 – 23.0°C
- pH:
- 7,6 (0h)
7.7 (48h) - Dissolved oxygen:
- 8.9 mg/L (0h)
8.6 mg/L (48h) - Nominal and measured concentrations:
- 1 µg/L (nominal concentration)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beakers, nominal volume 50 mL, tall shape
- Volume of solution: 50 ml
- Aeration: after preparation
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates):4
- No. of vessels per vehicle control (replicates):4
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: prepared water
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity: pH= 7.7
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium: M4-Medium (recipe of ELENDT)
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH: 7.7
- Photoperiod:
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
For the test concentration and the blank and solvent control, 20 daphnia were exposed to
the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised
daphnia were counted.
None of the animals was immobilised in the treatment or the blank / solvent control. There
fore no statistical evaluation was performed.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study to assure that the test conditions are reliable.
VEHICLE CONTROL PERFORMED: No
RANGE-FINDING STUDY
- Test concentrations: 1 µg/L
- Results used to determine the conditions for the definitive study: 1 µg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study (201902R201). The 24h-EC50 value was determined as 1.98 mg/L. The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of po
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: For the test concentration and the blank and solvent control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. None of the animals was immobilised in the treatment
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: For the test concentration and the blank and solvent control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. None of the animals was immobilised in the treatment
- Key result
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: For the test concentration and the blank and solvent control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. None of the animals was immobilised in the treatment
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: For the test concentration and the blank and solvent control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. None of the animals was immobilised in the treatment
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.79 µg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: For the test concentration and the blank and solvent control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. None of the animals was immobilised in the treatment
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.79 µg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: For the test concentration and the blank and solvent control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. None of the animals was immobilised in the treatment
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 0.79 µg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: For the test concentration and the blank and solvent control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. None of the animals was immobilised in the treatment
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.79 µg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: For the test concentration and the blank and solvent control, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. None of the animals was immobilised in the treatment
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control:no
- Immobilisation of control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels:
- Limit test:
- Dose-response test:
- ECx:
- Other: Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study to assure that the test conditions are reliable. - Reported statistics and error estimates:
- None of the animals was immobilised in the treatment or the blank / solvent control. There
fore no statistical evaluation was performed.
Any other information on results incl. tables
Table 9.1-a Biological Results Test Item
Parameter | Based on nominal values | Based on measured values |
24h EC50 | > 1 µg/L | > 0.79 µg/L |
48h EC50 | > 1 µg/L | > 0.79 µg/L |
48h NOEC | ≥ 1 µg/L | ≥ 0.79 µg/L |
48h LOEC | > 1 µg/L | > 0.79 µg |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- One valid experiment was performed.
The solubility of the test item in test medium is < 1 µg/L (see validation report
AB18062904G926). Therefore, this study was performed as a limit test at 1 µg/L to show
that there is no toxicity at the proposed LOQ (limit of quantification) of the analytical method. Acetone was used to spike the test solution. A stock solution containing 10 mg/L in
acetone were prepared. The concentration of the stock solution is 1000-fold higher than
the nominal concentration in the treatment (1 µg/L). For the solvent control, acetone in a
concentration of 100 µL/L was used.
For the test concentration and the blank and solvent control, 20 daphnia were exposed to
the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised
daphnia were counted.
None of the animals was immobilised in the treatment or the blank / solvent control. Therefore no statistical evaluation was performed.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions
was determined using LC/MS-MS-determination. All measured values are below the LOQ
(1 µg/L). At the beginning of the test, however, the measured raw data (area) clearly indicate that the test item is present in the solution. At the end of the test no test item was detectable. Due to the very low concentration, it is not clear what caused the loss of substance (hydrolysis, precipitation, or adsorption to the test organisms).
Therefore, the measured initial value is used for evaluation, even if it is below the LOQ.
In addition, the results are displayed in relation to the nominal values. This is in agreement
with the OECD guidance document no. 23 chapter 9: “For tests with chemicals that cannot
be quantified by the most sensitive analytical methods at relevant concentrations, the effect concentration can be expressed based on the nominal concentrations”.
No observations were made which might cause doubts concerning the validity of the study
outcome. All validity criteria were met.
The result of the test is considered valid
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.