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EC number: 212-480-0 | CAS number: 821-55-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Toxicity to micro-organisms study was conducted on Tetrahymena pyriformis for 48 hrs
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 2-Nonanone
- Molecular formula (if other than submission substance): C9H18O
- Molecular weight (if other than submission substance): 142.2402 g/mol
- Smiles notation (if other than submission substance): C(CCCCC)CC(C)=O
- InChI: 1S/C9H18O/c1-3-4-5-6-7-8-9(2)10/h3-8H2,1-2H3
- Substance type: Organic
- Physical state: Liquid - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 6 or more different test substance conc. was used for the study, exact conc. of test chemical was not reported.
- Sampling method: Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO) at conc. of 2.5, 5, 10, 25 or 50 mg/l.
- Sample storage conditions before analysis: No data available - Vehicle:
- yes
- Details on test solutions:
- Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO)
- Test organisms (species):
- Tetrahymena pyriformis
- Details on inoculum:
- No data available
- Test type:
- not specified
- Water media type:
- not specified
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No data available
- Hardness:
- No data available
- Test temperature:
- No data available
- pH:
- No data available
- Dissolved oxygen:
- No data available
- Salinity:
- No data available
- Nominal and measured concentrations:
- No data available
- Details on test conditions:
- TEST SYSTEM
- Test vessel: No data available
- Type (delete if not applicable): No data available
- Material, size, headspace, fill volume: No data available
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: No data available
- No. of vessels per concentration (replicates): Duplicates
- No. of vessels per control (replicates): Duplicates
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available
OTHER TEST CONDITIONS
- Adjustment of pH: No data available
- Photoperiod: No data available
- Light intensity: No data available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Population density was measured spectrophotometrically at 540 nm.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: Test chemical was tested in a range-finder prior to testing in duplicate for three additional replicates.
- Test concentrations: 6 or more different test substance conc. was used for the study, exact conc. of test chemical was not reported.
- Results used to determine the conditions for the definitive study: No data available - Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- other: IGC50
- Effect conc.:
- 449 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- No data available
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- The 50 percent growth inhibitory concentration, IGC50, was determined using Probit Analysis of Statistical Analysis System (SAS) software with Y as the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Toxicity to micro-organisms study was conducted onTetrahymena pyriformis for 48 hrs. Based on growth inhibition of test organism, the IGC50 value was found to be 449 mg/l.
- Executive summary:
Toxicity to micro-organisms study was conducted onTetrahymena pyriformis for 48 hrs.
Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO) at conc. of 2.5, 5, 10, 25 or 50 mg/l. The volume of stock solution added to each test flask did not exceed 0.35 ml, an amount that does not alterTetrahymena population growth.
Test chemical was tested in a range-finder prior to testing in duplicate for three additional replicates. Each replicate was set up using freshly prepared stock solutions.
After 48 hrs of exposure duration of test organism to test chemical 2-nonanone,population density was measured spectrophotometrically at 540 nm.
The 50 percent growth inhibitory concentration, IGC50, was determined using Probit Analysis of Statistical Analysis System (SAS) software withYas the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million.
Based on growth inhibition of test organism, the IGC50 value was found to be 449 mg/l.
Reference
Table: Log IGC50-1and IGC50 (mM) value of test substance to Tetrahymena pyriformis strain was given.
Chemical |
Log IGC50 -1 |
IGC50 (mM) |
2-nonanone |
0.50 |
3.163 |
Description of key information
Toxicity to micro-organisms study was conducted onTetrahymena pyriformis for 48 hrs.
Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO) at conc. of 2.5, 5, 10, 25 or 50 mg/l. The volume of stock solution added to each test flask did not exceed 0.35 ml, an amount that does not alterTetrahymenapopulation growth.
Test chemical was tested in a range-finder prior to testing in duplicate for three additional replicates. Each replicate was set up using freshly prepared stock solutions.
After 48 hrs of exposure duration of test organism to test chemical 2-nonanone,population density was measured spectrophotometrically at 540 nm.
The 50 percent growth inhibitory concentration, IGC50, was determined using Probit Analysis of Statistical Analysis System (SAS) software withYas the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million.
Based on growth inhibition of test organism, the IGC50 value was found to be 449 mg/l.
Key value for chemical safety assessment
Additional information
Four different experimental studies from peer reviewed journal for toxicity to micro organism endpoint for target chemical 2-Nonanone (Cas no. 821-55-6) are summarized as followed:
First experimental study suggest toxicity to micro-organisms study was conducted on Tetrahymena pyriformis for 48 hrs.Stock solutions of test chemical was prepared in dimethyl sulfoxide (DMSO) at conc. of 2.5, 5, 10, 25 or 50 mg/l. The volume of stock solution added to each test flask did not exceed 0.35 ml, an amount that does not alter Tetrahymena population growth.Test chemical was tested in a range-finder prior to testing in duplicate for three additional replicates. Each replicate was set up using freshly prepared stock solutions.
After 48 hrs of exposure duration of test organism to test chemical 2-nonanone,population density was measured spectrophotometrically at 540 nm.The 50 percent growth inhibitory concentration, IGC50, was determined using Probit Analysis of Statistical Analysis System (SAS) software with as the absorbency normalized as percentage of control and X as the toxicant concentration in parts per million. Based on growth inhibition of test organism, the IGC50 value was found to be 449 mg/l.
Another experimental study indicates the effects of 2 nonanone on Tetrahymena pyriformis was conducted under static condition. The test was allowed to run through 8-9 cell cycles. Semi defined proteose-peptone-based medium were inoculated to a density of 2500 cells/ml with log-growth-phase ciliates in the presence of the test material in DMSO. The test material was evaluated in each of three replicates at 6-8 different concentrations. Controls were inoculated with T. pyriformis in the absence of test material. Following incubation at 27 C, the population density of T. pyriformis was measured at 540 nM. The 50% growth inhibitory concentration was determined from probit analysis of absorbance values andconcentrations.IGC50 was found to be 33.26 mg/L under static condition for 2-Nonanone for 50% growth inhibition of Tetrahymena pyriformis.
Another experiment suggest In Toxicity to microorganisms study for 2-Nonanone was observed for Penicillium digitatum FRR 1562 and Colletotrichum musae DAR 24962.The MIC of each of the volatiles was evaluated and compound was classified as germicidal or germistatic in its effect on decay microorganisms. Agermicidal effect is the death of a microorganism, whereas a germ static effect is the inhibition of microbial replication). The agar disks of decay microorganisms which failed to grow due to the MIC of the compound were transferred onto new agar media free from the tested volatile and incubated for a further 5 days at 25 °C.The MIC observed with respect to 2 nonanone in Penicillium digitatum FRR 1562 was found to be 4.82 mmol/dish and for Colletotrichum musae DAR 24962 was found to be 3.58 mmol/dish.
And last experimental result suggest The 10 days toxicity study was conducted to evaluated to inhibitory effects of 2 nonanone.Erwinia carotovora UNSW 031700 was used as a test organism exposed to the concentration of 1.5ml/dish The NOEC was found to be 1.5m/dish for Erwinia carotovora.
Above all experimental values for Inhibition growth concentration (IGC50) and minimum inhibition concentration (MIC) conclude the target chemical 2-Nonanone (Cas no. 821-55-6) has no concern for toxicity to micro organism for acute exposure.
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