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EC number: 218-259-5 | CAS number: 2095-06-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Experimental test run under GLP and according to OECD and European Community guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- p-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline
- EC Number:
- 225-716-2
- EC Name:
- p-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline
- Cas Number:
- 5026-74-4
- Molecular formula:
- C15H19NO4
- IUPAC Name:
- 4-(oxiran-2-ylmethoxy)-N,N-bis(oxiran-2-ylmethyl)aniline
- Test material form:
- liquid: viscous
- Details on test material:
- - Substance type: organic
- Physical state: liquid
- Storage condition of test material: In refrigerator (2-8°C) in the dark
Constituent 1
- Radiolabelling:
- no
Study design
- Analytical monitoring:
- not specified
- Buffers:
- Acetate buffer pH 4, 0.1 M : solution of 16.6% 0.1 M sodium acetate and 83.4% 0.1 M acetic acid. The buffer contains 0.0009% (w/v) sodium azide.
Phosphate buffer pH 7, 0.1 M : solution of 0.1 M potassium dihydrogenphosphate adjusted to pH 7 using 10 N sodium hydroxide. The buffer contains
0.0009% (w/v) sodium azide.
Borate buffer pH 9, 0.1 M : solution of 0.1 M boric acid and 0.1 M potassium chloride adjusted to pH 9 using 10 N sodium hydroxide. The buffer contains 0.0009% (w/v) sodium azide.
- Number of replicates:
- 2
- Positive controls:
- no
- Negative controls:
- yes
Results and discussion
- Preliminary study:
- At pH 4, pH 7 and pH 9 a degree of hydrolysis of ≥ 10% after 5 days was observed :
- No test substance was detected in the blank buffer solutions pH 4 and pH 7.
- In the blank buffer solutions of pH9 a small response was observed at the test substance retention time, the contribution base on area to the samples at t=0 was 0.09%, this was negligible.
The mean recoveries of the buffer solutions fell within the criterion range of 70-110%. It demonstrated that the analytical method was adequate to support the hydrolysis study on the test substance. - Test performance:
- No test substance was detected in the blank buffer solutions at pH4, 7 and 9.
The mean recoveries of the buffer solutions relative to the stock solution fell within the criterion range of 90-110%. It demonstrated that the analytical method was adequate to support the hydrolysis study on the test substance. - Transformation products:
- not measured
Dissipation DT50 of parent compoundopen allclose all
- pH:
- 7
- Temp.:
- 20 °C
- Hydrolysis rate constant:
- 0.007 h-1
- DT50:
- 4.1 d
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 40 °C
- Hydrolysis rate constant:
- 0.068 h-1
- DT50:
- 10 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.163 h-1
- DT50:
- 4.3 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 4
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0.013 h-1
- DT50:
- 2.2 d
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: estimated based on Arrhenius equation
- pH:
- 7
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0.013 h-1
- DT50:
- 2.3 d
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: estimated based on Arrhenius equation
- pH:
- 9
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0.011 h-1
- DT50:
- 2.6 d
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: estimated based on Arrhenius equation
Any other information on results incl. tables
pH 4 | pH 7 | pH 9 | |||
Temperature [°C] | t½ | Temperature [°C] | t½ | Temperature [°C] | t½ |
20 | 3.9 d | 20 | 4.1 d | 20 | 4.6 d |
25 | 2.2 d | 25 | 2.3 d | 25 | 2.6 d |
40 | 10 h | 40 | 10 h | 40 | 12 h |
50 | 3.9 h | 50 | 4.3 h | 50 | 5.7 h |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The preliminary test (Tier 1) and main study (Tier 2) were performed for the determination of the rate of hydrolysis of p-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline at pH values normally found in the environment (pH 4-9). The test item was dissolved in aqueous solutions buffered at pH 4, 7 and 9. The solutions were incubated at 20 °C, 40 °C or 50 °C. The concentration of the test item was determined after different incubation times. A HPLC method was used for determination of the test item.
Summary of Results
rate half
temperature constant life
pH [°C] [h-1]
pH 4 20 0.007 3.9 d
pH 4 40 0.069 10 d
pH 4 50 0.176 3.9 h
pH 7 20 0.007 4.1 d
pH 7 40 0.068 10 h
pH 7 50 0.163 4.3 h
pH 9 20 0.006 4.6 d
pH 9 40 0.056 12 h
pH 9 50 0.122 5.7 h
- Executive summary:
Hydrolysis of p-(2,3-epoxypropoxy)-N,N-bis(2,3-epoxypropyl)aniline was studied at 20, 40 and 50 ºC in sterile aqueous buffered solutions at pH 4, pH 7 and pH 9. The hydrolysis at 25oC was estimated based on the Arrhenius equation.
The experiment was conducted in accordance with the EEC directive 440/2008, Guideline C.7 “Degradation - Abiotic Degradation: Hydrolysis as a Function of pH”,“ and the OECD Guideline 111 "Hydrolysis as a Function of pH”, adopted in April 2004 and in compliance with the GLP standards.Samples were analysed by HPLC.
pH 4 pH 7 pH 9 Temperature [°C] t½ Temperature [°C] t½ Temperature [°C] t½ 20 3.9 d 20 4.1 d 20 4.6 d 25 2.2 d 25 2.3 d 25 2.6 d 40 10 h 40 10 h 40 12 h 50 3.9 h 50 4.3 h 50 5.7 h This study is classified acceptable and satisfies the guideline requirement for hydrolysis study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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