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EC number: 227-565-8 | CAS number: 5891-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 9th November 2016 to 7th December 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Guideline study to GLP standards with no deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-chloropentan-2-one
- EC Number:
- 227-565-8
- EC Name:
- 5-chloropentan-2-one
- Cas Number:
- 5891-21-4
- Molecular formula:
- C5H9ClO
- IUPAC Name:
- 5-chloropentan-2-one
- Test material form:
- liquid
- Remarks:
- dark brown
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material:
5-Chloropentan-2-one (CAS-No.: 5891-21-4) Batch number 6U01818
- Purity test date:
06/09/2016
- Purity: 95.9%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
At 20 ± 5 °C, in the dark
Study design
- Oxygen conditions:
- aerobic/anaerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Rossdorf, Germany
- Method of cultivation: Aerobic activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage treatment plant
- Pretreatment: coarse particles were removed by settling for a short period (30 minutes) and then the upper layer decanted. The resulting activated sludge was aerated overnight until use.
- Concentration of sludge: 3.5 g/l - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 103.1 mg/L
- Based on:
- ThOD
- Remarks:
- NH4
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: standard mineral medium as per guideline
- Test temperature: 22°C ± 1°C
- pH: 7.6 (measured at the start of the test); 7.1 to 7.7 (measured at the end of the test)
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: test flasks containing a volume of approximately 500 ml
- Number of culture flasks/concentration: 2
- Measuring equipment: BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany
- Test performed in open system: No, according to test guidelines
- Details of trap for CO2 and volatile organics if used: Potassium hydroxide solution (45%) was used for trapping the produced carbon dioxide.
SAMPLING
- Sampling frequency: Daily
- Sampling method: The change of pressure in the test flasks was measured by means of a manometric method (BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany) each day.
CONTROL AND BLANK SYSTEM
- Inoculum blank: One flask
- Abiotic sterile control: One flask
- Toxicity control: One flask
- Other: Procedure control: One flask
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- None
- Test performance:
- Test criteria were all validated
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 76
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 66
- Sampling time:
- 13 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 23
- Sampling time:
- 3 d
- Details on results:
- 5-Chloropentan-2-one did reach a mean biodegradation of at least 10% at day 3, when the degradation was 23%. At the end of the 10-day window at day 13, the degradation was 66%. At the end of the test, the
degradation of 5-Chloropentan-2-one was 76% (ThODNH4).
In the toxicity control containing both, the test item and the reference item sodium benzoate, the biodegradation based on ThODNH4 was 61% after 14 days and 71% biodegradation after 28 days of incubation. According to the test guidelines the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
The oxygen demand in the abiotic control was 0 mg/L during the test duration.
BOD5 / COD results
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 89% after 14 days and to 97% after 28 days of incubation. The percentage biodegradation of the reference item confirms the suitability of the used aerobic activated sludge inoculum.
Any other information on results incl. tables
Cumulative Biochemical Oxygen Demand (mg O2/L) in Test Flasks during the Test Period
of 28 Days
Time (days) | Flask No. | ||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | |
1 | 0 | 0 | 0 | 0 | 55 | 0 | 55 |
2 | 0 | 5 | 5 | 0 | 80 | 0 | 75 |
3 | 45 | 45 | 5 | 5 | 95 | 0 | 95 |
4 | 70 | 65 | 10 | 5 | 120 | 0 | 130 |
5 | 80 | 75 | 10 | 5 | 130 | 0 | 135 |
6 | 95 | 85 | 10 | 5 | 135 | 0 | 145 |
7 | 105 | 95 | 10 | 5 | 145 | 0 | 155 |
8 | 115 | 105 | 10 | 5 | 150 | 0 | 165 |
9 | 120 | 110 | 10 | 5 | 150 | 0 | 175 |
10 | 125 | 110 | 10 | 5 | 155 | 0 | 185 |
11 | 125 | 115 | 10 | 10 | 155 | 0 | 195 |
12 | 130 | 120 | 10 | 10 | 160 | 0 | 210 |
13 | 130 | 125 | 10 | 10 | 160 | 0 | 220 |
14 | 135 | 130 | 10 | 10 | 160 | 0 | 225 |
15 | 135 | 130 | 10 | 10 | 165 | 0 | 235 |
16 | 135 | 135 | 10 | 10 | 165 | 0 | 245 |
17 | 140 | 135 | 10 | 10 | 165 | 0 | 250 |
18 | 140 | 140 | 10 | 10 | 165 | 0 | 255 |
19 | 140 | 140 | 10 | 10 | 165 | 0 | 255 |
20 | 145 | 140 | 10 | 10 | 165 | 0 | 255 |
21 | 145 | 145 | 10 | 10 | 170 | 0 | 260 |
22 | 145 | 145 | 10 | 10 | 170 | 0 | 260 |
23 | 145 | 145 | 10 | 10 | 170 | 0 | 260 |
24 | 145 | 145 | 10 | 10 | 170 | 0 | 260 |
25 | 145 | 145 | 10 | 10 | 175 | 0 | 260 |
26 | 145 | 145 | 10 | 10 | 175 | 0 | 260 |
27 | 145 | 145 | 10 | 10 | 175 | 0 | 260 |
28 | 145 | 145 | 10 | 10 | 175 | 0 | 260 |
Flasks 1 and 2: 5-Chloropentan-2-one
Flasks 3 and 4: inoculum control
Flask 5: reference (procedure control)
Flask 6: abiotic control
Flask 7: toxicity control
Percentage Biodegradation of the Test Item, of the Toxicity Control and of Sodium Benzoate
based on ThODNH4
Time (days) | Percentage Biodegradation 1 | |||
5-Chloropentan-2-one 1 | Sodium Benzoate 2 | Toxicity control 1,2 | ||
Flask 1 [%] | Flask 2 [%] | Flask 5 [%] | Flask 7 [%] | |
1 | 0 | 0 | 32 | 16 |
2 | 0 | 1 | 46 | 21 |
3 | 22 | 23 | 53 | 26 |
4 | 35 | 33 | 66 | 35 |
5 | 40 | 38 | 72 | 36 |
6 | 49 | 44 | 75 | 39 |
7 | 54 | 50 | 81 | 42 |
8 | 60 | 55 | 84 | 45 |
9 | 63 | 58 | 84 | 48 |
10 | 65 | 58 | 87 | 50 |
11 | 64 | 60 | 86 | 53 |
12 | 67 | 62 | 89 | 57 |
13 | 67 | 65 | 89 | 60 |
14 | 70 | 68 | 89 | 61 |
15 | 70 | 68 | 92 | 64 |
16 | 70 | 71 | 92 | 67 |
17 | 72 | 71 | 92 | 68 |
18 | 72 | 74 | 92 | 70 |
19 | 72 | 74 | 92 | 70 |
20 | 75 | 74 | 92 | 70 |
21 | 75 | 77 | 94 | 71 |
22 | 75 | 77 | 94 | 71 |
23 | 75 | 77 | 94 | 71 |
24 | 75 | 77 | 94 | 71 |
25 | 75 | 77 | 97 | 71 |
26 | 75 | 77 | 97 | 71 |
27 | 75 | 77 | 97 | 71 |
28 | 75 | 77 | 97 | 71 |
1 ThODNH4 of 5-Chloropentan-2-one: 1.725 mg O2/mg test item
2 ThODNH4 of sodium benzoate: 1.666 mg O2/mg reference item
pH-Values at the End of the Test
Flask No. | Treatment | pH-value |
1 | 5-Chloropentan-2-one | 7.1 |
2 | 5-Chloropentan-2-one | 7.1 |
3 | Inoculum control | 7.6 |
4 | Inoculum control | 7.6 |
5 | Reference item (procedure control) | 7.7 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The reliable OECD 301F Guideline study on ready biodegradability reported a degradation rate of 5-Chloropentan-2-one of 60% within the 10-day window and 76% after 28 days. Therefore, 5-Chloropentan-2-one is considered to be readily biodegradable.
- Executive summary:
The reliable OECD 301F Guidleine study on ready biodegradability was undertaken to GLP standards. Inoculum used was activated domestic sludge. Test conditions included test material, inoculum control, procedural control (sodium benzoate), abiotic control (CuSO4) and toxicity control (test material + sodium benzoate). Oxygen degradation was measured daily in sealed flasks using a BOD sensor.
The following results were determined:
5-Chloropentan-2-one reached a mean biodegradation of at least 10% at day 3, when the degradation was 23%. At the end of the 10-day window at day 13, the degradation was 66%. At the end of the test, the degradation of 5-Chloropentan-2-one was 76% (ThODNH4). Therefore, 5-Chloropentan-2-one is considered to be readily biodegradable based on ThODNH4.
Biodegradation of Sodium Benzoate: The reference item sodium benzoate was sufficiently degraded to 89% after 14 days and to 97% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
Biodegradation of the Toxicity Control: In the toxicity control containing both the test item and the reference item sodium benzoate, 61% biodegradation was noted within 14 days and 71% biodegradation after 28 days of incubation (based on ThODNH4). According to the test guidelines, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
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