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EC number: 215-523-1 | CAS number: 1328-51-4 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 74180.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03.04.2017 - 06.04.2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Experimental test result performed using standard test guideline
- Justification for type of information:
- Data is from ABITEC lab report.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Aim of this study was to assess the short term toxicity of Disulfo copper phthalocyanine amine salt (1328 -51 -4) to aquatic algae Desmodesmus subspicatus. Study was performed according to the OECD guideline in a static system for the total exposure period of 72 hrs.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: Disulfo copper phthalocyanine amine salt
- Molecular formula: C32 H14 Cu N8 O6 S2.2Na
- Molecular weight: 780.1706 g/mol
- Smiles notation: c1ccc\2c(c1)/c/3n/c-4n/c(n\c5c6c(cccc6S(=O)(=O)[O-])c/7n5[Cu]n3/c2n\c-8n/c(n7)/c9c8c(ccc9)S(=O)(=O)[O-])/c1c4cccc1.[Na+].[Na+]
- InChl: 1S/C32H16N8O6S2.Cu.2Na/c41-47(42,43)21-13-5-11-19-23(21)31-37-27-17-9-3-1-7-15(17)25(34-27)33-26-16-8-2-4-10-18(16)28(35-26)38-32-24-20(30(40-32)36-29(19)39-31)12-6-14-22(24)48(44,45)46;;;/h1-14H,(H2-2,33,34,35,36,37,38,39,40,41,42,43,44,45,46);;;/q-2;+2;2*+1/p-2
- Substance type: Organic
- Physical state: solid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- The stock solution 100 mg/L was prepared by dissolving dark blue powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml
- Method of cultivation: No data available
ACCLIMATION - No data available
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- ±1 hour
- Test temperature:
- 23±2°C
- pH:
- Test at highest concentration 100 mg/l: 7.9 (Change to 7.6 during tests)
Control: 8.3 (changed to 7.4 during test) - Nominal and measured concentrations:
- 0, 2, 4.4, 9.7, 21, 45, 100 mg/l nominal concentrations
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 37.1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI was 36 - 38.2 mg/l
- Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.75 mg/L - Reported statistics and error estimates:
- ErC50 was calculated using non-linear regression by the software Prism 4.0
- Validity criteria fulfilled:
- yes
- Conclusions:
- The median effective concentration (ErC50) for the test substance Disulfo copper phthalocyanine amine salt (1328 -51 -4), in Desmodesmus subspicatus was determined to be 37.1 mg/L on the basis of effects on growth rate in a 72 hour study.
- Executive summary:
Aim of this study was to assess the short term toxicity of Disulfo copper phthalocyanine amine salt (1328 -51 -4) to aquatic algae Desmodesmus subspicatus. Study was performed according to the OECD guideline in a static system for the total exposure period of 72 hrs.
Test conducted under the static system. The stock solution 100 mg/L was prepared by dissolving dark blue powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. 0, 2, 4.4, 9.7, 21, 45, 100 mg/l nominal concentrations were used.
Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration ErC50 was calculated using nonlinear regression by the software Prism 4.0.
The median effective concentration (ErC50) for the test substance Disulfo copper phthalocyanine amine salt (1328 -51 -4), in Desmodesmus subspicatus was determined to be 37.1 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the this value, indicates that the substance Disulfo copper phthalocyanine amine salt (1328 -51 -4) is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.
Reference
Description of key information
The median effective concentration (ErC50) for the test substance Disulfo copper phthalocyanine amine salt (1328 -51 -4), in Desmodesmus subspicatus was determined to be 37.1 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the this value, indicates that the substance Disulfo copper phthalocyanine amine salt (1328 -51 -4) is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 37.1 mg/L
Additional information
For determination of effect of chemical on algae study was carried out (from ABITEC lab report, 2017). Aim of this study was to assess the short term toxicity of Disulfo copper phthalocyanine amine salt (1328 -51 -4) to aquatic algae Desmodesmus subspicatus. Study was performed according to the OECD guideline in a static system for the total exposure period of 72 hrs.
Test conducted under the static system. The stock solution 100 mg/L was prepared by dissolving dark blue powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 min. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. 0, 2, 4.4, 9.7, 21, 45, 100 mg/l nominal concentrations were used.
Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration ErC50 was calculated using nonlinear regression by the software Prism 4.0.
The median effective concentration (ErC50) for the test substance Disulfo copper phthalocyanine amine salt (1328 -51 -4), in Desmodesmus subspicatus was determined to be 37.1 mg/L on the basis of effects on growth rate in a 72 hour study. Based on the this value, indicates that the substance Disulfo copper phthalocyanine amine salt (1328 -51 -4) is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 as per the CLP criteria.
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