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EC number: 225-625-8 | CAS number: 4979-32-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- (1998)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- - Dilution Water Source: Elendt M4 medium recommended by OECD TG 211 was used as dilution water.
- Dimethylformamide was used as solvent. 100uL/L of dimethylformamide was contained in the each test soltion.
- Stock Solutions Preparations and Stability: Test chemical was refrigerated. Each test solution was prepared as proper quantity of test chemical was dissolved in 1,000 mL of Elendt M4 contained dimethylformamide. Infrared absorption spectrum of the refrigerated test chemical was detected at the
start and the end of the test, and both spectrums are not contradictory to each other. Solvent was prepared as 40mg/10mL of dimethylformamide diluted to 10 times by dimethylformamide. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Age: < 24 hours old
- Supplier/Source: Test organisms were obtained from National Institute for Environmental Studies (Japan) and had been reproduced in the testing laboratory for 7 years.
- Any pretreatment: Parental daphnids were acclimated for 21 days on test conditions before testing, any groups showing high mortality were not used for testing. The mortality of the daphnids was less than 5% for 2 weeks before testing. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- 19.9 - 20.4ºC
- pH:
- 7.4 ¿ 8.5
- Dissolved oxygen:
- 7.4 - 8.8 mg/L
- Nominal and measured concentrations:
- - Nominal Concentrations: control, solvent control, 0.002, 0.0042, 0.009, 0.019 and 0.04 mg/L
- Time-weighted mean of measured concentration during 21 days: <0.00002, <0.00002, 0.00134, 0.00265, 0.00589, 0.0141, 0.0331 mg/l - Details on test conditions:
- - Exposure Vessel Type: 80 mL test solution in a 100mL glass beaker
- Number of Replicates: 10
- Individuals per Replicates: 10
- Renewal Rate of Test Water: everyday
- Light Condition: 16:8 hours, light-darkness
- Feeding: 0.15 mg carbon/day/individual (Chlorella vulgaris: Green Algae)
- Aeration: During test period, test solution did not aerated. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.033 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.033 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- At the highest concentration tested (0.0331 mg/l) no test organism was immobilized.
- Results with reference substance (positive control):
- EC50(48 hr, immobility) for a reference substance (potassium dichromate) was 0.89 mg/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- Successful positive control
- Conclusions:
- No effects up to the limit of the water solubilty.
- Executive summary:
The chronic toxicity of DCBS to aquatic invertebrates (Dapnia magna) was tested according to OECD TG 211 "Daphnia magna Reproduction test". After 21 days of exposure, an EC50 of >0.331 mg/L and a NOEC of 0.0331 mg/L were obtained (MITI, 2001).
Reference
The analysis of the test solutions showed that the ratio of the measured values to the nominal value, ±20%, was exceeded, so the time-weighted average of the measured values was used to calculate the results. The half-life in purified water was 30 hours (0.04 mg/L), and the main reason for the decrease was considered to be hydrolysis.
Result after 21 days exposure
(mg/L) | 95% confidence level (mg/L) | |
Half lethal concentration (LC50) of parent Daphnia magna | >0.0331 | Calculation not possible |
50% Reproductive Inhibitory Concentration (EC50 | >0.0331 | - |
No observed effective concentration (NOECi) | >0.0331 | - |
Minimum concentration of 100% inhibition | >0.0331 | - |
Description of key information
The chronic toxicity of DCBS to aquatic invertebrates (Dapnia magna) was tested
according to OECD TG 211 "Daphnia magna Reproduction test". After 21 days of exposure, an EC50 of >0.0331 mg/L and a NOEC of 0.0331 mg/L were obtained (MITI, 2001).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 0.033 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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