Benzyldimethyl(octadecyl)ammonium chloride

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From June 22, 1987 to July 28, 1987.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

Refer to the section 13 of IUCLID dataset for details on the read across justification. The algae study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

test media renewal after 48h. Analysis of the test substance was not performed.

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

test media renewal after 48h. Analysis of the test substance was not performed.

Principles of method if other than guideline:

Explanations and other deviations:
No chemical analyses but data from other tests demonstrate the stability and availability of the test substance under test conditions.
The second acclimatisation time is shorter than advised by the guideline but considering the low mortality during the second period. This is considered to have no impact on the final outcome of the test.

GLP compliance:

yes

Analytical monitoring:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Species/strain: Salmo gairdneri
Source: Forellenhof Fredesloh, Dr. O. Mueller, 3413 Moringen 1
Wild caught: no
Age/size: 5 ± 1 cm
Food: trout food Pelletgr. S1 (Rheinkrone Kraftfutter)
Pretreatment: during the first time of the adaptation phase (12d) the mortality came to 7% of the fish population. Therefore, the adaptation phase was extended for 5d. During this time the mortality only came to 0.5% of the fish population. 24h before the test started feeding of the fish was stopped.
Feeding of animals during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

ca. 14°C

pH:

ca. 8

Dissolved oxygen:

ca. 10 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0, 0.5, 0.7, 1.0, 1.4, 2.0, 3.5 and 10.0 mg/L.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.7 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.85 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1.75 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

During the first time of the adaptation phase (12d) the mortality came to 7% of the fish population. Therefore the adaptation phase was extended for 5d. During this time the mortality only came to 0.5% of the fish population. The oxygen content of the water was above 60% during the test and according to analyses in other tests (e.g. algae test), the test substance concentration was above 80% of the initial concentration during the test. Therefore all validity criteria are fulfilled. The concentration-response relationship appears to be typical for this type of substance. The study can be considered as valid.

Validity criteria fulfilled:

yes

Conclusions:

Based on the results of the read across study, the 96h LC0, LC50 and LC100 values (Oncorhynchus mykiss) for the test substance can be considered to be 1.4 mg/L (i.e., equivalent to 0.7 mg a.i./L), 1.7 mg/L (i.e., equivalent to 0.85 mg a.i./L) and 3.5 mg/L (i.e., equivalent to 1.75 mg a.i./L) (nominal).

Executive summary:

A study was conducted to determine the short-term toxicity to fish of the read across substance (active: 50%), C12-16 ADBAC, according to OECD Guideline 203 and EU Method C.1, in compliance with GLP. The experiment was conducted in Rainbow trout (Onchorhynchus mykiss) under static conditions. Fish (5 animals in duplicate) were exposed for 96 h to concentrations of 0, 0.5, 0.7, 1.0, 1.4, 2.0, 3.5 and 10.0 mg/L. No chemical analysis of test concentrations was performed. Abnormal findings during the exposure period were recorded in the swimming behaviour, visible physiological functions and outward appearance of the fish. Under the study conditions, the 96 h LC0, LC50 and LC100 values were determined to be 1.4 mg/L (i.e., equivalent to 0.7 mg a.i./L), 1.7 mg/L (i.e., equivalent to 0.85 mg a.i./L) and 3.5 mg/L (i.e., equivalent to 1.75 mg a.i./L) nominal (Dommrose, 1987). Based on the results of the read across study, similar the 96 h LC50 value can be considered for the test substance, C18 ADBAC, for short-term toxicity tofish.

Description of key information

Based on the results of the read across study, the 96h LC0, LC50 and LC100 values of the test substance, C18 ADBAC, for toxicity to fish is considered to be 1.4 mg/L (i.e., equivalent to 0.7 mg a.i./L), 1.7 mg/L (i.e., equivalent to 0.85 mg a.i./L) and 3.5 mg/L (i.e., equivalent to 1.75 mg a.i./L) respectively (nominal).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.85 mg/L

Additional information

A study was conducted to determine the short-term toxicity to fish of the read across substance (active: 50%) C12 -16 ADBAC, according to OECD Guideline 203 and EU Method C.1, in compliance with GLP. The experiment was conducted in Rainbow trout (Onchorhynchus mykiss) under static conditions. Fish (5 animals in duplicate) were exposed for 96 h to concentrations of 0, 0.5, 0.7, 1.0, 1.4, 2.0, 3.5 and 10.0 mg/L. No chemical analysis of test concentrations was performed. Abnormal findings during the exposure period were recorded in the swimming behaviour, visible physiological functions and outward appearance of the fish. Under the study conditions, the 96h LC0, LC50 and LC100 values of the read across substance in Oncorhynchus mykiss were determined to be 1.4 mg/L (i.e., equivalent to 0.7 mg a.i./L), 1.7 mg/L (i.e., equivalent to 0.85 mg a.i./L) and 3.5 mg/L (i.e., equivalent to 1.75 mg a.i./L) respectively (nominal) (Dommrose, 1987). Based on the results of the read across study, similar the 96 h LC50 value can be considered for the test substance, C18 ADBAC, for short-term toxicity to fish.